Biochemistry Study Materials

BCH 307 Regulation of Nucleic Acid and Lipids Metabolism

Nucleic Acid Metabolism Nucleotides  Nucleotide Structure Regulation of Purine Biosynthesis  Regulation of Pyrimidine Biosynthesis Regulation of Lipids Metabolism Digestion and Absorption of Lipids Triglyceride Storage & Mobilization Hormonal control of lipolysis Glycerol Metabolism Lipids Disorder Lipids Deficiency Lipids Exceeding Cholesterol Lipoproteins Blood levels for Lipids Prevention of Lipid Disorder

BCH 307 Intermediary Metabolism II (Lipid digestion absorption and transportation)

Lipids Dietary fat Composition Fat Metabolism Emulsification and digestion Digestion of lipids Bile salt TAG degradation by pancreatic lipase Cholesteryl ester degradation Phospholipid degradation Phospholipase A2 Fate of TAG

BCH 303 Enzyme kinetics

Introduction  Basis Michaelis-Menten equation Assumption of equilibrium Assumption of steady state Significance of Km Analysis of Kinetic Data Lineweaver-Burk Plot Lineweaver-Burk equation Interpreting the LB plot Eadie-Hofstee plot Eadie-Hofstee equation Hanes plot Hanes–Woolf equation

BCH 303 - Introduction to Enzymology

Enzyme Assay, Purification and Cell Disruption Hexokinase and glucose oxidase Uricase and cholesterol esterase NEED FOR ENZYME PURIFICATION STEPS IN ENZYME PURIFICATION Microbial source Heterologous enzymes in microbes Plant source Animal source Methods for initial recovery Removal of cell debris and whole cells Removal of nucleic acids and lipids Concentration of enzymes Final purification by column chromatography

Plant Diversity and Function (PLB 108)

Plant Diversity and Function (PLB 108) - Lecture Note The Evolution of Today's PlantsEvolution of the Conducting Cells of Vascular SystemsAngiosperms: Flowering PlantsGeneral Problem in Plant FunctionUptake and Movement of Water & Solutes in PlantsTransport of Water and Minerals in the Xylemetc

CPY 301: Metabolism of Carbohydrates

INTRODUCTION Metabolism is simply defined as the total sum of enzyme catalyzed chemical reactions that take place in living cells. All living cells irrespective of their origin (be it plants, animals and micro-organisms) participate in metabolism, which is responsible for the coordination of all activities carried out by these organisms.  Metabolism is in two phases: anabolism (synthetic) and catabolism (degradative) phases. Anabolism undergoes the following:Reductive reactions.Starts from k...