Extracts of two indigenous Ghanaian medicinal plants Clausena anisata and Hyptis spicigera
were bioassayed against housefly (Musca domestica L.) and cockroach (Periplaneta
americana (L)) for various insect control activity. The extractives bioassayed were the
petroleum ether and methanol extracts and essential oil of the plants. The lethal toxicity of the
plant extractives were bioassayed using topical application and petri dish residual method
against adult flies and cockroach nymphs respectively. The essential oils exhibited knockdown
effect when they were bioassayed against housefly by topical application. A treated filter
paper method was used to evaluate the repellency of the extractives against housefly. The
crude extracts were also bioassayed for growth regulatory effect against the second larval instar
of housefly using a treated larval breeding medium. A treated rearing medium method was
also used to test the antifeedant effect of the crude extracts against cockroaches.
Per cent mortality, knockdown and index of repellency induced by the extractives against the
insects were generally dose-dependent. The essential oil of the two plants were however more
effective as toxicants and repellents than the crude extracts. The LD 50 of the essential oil of
C. anisata and// spicigera were 2.2101 and 0.8580 //g respectively, against flies and 0.0096
and 0.0310 /ig cm' 2 respectively against cockroach nymphs. The LD50 of the solvent
extractives ranged between 0.4143 and 1746.2 mg for housefly and 0.6869 to 2.600 mg cm"2
for cockroach The knockdown potency of essential oils from H. spicigera (KD 50= 0.2631)
was better than for C. anisata (KD50 — 0.7445). The RD50 of C. anisata and H. spicigera
in 30 min were 0.0400 and 0.0509 //g cm"2 against housefly. The RD 50 of the crude extracts
ranged from 0.1288 to 1.3768 mg cm'2 against housefly. The crude extracts however
demonstrated poor growth regulatory and antifeedant effects.
Eastern Network, L (2021). EFFECTS OF EXTRACTS AND HYDRODISTILLATES OF Clausena Anisata (Wild.) Ex. Benth. AND Hyptis Spicigera Lam. ON Musca Domestica L. AND Periplaneta Americana (L. Afribary.com: Retrieved April 11, 2021, from https://afribary.com/works/effects-of-extracts-and-hydrodistillates-of-clausena-anisata-wild-ex-benth-and-hyptis-spicigera-lam-on-musca-domestica-l-and-periplaneta-americana-l
Library, Eastern Network. "EFFECTS OF EXTRACTS AND HYDRODISTILLATES OF Clausena Anisata (Wild.) Ex. Benth. AND Hyptis Spicigera Lam. ON Musca Domestica L. AND Periplaneta Americana (L" Afribary.com. Afribary.com, 05 Apr. 2021, https://afribary.com/works/effects-of-extracts-and-hydrodistillates-of-clausena-anisata-wild-ex-benth-and-hyptis-spicigera-lam-on-musca-domestica-l-and-periplaneta-americana-l . Accessed 11 Apr. 2021.
Library, Eastern Network. "EFFECTS OF EXTRACTS AND HYDRODISTILLATES OF Clausena Anisata (Wild.) Ex. Benth. AND Hyptis Spicigera Lam. ON Musca Domestica L. AND Periplaneta Americana (L". Afribary.com, Afribary.com, 05 Apr. 2021. Web. 11 Apr. 2021. < https://afribary.com/works/effects-of-extracts-and-hydrodistillates-of-clausena-anisata-wild-ex-benth-and-hyptis-spicigera-lam-on-musca-domestica-l-and-periplaneta-americana-l >.
Library, Eastern Network. "EFFECTS OF EXTRACTS AND HYDRODISTILLATES OF Clausena Anisata (Wild.) Ex. Benth. AND Hyptis Spicigera Lam. ON Musca Domestica L. AND Periplaneta Americana (L" Afribary.com (2021). Accessed April 11, 2021. https://afribary.com/works/effects-of-extracts-and-hydrodistillates-of-clausena-anisata-wild-ex-benth-and-hyptis-spicigera-lam-on-musca-domestica-l-and-periplaneta-americana-l