Contraceptive And Morphometric Effects Of The Aqueous Extract Of Carica Papaya Bark On Male Sprague Dawley Rats

ABSTRACT

The overall aim of this research is to examine if the bark extract of Carica papaya would serve as a male contraceptive agent by determining the morphological response of the testes of Sprague-Dawley rats using histological and stereological parameters.

Methodology: 60 adult (6-8 weeks old ) male Sprague-Dawley rats were used for this experiment which was conducted in 3 (three ) groups of 4 weeks, 8 weeks and the reversal group. In each group, rats were subdivided into another group of three. Group 1 served as the control group, while groups 11 and 111 were fed with low (50mg/ml/day) and high (100mg/ml/day) doses of the extract respectively. Histological assessment and stereological measurements were taken for both 4 weeks and 8 weeks groups at the end of the experiment while the reversibility effect was also assessed after 8 weeks reversal period.

Tissues were processed for histological examination under light microscopy, immunological assay and stereological analysis. Stereological parameters estimated include : Tubular diameter, cross sectional area of seminiferous tubules , volume density, number of profiles per unit area, absolute volume of seminiferous tubules and testicular intersticium, numerical density, length density and star volume of the seminiferous tubules.

There was also degeneration of the seminiferous tubules following a short term administration of the extract at a dose of 50mg/ml/day and a destruction of the tubules and testicular intersticium following a long term administration of the extract at a dose of 100mg/ml/day.