Contraceptive Effect Of Methanolic Seed Extract Of Momordica Charantia On Male Sprague-Dawley Rats

ABSTRACT

The research work investigated the possible antifertility effect of the methanolic seed extract of Momordica charantia (Mc). The morphological changes following administration of the extract on testes of Sprague-Dawley rats (SD-rats) were assessed using stereological parameters, hormonal assay and measurement of testicular 'stress indicators

Methodology: 670 adult male SD rats weighing 162452 g were used for the study, which was carried out in two phases of 40 weeks. There were 335 adult male rats used in each phase. The two phases of the experiments were the same, the second one separated from the first by a time span of 4 weeks. In each phase, the male SD-rats were randomly divided into 6 main groups (A - F) and 36 sub-groups comprising 5-10 experimental rats. The animals were treated orally for short, intermediate and long term durations with Mc seed extract at dose of 50 mg/100g body weight. The recovery effect was assessed at the end of 8 weeks of administration to check for its reversibility. This was done 8 weekly until the 40" week. The prophylactic, modulating and post-exposure therapeutic effects of Testosterone, Vitamins and Eon effect of the extract were assessed. The animals were weighed weekly and sacrificed at the end of various durations between weeks 2 and 40. The harvested testes were processed for histological examination under light microscopy and subjected to stereological analysis. Stereological parameters estimated include: Tubular diameter, cross sectional area of seminiferous tubules, number of profiles per unit area, length density and numerical density of the seminiferous tubules.