DISTRIBUTION OF ABO, Rh (RHESUS) BLOOD GROUPING AND HEPATITIS B AMONG BLOOD DONORS WITH NATIONAL BLOOD TRANSFUSION SERVICE KADUNA

ABSTRACT

ABO and Rh blood group systems are of major clinical significance amongst the thirty (30) human blood group system. The Distribution of ABO, Rh blood grouping and hepatitis B virus among blood donors with National Blood Transfusion Service, Kaduna was carried out. Two hundred voluntary blood donors (200) were used. Questionnaire was used to obtain the respondents’ bio-demographic data. Microplate technique was used for ABO and Rh blood grouping tests and ELISA kit (Monolisa HBsAg ULTRA) for hepatitis B virus test. The results showed that blood group O donors had (47.0%, group A (31.5%), group B (17.0%) and group AB (4.5%).  Rh D positive has (95.0%) and RhD negative (5%).  Reactive hepatitis B (12.5% and non-reactive hepatitis B (87.5%).

CHAPTER ONE

1.0Introduction

1.1Aim of the Study

1.2Objectives of the Study

1.3Justification of Study

CHAPTER TWO

2.0REVIEW OF LITERATURE

2.1Introduction 

2.1.1Screening

2.2ABO Blood Groups System 

2.2.1ABO blood group genetics 

2.2.2Anti-A and anti-B antibodies 

2.2.3ABO subgroups 

2.3Rhesus (Rh) grouping system

2.3.1Rh Antigens 

2.3.2Rh Antibodies 

2.3.3Weak Antigen D

2.3.4Importance of Rh D Grouping

2.4Hepatitis B Virus (HBV)

2.5Hepatitis B Surface Antigen 

2.6Hepatitis B Core Antibody 

2.7Hepatitis B Virus DNA

2.8Microplate technique grouping

2.9Principle of the monolisaTM HBsAg ULTRA  

2.10Interpretation

2.11Interpretation for Monolisa HBsAg ULTRA

                      

CHAPTER THREE

3.0MATERIALS AND METHOD

CHAPTER FOUR

RESULTS

4.1DISCUSSION

CHAPTER FIVE

CONCLUSION, RECOMMENDATIONS AND LIMITATIONS

5.1CONCLUSION

5.2RECOMMENDATIONS

5.3LIMITATIONS