Abstract
Objectives: Echinococcosis is a cyclozoonotic disease caused by E. granulosus. The canine mainly the dog represents the definitive host and immunity plays an important role in the control of the disease. This study was aimed to detect Echinococcus granulosus B-antibody for Dot-ELISA and antigen for counter-current immune electrophoresis (CIEP) and bacterial co-agglutination assay (Co-A).
Methods: Follow-up of 23 surgical and chemotherapy patients from different hospitals of Khartoum were performed.
Results: Results: Sensitivity and specificity for Dot-ELISA was 94.4% and 100%, respectively within seven days to 6 months except for the relapse all or most of the cases were negative for the disease whereas the sensitivity of CIEP and Co-A for Echinococcus granulosus antigen in patient sera was 77.8% and 94.4% respectively with a specificity of 100% and 75% respectively. Dot-ELISA, showed high positive and negative predictive value (100%, 95.2%), respectively with CIEP (100%, 83.3%) and in Co-A (81%, 94.1%).
Conclusion: Conclusion: Therefore, detection of antigen of E. granulosus in the serum of operative, post-operative, or chemotherapeutic treated patient using CIEP and Co-A or detection of antibodies using IgG ELISA to the antigen Brich fraction in (ELISA) assay is useful for following treated hydatid patients.
Kahwa, I., Siddig, O , Ali, M , Almahadi, I & Bashir, M (2021). Diagnosis of Pre- and Post-treatment of Echinococcus granulosus with Counter current Immune Electrophoresis and Bacterial Co-agglutination. Afribary. Retrieved from https://afribary.com/works/diagnosis-of-pre-and-post-treatment-of-echinococcus-granulosus-with-counter-current-immune-electrophoresis-and-bacterial-co-agglutination
Kahwa, Ivan, et. al. "Diagnosis of Pre- and Post-treatment of Echinococcus granulosus with Counter current Immune Electrophoresis and Bacterial Co-agglutination" Afribary. Afribary, 28 Jul. 2021, https://afribary.com/works/diagnosis-of-pre-and-post-treatment-of-echinococcus-granulosus-with-counter-current-immune-electrophoresis-and-bacterial-co-agglutination. Accessed 26 Apr. 2024.
Kahwa, Ivan, Omer Siddig , Mai Ali , Ibrahim Almahadi and Mona Bashir . "Diagnosis of Pre- and Post-treatment of Echinococcus granulosus with Counter current Immune Electrophoresis and Bacterial Co-agglutination". Afribary, Afribary, 28 Jul. 2021. Web. 26 Apr. 2024. < https://afribary.com/works/diagnosis-of-pre-and-post-treatment-of-echinococcus-granulosus-with-counter-current-immune-electrophoresis-and-bacterial-co-agglutination >.
Kahwa, Ivan, Omer Siddig , Mai Ali , Ibrahim Almahadi and Mona Bashir . "Diagnosis of Pre- and Post-treatment of Echinococcus granulosus with Counter current Immune Electrophoresis and Bacterial Co-agglutination" Afribary (2021). Accessed April 26, 2024. https://afribary.com/works/diagnosis-of-pre-and-post-treatment-of-echinococcus-granulosus-with-counter-current-immune-electrophoresis-and-bacterial-co-agglutination