The stem borer (Chilo partellus) is one of the major constraints in maize and sorghum production worldwide. Control of Chilo partellus is mainly done through synthetic insecticides which are very expensive and have negative effects to environment and non-target organisms. Farmers in Mbeere use Carphalea glaucescens and Gnidia glauca in control of Chilo partellus because of the high cost of conventional insecticides. However, there is lack of scientific information on the their mode of action is not known. This study was designed to gather preliminary data that can be used to develop a bio insecticide to control the Chilo partellus. Aqueous and dichloromethane leaf extracts from Carphalea glaucescens and Gnidia glauca were assayed in vitro for their activities against Chilo partellus anti-acetylcholinesterase activity. Acetylcholinesterase is one of the most efficient enzymes of nervous system which is concentrated at the cholinergic synapses and at neuromuscular synapses where it rapidly hydrolyses the neurotransmitter acetylcholine. Acetylcholinesterase plays a critical role in terminating synaptic transmission so that the next nerve impulse can be transmitted across the synapse. Plants were collected from Siakago, Mbeere North sub-county in Embu County, Kenya. Chilo partellus were obtained from KALRO (Katumani) and the crude enzyme acetlycholinesterase extracted through homogenizination. Activity of the isolated crude enzyme was determined as described by Ellman et al. (1961). Acetylthiocholine iodide was used as a substrate in the assay.
Anne, N (2021). Anti-acetylcholinesterase Activities Of Leaf Extracts Of Carphalea Glaucescens And Gnidia Glauca From Mbeere North Subcounty, Kenya On Chilo Partellus Larvae. Afribary. Retrieved from https://afribary.com/works/anti-acetylcholinesterase-activities-of-leaf-extracts-of-carphalea-glaucescens-and-gnidia-glauca-from-mbeere-north-subcounty-kenya-on-chilo-partellus-larvae
Anne, Njoroge "Anti-acetylcholinesterase Activities Of Leaf Extracts Of Carphalea Glaucescens And Gnidia Glauca From Mbeere North Subcounty, Kenya On Chilo Partellus Larvae" Afribary. Afribary, 05 Jun. 2021, https://afribary.com/works/anti-acetylcholinesterase-activities-of-leaf-extracts-of-carphalea-glaucescens-and-gnidia-glauca-from-mbeere-north-subcounty-kenya-on-chilo-partellus-larvae. Accessed 25 Apr. 2024.
Anne, Njoroge . "Anti-acetylcholinesterase Activities Of Leaf Extracts Of Carphalea Glaucescens And Gnidia Glauca From Mbeere North Subcounty, Kenya On Chilo Partellus Larvae". Afribary, Afribary, 05 Jun. 2021. Web. 25 Apr. 2024. < https://afribary.com/works/anti-acetylcholinesterase-activities-of-leaf-extracts-of-carphalea-glaucescens-and-gnidia-glauca-from-mbeere-north-subcounty-kenya-on-chilo-partellus-larvae >.
Anne, Njoroge . "Anti-acetylcholinesterase Activities Of Leaf Extracts Of Carphalea Glaucescens And Gnidia Glauca From Mbeere North Subcounty, Kenya On Chilo Partellus Larvae" Afribary (2021). Accessed April 25, 2024. https://afribary.com/works/anti-acetylcholinesterase-activities-of-leaf-extracts-of-carphalea-glaucescens-and-gnidia-glauca-from-mbeere-north-subcounty-kenya-on-chilo-partellus-larvae