Botany Research Papers/Topics

PROTOCOL OPTIMIZATION FOR IN VITRO PROPAGATION OF THREE SELECTED ENSET (Ensete ventricosum (Welw.) Cheesman) VARIETIES; YANBULE, MESENA AND ENDALE THROUGH SHOOT TIP CULTURE

Abstract: Enset (Ensete ventricosum (Welw.) Chessman) has socio-cultural, ethno-medicinal and economic use values. Nearly all enset genotypes are being threatened by bacterial wilt disease upon conventional propagation. Therefore, this research was designed to develop mass in vitro propagation protocol for three elite enset varieties from shoot tip explants. The experiment was laid out in CRD with three replications in factorial arrangement. Apart from ethanol (70%), sodium hypochlorite (NaO...

GREEN SYNTHESIS OF SILVER NANOPARTICLES USING VERBENA OFFICINALIS L. ROOT EXTRACTS AND THEIR ANTIBACTERIAL ACTIVITY AGAINST ESCHERCHIA COLI AND STAPHYLOCOCCUS AUREUS

Abstract: Green nanoparticle synthesis techniques for various biological activities and medical applications are desired because of their bio-compatibilit. Hence, in this study, silver nanoparticles (AgNPs) were synthesized using root extracts (aqueous, methanol and hexane) of V.officinalis. The antibacterial efficacy of the nanoparticles was assayed by the Kirby–Bauer disc diffusion method against bacterial pathogens such as Escherichia coli and Staphylococcus aureus. The minimum inhibito...

THE ROLE OF NEEM (Azadirachta indica) SEED AND GARLIC (Allium sativum) BULB POWDERS AS BIO-PESTICIDES AGAINST ADULT MAIZE WEEVIL (Sitophilus zeamais Motsch.) IN STORED MAIZE

Abstract: Post-harvest losses are one of the major causes of food insecurity in the developing world. The present study was planned to investigate the effect of neemseed and garlic bulb powders in control of maize weevil and prolonged storage of maize grains. A 3x3x2 factorial experiment was laid in a Complete Randomized Design (CRD) replicated two times. The result indicated that all the treatments had shown high repellence against maize weevil at all amounts of treatments (5, 10, and 15 gr...

ARBUSCULAR MYCORRHIZAL FUNGI ABUNDANCE AND DIVERSITY ACROSS DIFFERENT LAND-USE TYPES IN JABI TEHNAN WOREDA WESTERN GOJAM, ETHIOPIA

Abstract: Arbuscular mycorrhizal fungi (AMF) are ubiquitous soil organisms. They form symbioses with majority of terrestrial plants and contributor to mineral and water uptake, plant biodiversity, productivity, ecosystem stability and function. However, AMF community structure and function are affected by land use and land cover changes. This necessitates the exploration of the AMF land- use change association within selected agro-ecosystems in the country. To this end, a site was selected a...

MICROPROPAGATION OF ENDOD (Phytolacca dodecandra) L ′Herit THROUGH LEAF CULTURE

Abstract: Endod (Phytolacca dodecandra L’Heit var. E-44 and E-17) is medicinal plant of high saponin content used as molluscicide to control Schistosomiasis. The aim of, this study was to develop a micropropagation protocol for P.dodecandra. The E17 and E44 variety leaf explant was sterilized and cultured on MS media supplemented with different types of concentration and combination of cytokinins and auxins for shoot initiation, multiplication and root initiation. Local bleach (Berekina) 3...

PHYTOCHEMICAL SCREENING FROM LEAVES OF DATURA STRAMONIUM L. AND JUSTICIA SCHIMPERIANA AND EVALUATION OF THEIR ANTIMICROBIAL PROPERTIES AGAINST SELECTED HUMAN ENTERIC BACTERIA

Abstract: The growing phenomenon of antibiotic resistance, particularly to pathogenic microorganisms, in current medicine, has directed the concern of scientists for finding novel antimicrobial agents from plant origin with negligible side effect. The aim of this study was to screen the major secondary compounds of leaves of D. stramoinum and J. schimperiana and to evaluate their antimicrobial properties against (S. aureus, S. typhi and S. boydii). Extraction was done by maceration of leaf p...

PHYTOTOXICITY OF Lantana camara L. EXTRACTS AND SOIL FROM BENEATH ITS CANOPY ON CABBAGE (Brassica carinata A.)

Abstract: Lantana camara is an invasive weed species that has allelopathic effects on crops and other indigenous associated plants. Allelochemicals from such plants may also have positive or negative impact on soils. Therefore, the research was carried out to investigate the allelopathic effect of leaf extracts of Lantana camara and soils invaded by it on Brassica carinata. Leaf extracts of Lantana camara were prepared with water, hexane and methanol, while distilled water alone was used as ...

ETHNOBOTANICAL STUDY OF MEDICINAL PLANTS USED BY PEOPLE OF GUMER WOREDA, GURAGE ZONE,SNNPR, ETHIOPIA

Abstract: Ethnobotanical study of medicinal plants used by local people and associated indigenous knowledge was conducted in Gummer woreda, Gurage Zone, SNNPRS of Ethiopia. The purpose of this study was to investigate and document the traditional medicinal plants used by indigenous people of Gummer Woreda together with indigenous knowledge. Data collection tools were semi-structured interviews, field observations and group discussions. Descriptive statistics informant consensus factor, fidel...

ETHNOBOTANICAL STUDY OF MEDICINAL PLANTS USED BY THE PEOPLE OF TARMABER DISTRICT, NORTH SHEWA ZONE, AMHARA REGION, ETHOPIA

Abstract: Ethiopia has rich flora with different plant species having use in health care system based on local indigenous knowledge. In this study, plants of traditional medicinal use and their associated indigenous knowledge in Tarmaber district were investigated. A total of 100 informants (age≥20) were selected to collect information on medicinal plant use from four sampled kebeles. Of these, 30 key informants were selected purposively based on recommendation by local elders and authorit...

FLORISTIC COMPOSITION, STRUCTURAL ANALYSIS AND REGENERATION STATUS OF WOODY PLANT SPECIES IN DABAL FOREST, EAST HARARGHE ZONE, OROMIA, ETHIOPIA

Abstract: The study was conducted in Dabal forest to investigate the floristic composition, population structure and regeneration status of woody plant species (WS) in the forest. 52 nested quadrats of the size 20m × 20m, 10m x 10m and 5m × 5m for tree/shrub, sapling and seedling respectively, were laid systematically along seven line transects. For each quadrat, variables such as altitude, longitude and latitudes were recorded by using Garmin 62 GPS. A metermarked stick was used to measur...

EVALUATION OF ALLELOPATHIC EFFECT OF Lantana camara L. LEAF EXTRACTS AND GROWTH INHIBITORY EFFECT OF SOIL FROM BENEATH ITS CANOPY ON Lepidium sativum L.

Abstract: In most cases invasive plants have allelopathic effects on crops and other indigenous associated plants. Allelochemicals from such plants may also have negative impact on soils. This study was carried out to investigate the allelopathic effect of leaf extracts of Lantana camara and soils invaded by it on Lepidium sativum. Leaf extracts of Lantana camara were prepared in 5, 10, 15 and 20% concentration levels while distilled water was used as control to evaluate germination paramete...

ETHNOBOTANICAL STUDY OF TRADITIONAL MEDICINAL PLANTS IN ADAMI TULU JIDO KOMBOLCHA DISTRICT, OROMIA, ETHIOPIA

Abstract: Ethiopia has rich flora with different plant species having medical importance in health care system based on local indigenous knowledge. In this study, plants of traditional medicinal use and their associated indigenous knowledge in Adami Tulu Jido Kombolcha were investigated. A total of 100 informants (age≥25) were selected to collect information on medicinal plant use from three sampled kebeles. Of these, 20 key informants were selected purposively and 80 informants were selec...

ETHNOBOTANICAL STUDY OF MEDICINAL PLANTS USED BY PEOPLE OF GOG DISTRICT, AGNUAK ZONE, GAMBELLA REGIONAL STATE, ETHIOPIA

Abstract: Ethnobotanical study of medicinal plants was conducted to document the indigenous knowledge in Gog District, Agnuak Zone, Gambella Regional State, South Western Ethiopia. A total of 74 informants (age≥25) were selected to collect information on medicinal plant use from five sampled Kebeles. Of these, 14 key informants were selected purposively based on recommendation by local elders and authorities. The rest were selected randomly. Data were collected using semi-structured interv...

Medicinal Plants Used by the People of Hulet Ejju Enesie Woreda, East Gojjam Zone of Amhara Region, Ethiopia

Abstract: Medicinal plants have been used as a source of medicine to treat human and livestock ailments since time immemorial. Indigenous people of a given community have their own local specific knowledge on plant. This study was conducted to document indigenous plant based medicinal knowledge in the study area. The data were collected using semi structured interviews, focus group discussions and field observations with local peoples. A total of 100 informants (70 males and 30 females) abov...

EFFECTS OF NEEM LEAF EXTRACT DIPPING AND BEESWAX COATING ON SHELF LIFE AND QUALITY OF TOMATO (Lycopersicon esculentum Mill.) FRUITS

Abstract: Tomato fruit is highly perishable due to its climacteric ripening nature. The high postharvest loss of tomato in Ethiopia is also because of poor harvesting techniques, lack of packaging, inappropriate transportation, storage and absence of cooling facilities. Experiment was carried out to investigate the efficacy of beeswax (BW) emulsion and neem leaf extract (NLE) on elongation of shelf life and maintenance of quality of harvested tomato fruits. Freshly harvested mature green tom...


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