Biotechnology Research Papers/Topics

Isolation and Characterization of Rhizobia from Leguminous Plants and Assessing their Plant Growth Promoting Properties

Abstract: The increasing world population led to mass application of chemicals in agriculture to increase food production and meet the demand. Today, attentions are diverted into biofertilizers than the chemical fertilizers. This study was initiated to isolate and characterize rhizobia from Faba bean (Vicia faba L.) and assessment for plant growth prompting substance. Faba bean samples were collected by systematic random sampling from farmers’ fields of three geographically varied location...

Invitro Regeneration of Echinops Kebericho from Shoot Explants

Abstract: Echinops kebericho is an erect massive rootstock bearing perennial herbs or shrub belonging to the family Asteraceae. Echinops kebericho, endemic to Ethiopia is a critically endangered medicinal plant. It is among few important medicinal plants of the country valued primarily for root parts. The seeds of Echinops kebericho characterized by short viability period and lose their capacity to germinate very quickly. This study aimed to develop in vitro regeneration of Echinops keberich...

ISOLATION AND SCREENING OF SELECTED HEAVY METAL RESISTANT BACTERIA FROM KOMBOLCHA TANNERY INDUSTRY EFFLUENT

Abstract: This study was aimed to replace the chemical and physical treatment with biological treatment to save the environment. It was concerned with the isolation, screening, and charact erization of heavy metal-resistant bacteria isolated from Kombolcha tannery industry effluent. Different ways were used to select the maximum of isolates resistant to heavy metals. Based on, morphological, gram stain and biochemical tests six resistance bacteria were isolated by using free nutrients agar m...

THE IMPACT OF TEMPERATURE PRETREATMENT ON BIOGAS YIELD FROM SAWDUST, CO-DIGESTED WITH COW DUNG

Abstract: Renewable resources play a significant role in reducing carbon dioxide emissions around the world, and with this in mind, biomass and waste is a candidate to be one of the most widely utilized renewable energy resources. Therefore, this research was conducted to investigate the production of biogas from saw dust, cow dung in solo or mix through anaerobic digestion with five treatment ratios, under mesophilic condition (38ºC) using batch digesters at Haramaya University for 30 days...

BIOETHANOL PRODUCTION VIA FERMENTATION OF BANANA PEEL USING Saccharomyces cerevisiae

Abstract: Bioethanol derived from biomass contributed 10 to 14% of the total world energy supply and solved the world crisis such as global warming and depletion of fossil fuels. Therefore, this research is initiated to produce ethanol from biomass such as banana peel which was fermented bcy different concentration of substrates with 0.5% and 1% yeast in different fermentation period’s day, with the aim of optimizing the substrate concentration for ethanol production. The sample (Banana pe...

PRODUCTION OF BIOORGANIC LIQUID FERTILIZER FROM COW MANURE AND GROUNDNUT HUSK

Abstract: Organic Liquid Fertilizer made from the decomposition of organic material such as plant residue and animal waste through anaerobic fermentation comprises number of growth substances, vitamins, antibiotics, amino acids and useful micro-organisms. The advantage of these fertilizers resolve a deficient of nutrient and provide the additionally faster nutrient supply. Thus, the present study was aimed to produce organic liquid fertilizer from cow manure and groundnut husks through aerob...

PREPARATION OF BIOORGANIC LIQUID FERTILIZER FROM CHICKEN MANURE AND GROUNDNUT HUSK

Abstract: Natural organic fertilizers used in plant cultivation are characterized by low efficiency and soil-applied in large quantities to obtain high yield. The bioorganic fertilizer obtained through anaerobic fermentation comprises number of growth substances, vitamins, antibiotics, amino acids and useful micro-organisms. Bioorganic liquid fertilizer not only increases bioorganic fertility of crops (in comparison to the control and prototype fertilizer), but also accelerates their maturat...

THE EFFECT OF CO-DIGESTION ON BIOGAS PRODUCTION WITH Bidens pilosa L. AND POULTRY MANURE THROUGH ANAEROBIC DIGESTION

Abstract: Biogas is an eco-friendly renewable energy source through the anaerobic digestion of organic waste materials. In this study, biogas production from co-digestion of Bidens pilosa(BP) and poultry Manure (PM) in five mix ratios (100%BP, 75%BP:25%PM, 50%BP:50%PM, 25%BP:75%PM and 100%PM) were evaluated under at 38ºC using a batch mode digester over 21 days of fermentation. In all treatments, physico-chemical parameters such as pH, total solid, volatile solid, moisture content, organic ...

PRODUCTION OF ALKALINE PROTEASE FROM AVOCADO (Persea americana Mill.) SEED AND PEEL WASTES USING FUNGI

Abstract: Microbial proteases are hydrolytic enzymes that are widely used in many industrial processes. This study aimed to evaluate the production of alkaline protease (AP) from fungal isolates sourced from avocado (Persea americana Mill.) peel (AVP) and avocado seed (AS) under solid state fermentation (SSF). AVP and AS was collocated form Harar juice house into plastic bag and serial diltuion method was used for fungal isolatin. The isolates were subutured on potato dextrose agar (PDA) and...

Production of biogas from Co-Digestion of Goat Manure and Watermelon (Citrullus lanatus) Fruit Peels under Anaerobic condition

Abstract: Biogas production is one of the technologies that can overcome the problems related to energy Crisis. Therefore, this research was conducted to investigate the biogas production potential of Xanthium strumarium leaves in combination with goat manure in anaerobic condition. Completely randomized experimental design was used under mesophilic condition (38ºC). Five batch fermentation treatments (T1=100% goat manure, T2=75% goat manure:25% Xanthium strumarium, T3=50% goat manure:50% X...

PRODUCTION OF PECTINASE FROM BARLEY (Horedium vulgare Von.Bothmer) AND WHEAT (Triticum turgidum L.Var) HULLS

Abstract: Pectinase is a heterogeneous group of enzyme which hydrolyzes pectin. Fungi and actinomycetes have ability to produce a large amount of industrial enzymes. Therefore the present study was undertaken production of pectinolytic enzymes from barley (Hordeum vulgare Von. Bothmer) and wheat (Triticum turgidum L.Von) locally called (Garbu or Qamadi) hulls. Wheat and Barley Hulls samples were collected from homes. Isolation of the fungal species was done on czapek-dox-agar media. Fungal i...

BIOGAS YIELD FROM INVASIVE WEED (Ageratum conyzoides L.) CO-DIGESTED WITH COW MANURE UNDER ANAEROBIC CONDITION

Abstract: Energy is one of the most important factors for human development and global economic growth. Renewable energy techniques give a great opportunity for the reduction of greenhouse gas emissions and decreasing global warming via replacing traditional energy sources. Biogas is a clean, environmental friendly and renewable form of energy generated when micro organisms degrade organic materials in the absence of oxygen. This study was aimed to evaluate the biogas production potential of...

FUNGAL PRODUCTION OF ALKALINE PROTEASE FROM ABATTOIR AND FOOD WASTES

Abstract: Alkaline proteases are one of the classes of protease enzymes which are extensively used in detergent and tanning industries. Currently, there is an increasing demand for biocatalysts from extreme environments that are important sources of microorganisms routinely used in novel industrial enzyme production. In this research attempts were made to isolate and screen for an alternative alkaline protease producing fungal species from abattoir and food wastes. Hence, fungal species were...

Evolution and Diagnostic Potential of Major Surface Proteases from Trypanosoma Vivax, T.Brucei Brucei and T.Congolense

Abstract: Tsetse-transmitted trypanosomiasis is a disease unique to Africa, affecting both humans and animals. This disease occurs in about 10 million km2 in 37 sub-Saharan countries corresponding approximately to one-third of Africa's total land area, and threatens an estimated 50 million people and 48 million cattle. The estimated annual losses in cattle production alone are in the range of 1.0-1.2 billion dollars. The diagnosis of trypanosomiasis is notoriously difficult as there are no s...

Molecular Identification and Characterization of Spiroplasma in Anopheles Arabiensis from Kenya

Abstract: Vector-borne diseases (VBDs) are a devastating global health problem. Mosquitoes are amongst the most important vectors of human VBDs. Several measures have been put in place to manage and eliminate these vectors, however they have all faced a variety of setbacks and raising the need to develop other methods of control. The use of bacterial endosymbionts is a highly promising new method to explore for this purpose. This study aimed to identify and characterize Spiroplasma, a matern...


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