The impact of extraction methodology and polarity of extraction solvents on the assay of phenolic compounds was investigated using musk tree seed (Buchholzia coriacea) as a model substrate. The efficiency of four solvents (methanol, ethanol, acetone and ethyl acetate) at 20, 40, 60, 80 and 100% aqueous solution and five extraction methods (shaking, vortex mixing, stirring, sonication, sonication/vortexing and accelerated solvent extractor) in extracting the phenolics of B. coriacea were evaluated. Extracts were analyzed for phenolic content by high-performance liquid chromatography and Folin–Ciocalteu assays. Two major phenolic peaks (1 and 2) were isolated from the B. coriacea seed extract in the HPLC which were suspected to be lupeol and β- sitosterol. Methanol at 20% aqueous solution was found to be most efficient in extracting the total phenolic and HPLC peak 1 component of B. coriacea whereas acetone at 80% aqueous solution was more efficient in extracting peak 2 component. On the other hand Accelerated Solvent Extractor (ASE) extraction procedure was noted to be most efficient for extracting the total phenolics and HPLC peak 2 components while sonicator/vortexing was more efficient in extracting peak 1 component.
Key words: Solvent, extraction, method, musk tree, phenolic. INTRODUCTION
Polyphenols are a large family of metabolic compounds that occur naturally in a wide variety of plant foods (Milner 1994; Luthria and Mukhopadyhyay, 2006). Recently, polyphenols have attracted a great deal of attention due to their roles as natural antioxidants. Antioxidants are believed to provide protective effects against oxidative damage diseases such as cancer, coronary heart disease and stroke. In fact epidemiological, clinical and laboratory studies suggest that consumption of fruits, vegetables and herbs containing polyphenols reduce the risk of chronic diseases such as coronary heart disease and cancer (Hertog, 1995; Rice-Evans et al., 1996; Middleton et al., 2000; Robbins 2003; Wang, 2013). According to Milner (1994) approximately 90% of all cancer cases correlate with environmental factors, including one’s dietary habits, and one-third of all cancer
Africana, P (2021). Development of extraction protocol for phenolic compounds in musk tree seed (Buchholzia coriacea). Afribary.com: Retrieved March 01, 2021, from https://afribary.com/works/development-of-extraction-protocol-for-phenolic-compounds-in-musk-tree-seed-buchholzia-coriacea
Public Library, Africana. "Development of extraction protocol for phenolic compounds in musk tree seed (Buchholzia coriacea)" Afribary.com. Afribary.com, 23 Feb. 2021, https://afribary.com/works/development-of-extraction-protocol-for-phenolic-compounds-in-musk-tree-seed-buchholzia-coriacea . Accessed 01 Mar. 2021.
Public Library, Africana. "Development of extraction protocol for phenolic compounds in musk tree seed (Buchholzia coriacea)". Afribary.com, Afribary.com, 23 Feb. 2021. Web. 01 Mar. 2021. < https://afribary.com/works/development-of-extraction-protocol-for-phenolic-compounds-in-musk-tree-seed-buchholzia-coriacea >.
Public Library, Africana. "Development of extraction protocol for phenolic compounds in musk tree seed (Buchholzia coriacea)" Afribary.com (2021). Accessed March 01, 2021. https://afribary.com/works/development-of-extraction-protocol-for-phenolic-compounds-in-musk-tree-seed-buchholzia-coriacea