Abstract
In Namibia seawater is employed by marine fish factories during processing. Previous
studies indicated that chlorine and Ultra Violet (UV) light are ineffective in keeping
seawater free from bacteria throughout the distribution system. However, laboratory
studies showed that hydrogen peroxide eliminates both sessile and attached bacteria
from seawater. Secondly it is not known whether Vibrio alginolyticus bacteria isolated
from the Namibian marine waters and from hake fish whose detection results into
rejection of export products are pathogenic or not. This study was aimed at validating
the ability of hydrogen peroxide (H2O2) to eliminate V. alginolyticus in seawater and to
detect Vibrio species associated virulence factors in V. alginolyticus strains isolated from
seawater and from hake fish products. Seawater used by factory B was dosed with
0.05% H2O2 and allowed to pass through pipes to collection points after which Vibrio
counts in water and in fish products were determined. Secondly, V. alginolyticus strains
isolated from seawater and hake fish and identified using 16S rRNA and rpoB genes
were screened for toxR, tlh and tdh genes using PCR. V. parahaemolyticus LMG 2850
and V. alginolyticus Bcc892 were used as positive and negative controls respectively.
High bacterial counts were detected from seawater treated with chlorine alone. Vibrio
alginolyticus was detected in the fish processing factories were seawater was used and
on fish products. Vibrio species were eliminated from seawater distribution system by
0.05% H2O2. Only one (1.9 %) out of 54 strains, tested tlh positive, but all the strains
tested were toxR and tdh negative. This study, confirmed that these strains do not
contain specific virulence genes known in other Vibrio species. Furthermore, this study
confirmed that H2O2 is an effective disinfectant of seawater.
Shetunyenga, D (2021). Investigation Of The Pathogenicity Of Vibrio Alginolyticus Strains Isolated From Processed Hake And Seawater In Namibia And Validation Of Hydrogen Peroxide (H2o2) For The Disinfection Of Seaw. Afribary. Retrieved from https://afribary.com/works/investigation-of-the-pathogenicity-of-vibrio-alginolyticus-strains-isolated-from-processed-hake-and-seawater-in-namibia-and-validation-of-hydrogen-peroxide-h2o2-for-the-disinfection-of-seawat
Shetunyenga, Dionisius "Investigation Of The Pathogenicity Of Vibrio Alginolyticus Strains Isolated From Processed Hake And Seawater In Namibia And Validation Of Hydrogen Peroxide (H2o2) For The Disinfection Of Seaw" Afribary. Afribary, 25 Apr. 2021, https://afribary.com/works/investigation-of-the-pathogenicity-of-vibrio-alginolyticus-strains-isolated-from-processed-hake-and-seawater-in-namibia-and-validation-of-hydrogen-peroxide-h2o2-for-the-disinfection-of-seawat. Accessed 24 Apr. 2024.
Shetunyenga, Dionisius . "Investigation Of The Pathogenicity Of Vibrio Alginolyticus Strains Isolated From Processed Hake And Seawater In Namibia And Validation Of Hydrogen Peroxide (H2o2) For The Disinfection Of Seaw". Afribary, Afribary, 25 Apr. 2021. Web. 24 Apr. 2024. < https://afribary.com/works/investigation-of-the-pathogenicity-of-vibrio-alginolyticus-strains-isolated-from-processed-hake-and-seawater-in-namibia-and-validation-of-hydrogen-peroxide-h2o2-for-the-disinfection-of-seawat >.
Shetunyenga, Dionisius . "Investigation Of The Pathogenicity Of Vibrio Alginolyticus Strains Isolated From Processed Hake And Seawater In Namibia And Validation Of Hydrogen Peroxide (H2o2) For The Disinfection Of Seaw" Afribary (2021). Accessed April 24, 2024. https://afribary.com/works/investigation-of-the-pathogenicity-of-vibrio-alginolyticus-strains-isolated-from-processed-hake-and-seawater-in-namibia-and-validation-of-hydrogen-peroxide-h2o2-for-the-disinfection-of-seawat