Applied Microbiology Research Papers/Topics

Detection of Extended Spectrum Beta-Lactamase Production in Escherichia coli Isolated from Cattle Faeces in Owo Metropolis

Escherichia coli which synthesize extended spectrum beta-lactamases (ESBL) have been implicated in severe human diseases. There is substantial evidence that cattle faeces have a role in developing and spread of multidrug-resistant pathogens, raising public health concerns. The study is aimed at detecting ESBL-producing E. coli associated with cattle faeces within Owo metropolis. Freshly passed faecal samples were taken aseptically from 9 apparently healthy cattle that were about to be sl...

Isolation and Identification of Bacteria in Broiler Chicken Meat Around Dar es Salaam Region

ABSTRACT Broiler chicken meat is now considered as a major fast-growing source of meat in many parts of Tanzania especially Dar es salaam. The consumers demand safe and hygienic products without contamination with pathogenic microorganisms when the production and consumption of broiler chicken meat is gradually increasing. The present study was conducted to assess the bacterial contamination of broiler chicken meat collected from different areas around Dar es salaam especially Temeke and...

Trends and Policy Correlates of Agricultural Production and Agricultural Production Sustainability Outcomes in Ghana and Nigeria

ABSTRACT This paper analysed policy correlates of agricultural production and agricultural production sustainability outcomes in Ghana and Nigeria. It underscores the influence of political systems and international development agendas as correlates of agricultural production and agricultural production sustainability outcomes. This is to the extent of providing evidence policy on agricultural production and agricultural production sustainability outcomes. Ghana and Nigeria have comparable fa...

Rationale For Vitamin C Treatment For Covid 19 and Other Viruses

Vitamin C and Coronavirus: Not a Vaccine; Just a Humble Cure Commentary by William F. Simmons and Robert G. Smith, PhD During the pneumonia epidemic in North Carolina in the 1940's, after American soldiers returned from the war in Europe, a small-town physician used an anti-infective protocol of intravenous vitamin C to cure 42 cases of viral pneumonia. [1] During the same period, the physician used a similar treatment to cure 60 cases of viral poliomyelitis. All patients were clinicall...

Rationale For Vitamin C Treatment For COVID-19 and Other Viruses

Vitamin C and Coronavirus: Not a Vaccine; Just a Humble Cure Commentary by William F. Simmons and Robert G. Smith, PhD During the pneumonia epidemic in North Carolina in the 1940's, after American soldiers returned from the war in Europe, a small-town physician used an anti-infective protocol of intravenous vitamin C to cure 42 cases of viral pneumonia. [1] During the same period, the physician used a similar treatment to cure 60 cases of viral poliomyelitis. All patients were clinically well...

Purification and Characterization of Lipase from Aspergillus flavus PW2961 using Magnetic Nanoparticles

________________________________(Received: 08:03:2017; Accepted: 05:04:2017) _______________________ Abstract Lipase from Aspergillus flavus was purified in a single step purification using MnFeO4 magnetic nano particles to achieve a 20.53- fold purification with specific activity of 11.29 U/mg and a 59% recovery yield. SDS-PAGE of lipase showed a single pure band with corresponding molecular weight of 35 kDa. The optimal temperature and pH for the enzyme activity were 45°C and 7.0 respe...

Evaluation of Inhibitory Zone Diameter (IZD), Phytochemical Screening, Elemental Composition and Proximate Analysis of Crude Cleistopholis Patens Leaf

This purpose of this study is to determine the Inhibitory zone diameter (Izd), phytochemical screening, elemental composition and proximate analysis of crude Cleistopholis patens leaf, bark, aqueous and ethanol extracts on selected pathogenic isolates. The leaf and bark of Cleistopholis patens plants were obtained from a location in the southwestern part of Nigeria, in the tropical rainforest of Ikare Akoko, Ondo state and Ile Ife, Osun state, Nigeria. The six (6) bacterial isolates include E...

IN VITRO EVALUATION OF THE ANTIMICROBIAL POTENCY

 The search for potent antimicrobial agent is unending. The use of mouthwashes to improve oral hygiene is being favoured for its presumed antimicrobial activity. This study therefore evaluated the antibacterial activity of common mouthwashes against relevant clinical isolates.  Five mouthwash samples (coded; LIS; COL; TOT; FLU; BRE) bought from major super markets in Ondo State, Nigeria, were examined for antimicrobial activity using agar well diffusion method. The kinetic of activity and M...

Evaluation of the viability and survival rates of some selected pathogens

Artificially inoculated fruit juice is a very viable profit making sole business in Nigeria, the product is locally available in all market in different parts of the country, especially in Ondo state, using Ikare and Iwaro Akoko market, as the focal point of this present study. Fifty (50) samples of the inoculated fruit juice were purchase from the market in Iwaro and Ikare Akoko, Ondo State. The test organisms used for this research work are Staphylococcus aureus (ATCC) 25923, Salmonella spp...

Comparative Studies of the Efficiency of Sun Drying and Hot Air Oven

The drying efficacy and product quality of onion and pepper dried in the oven and sun were compared. The drying process took 1-4 days to accomplish. The proximate analysis, bacterial load and identification of microorganisms, sugar fermentation, catalyses test and the bacteria count were determined. The mean moisture content of the product at the end of the drying was 6.4% (sun) and 4.5% (oven). The oven dried product were significantly (P

Bioactivity and Phytochemical Screening of Nigerian Medicinal

The Aqueous, ethanol, hexane and ethyl acetate extracts of the leaves, bark and roots of Acacia albida, Anchomanes difformis, Boscia senegalensis, Bridelia ferruginea, Ficus ingens, Indigofera arrecta and Moringa oleifera were tested for possible source of antibacterial activities against selected clinical organisms found in the pediatrics hospital in Ondo and Ekiti State, Nigeria. The selected clinical organisms used for this research work are Salmonella typhi, Staphylococcus aureus, Klebsie...

Use of the Biomerieux id 32c Fungal Identification System for Fungal Isolated from Mother and Child Hospital Dump Site in Akure, Ondo State Nigeria

The purpose of this research work is to isolate and identify Using ID 32C API kit and to determine the antifungal sensitivity profile of fungi isolated from the mother and child dump site Akure, Ondo state, Nigeria.   Ten isolates (Yeast, Aspergiliusflavus, Aspergiliusniger, Fusariumsolani, Candida albucan, Candida kruise, Phytoptera, Rhizopusstolonifer, Trichodermhorizianum,Fusariumvortezelium) were used for this research work.Potato Dextrose Agar was used to isolate and prepared for enume...

Bio-prospective Screening of Antibacterial and Phytochemical Activity of Caesalpinia pulcherrima.Linn (Pride of Barbados) on Selected Clinical Isolates

This work was carried out to evaluate the antibacterial and Phytochemical activities of Caesalpiniapulcherrima  leaf and stem bark on some clinical isolates. The plant parts were collected from St. Benedict Catholic Church premises, Owena Barracks, Akure, Ondo State. Crude ethyl acetate extracts of Caesalpinia pulcherrima leaves and stem bark was assayed for its antibacterial activity against Staphylococcus aureus, Klebsiella pneumonia, Pseudomonas aeruginosa, Bacillus subtilis, Escheric...

Prospects of Essential Oils in Drug Discovery

Abstract Essential oils (EO), according to the International Standard Organization (ISO)  defines essential oil as the product obtained from parts of plants by distillation with water steam and the product obtained by expression from pericarp of medicinal plant. The rapid growth and increase of the knowledge of identification, extraction, purification and characterization of essential oils in most local plant species is fast becoming an important aspect of the drug production process. ...

Evaluation of Antimicrobial Activity, Synergistic Efficacy, Qualitative and Quantitative Phytochemical Determination of Alstonia Boonei Leaf and Stem Bark on Selected Clinical Isolates

Abstract This study evaluated the antimicrobial activity, of Alstonia boonei leaf, stem bark and the synergistic efficacy of A. boonei leaf plus stem bark on seven bacteria (Gram positive and Gram negative bacteria) which are strains of Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Proteus viridans, Salmonella typhi and Klebsiella pneumoniae, and three fungal isolates which are Candida albicans, Aspergillus flavus and Trichophyton rubrum. This study also ...


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