Isolation Serotyping And Molecular Characterization Of Enteric Pathogens For Validation Of “Peepoo” Sanitization Along Handling Chain At Kibera, Nairobi Kenya

An estimated 1.8 billion people in the world use water coming from fecally contaminated sources. This is as a result of 80% of the human generated wastewater ending up in rivers and other reusable water bodies before treatment. The hygienic balance is locally complicated by the fact that 57% of people in informal settlements have no access to basic sanitation, including latrines and toilets. This study sought to evaluate alternative toileting in the name of “Peepoo”, as single use personal toilet for human excreta sanitization and reuse as safe fertilizer, thus preventing dissemination of pathogens to the environment. A cross sectional study was conducted to sample 11 schools enrolled in the project working site in Kibera. Using standard microbiological methods, baseline faecal pathogen concentrations were determined as prevalences at the start of sanitisation and used to validate the “Peepoo” bag along handling chain. Specific primers were used for molecular characterization of isolated pathogens and detection of resistant genes. The prevalence of pathogenic bacteria (Salmonella enterica subspecies arizonae IIIa (0.34%), pathogenic Escherichia coli (7%), Campylobacter spp (18.3%)protozoa (Cryptosporidium spp (2.4%), Entamoeba histolytica (28%), soil transmitted helminth (Ascaris lumblicoides (20%) and Trichuris trichura (13%) eggs and gastrointestinal viruses (Rotavirus (0%) and Adenovirus (1.1%), were determined. There was marked difference in parasitism distribution among schools that was attributed to contamination of school compound with raw sewage.

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APA

JOHNSON, N (2021). Isolation Serotyping And Molecular Characterization Of Enteric Pathogens For Validation Of “Peepoo” Sanitization Along Handling Chain At Kibera, Nairobi Kenya. Afribary. Retrieved from https://afribary.com/works/isolation-serotyping-and-molecular-characterization-of-enteric-pathogens-for-validation-of-peepoo-sanitization-along-handling-chain-at-kibera-nairobi-kenya-1

MLA 8th

JOHNSON, NDUHIU "Isolation Serotyping And Molecular Characterization Of Enteric Pathogens For Validation Of “Peepoo” Sanitization Along Handling Chain At Kibera, Nairobi Kenya" Afribary. Afribary, 05 Jun. 2021, https://afribary.com/works/isolation-serotyping-and-molecular-characterization-of-enteric-pathogens-for-validation-of-peepoo-sanitization-along-handling-chain-at-kibera-nairobi-kenya-1. Accessed 13 Jun. 2024.

MLA7

JOHNSON, NDUHIU . "Isolation Serotyping And Molecular Characterization Of Enteric Pathogens For Validation Of “Peepoo” Sanitization Along Handling Chain At Kibera, Nairobi Kenya". Afribary, Afribary, 05 Jun. 2021. Web. 13 Jun. 2024. < https://afribary.com/works/isolation-serotyping-and-molecular-characterization-of-enteric-pathogens-for-validation-of-peepoo-sanitization-along-handling-chain-at-kibera-nairobi-kenya-1 >.

Chicago

JOHNSON, NDUHIU . "Isolation Serotyping And Molecular Characterization Of Enteric Pathogens For Validation Of “Peepoo” Sanitization Along Handling Chain At Kibera, Nairobi Kenya" Afribary (2021). Accessed June 13, 2024. https://afribary.com/works/isolation-serotyping-and-molecular-characterization-of-enteric-pathogens-for-validation-of-peepoo-sanitization-along-handling-chain-at-kibera-nairobi-kenya-1