Abstract/Overview
Background Multiplex cytometric bead assay (CBA) have a number of advantages over ELISA for antibody testing, but little information is available on standardization and validation of antibody CBA to multiple Plasmodium falciparum antigens. The present study was set to determine optimal parameters for multiplex testing of antibodies to P. falciparum antigens, and to compare results of multiplex CBA to ELISA. Methods Antibodies to ten recombinant P. falciparum antigens were measured by CBA and ELISA in samples from 30 individuals from a malaria endemic area of Kenya and compared to known positive and negative control plasma samples. Optimal antigen amounts, monoplex vs multiplex testing, plasma dilution, optimal buffer, number of beads required were assessed for CBA testing, and results from CBA vs. ELISA testing were compared. Results Optimal amounts for CBA antibody testing differed according to antigen. Results for monoplex CBA testing correlated strongly with multiplex testing for all antigens (r = 0.88-0.99, P values from
N., O (2024). Standardization and validation of a cytometric bead assay to assess antibodies to multiple Plasmodium falciparum recombinant antigens. Afribary. Retrieved from https://afribary.com/works/standardization-and-validation-of-a-cytometric-bead-assay-to-assess-antibodies-to-multiple-plasmodium-falciparum-recombinant-antigens
N., Ondigo "Standardization and validation of a cytometric bead assay to assess antibodies to multiple Plasmodium falciparum recombinant antigens" Afribary. Afribary, 16 Jul. 2024, https://afribary.com/works/standardization-and-validation-of-a-cytometric-bead-assay-to-assess-antibodies-to-multiple-plasmodium-falciparum-recombinant-antigens. Accessed 23 Nov. 2024.
N., Ondigo . "Standardization and validation of a cytometric bead assay to assess antibodies to multiple Plasmodium falciparum recombinant antigens". Afribary, Afribary, 16 Jul. 2024. Web. 23 Nov. 2024. < https://afribary.com/works/standardization-and-validation-of-a-cytometric-bead-assay-to-assess-antibodies-to-multiple-plasmodium-falciparum-recombinant-antigens >.
N., Ondigo . "Standardization and validation of a cytometric bead assay to assess antibodies to multiple Plasmodium falciparum recombinant antigens" Afribary (2024). Accessed November 23, 2024. https://afribary.com/works/standardization-and-validation-of-a-cytometric-bead-assay-to-assess-antibodies-to-multiple-plasmodium-falciparum-recombinant-antigens