ABSTRACT Vernonia amygdalina leaves, commonly called bitter leaf is widely consumed in Africa especially in Nigeria. The leaf extract has been reported to have prebiotic potentials but limited information is available on the immune-modulatory effects. This study evaluated the immuno-modulatory properties of the leaf extract and identified the prebiotic components. The aqueous extract was separated into fractions by Liquid-liquid fractionation and the prebiotic compounds were identified th...