Hepatitis B virus (HBV) infection is a major cause of liver inflammation accounting
for 887,000 deaths annually. The introduction of HBV vaccines has significantly
reduced new cases of the infection, but there are still 65 million people living with the
virus in Sub-Saharan Africa. Currently, 10 genotypes (A-J) of HBV have been
classified. Several studies have shown that the different HBV genotypes differently
influence clinical presentation, progression of liver diseases and response to treatment.
In Ghana, studies on HBV circulating genotypes and their implications on
epidemiology is limited, however few available data have been obtained mostly in the
urban areas of the Ashanti and Greater Accra regions. This study is aimed at
characterizing HBV genotypes in patients attending three district hospitals in the
Central Region of Ghana (Agona-West, Effutu Municipal and Gomoa-West district
hospitals). Questionnaires were administered to the patients before sample collection
which included their gender, age, educational levels and risk factors. There was no
association between socio-demographic factors and HBV infectivity and
transmissibility, except educational levels. HBV DNA was extracted from the blood of
173 HBsAg seropositive patients using QIAamp DNA Blood mini kit; 70 (40%)
samples from the Gomoa-West district, 50 (29%) samples from the Effutu Municipal
and 53 (31%) from the Agona-West district. The extracted DNA was confirmed by
amplifying the S region of the HBV genome using conventional PCR. A total of 115
(66.5%) patients were found to be PCR positive, with 58 (33.5%) negative for HBV
DNA. The HBV was genotyped by nested-multiplex PCR using type-specific primers
that amplifies the preS/S region of the HBV genome. To confirm the specificity of the
nested-multiplex PCR assay, 31 of the samples obtained from the S gene amplification
were sent for Sanger sequencing in one direction. The nucleotide sequences obtained
were submitted into the HBV HepSeq genotyping software to confirm the genotypes.
The genotype results obtained from both the software and the nested -multiplex PCR
assay included; 67.1% genotype E which was the most dominant genotype, followed
by genotypes A, D and G with 1.7%, 0.6% and 0.6%, respectively. The phylogenetic
analysis of the sequenced isolates from the three districts clustered together with the
reference strains. The characterization of the HBV genotypes will help understand the
transmission dynamics of the virus in the rural and semi-urban setting.
Eastern Network, L (2021). Molecular Characterization Of Hepatitis B Virus In Rural And Semi-Urban Areas In The Central Region Of Ghana. Afribary.com: Retrieved April 15, 2021, from https://afribary.com/works/molecular-characterization-of-hepatitis-b-virus-in-rural-and-semi-urban-areas-in-the-central-region-of-ghana
Library, Eastern Network. "Molecular Characterization Of Hepatitis B Virus In Rural And Semi-Urban Areas In The Central Region Of Ghana" Afribary.com. Afribary.com, 05 Apr. 2021, https://afribary.com/works/molecular-characterization-of-hepatitis-b-virus-in-rural-and-semi-urban-areas-in-the-central-region-of-ghana . Accessed 15 Apr. 2021.
Library, Eastern Network. "Molecular Characterization Of Hepatitis B Virus In Rural And Semi-Urban Areas In The Central Region Of Ghana". Afribary.com, Afribary.com, 05 Apr. 2021. Web. 15 Apr. 2021. < https://afribary.com/works/molecular-characterization-of-hepatitis-b-virus-in-rural-and-semi-urban-areas-in-the-central-region-of-ghana >.
Library, Eastern Network. "Molecular Characterization Of Hepatitis B Virus In Rural And Semi-Urban Areas In The Central Region Of Ghana" Afribary.com (2021). Accessed April 15, 2021. https://afribary.com/works/molecular-characterization-of-hepatitis-b-virus-in-rural-and-semi-urban-areas-in-the-central-region-of-ghana