Evaluation of Homocysteine level and some Hemotological Parameters in End Stage Renal Failure Patients

Abstract

Background: Chronic Renal Failure is a major health problem and affects the economic and social status of patients. In Sudan, according to ministry of health records, the prevalence of renal failure is increasing approximately 70 to 140 new patients undergo dialysis each year. Although Homocystin ratio has been strongly linked to end stage renal disease, biochemical test is restricted to monitoring kidney function. Therefore, introducing homocysteine as a biomarker of ESRD in Sudan hospitals is recommended.

Objective: To assess homocysteine and hematological indices in hemodialysis patients at. Sudanese Kidney Transplanted Association.

Methods: This case-control study was conducted in Sudanese Kidney Transplanted Association 2018, compared plasma homcysteine and some hemoatological parameters in 30 renal failure patients as case group and 30 helathy Sudanese people at matched age as a control group. data of pations was collected from record saved in the computer

Automated hematological analyzer (Sysmex 21N KX-) was used measure some hematological parameters (HB, RBCs, PCV, MCV, MCH, MCHC, TWBC, platelates). An auto,ated chemistry analyzer (DRIRUS, CS-T240) used to measure the plasma hemocysteine level.

Results were analyzed using statistical package for social science (SPSS version 16) computer program. Independent T test was used for data analysis and person's correlation test was used for correlation.

Results: The Study show that that there was significant decrease in mean of HCY (μmol/L), HB(g/dl), RBCs(c/ μL), PCV(%), Platelets (L) in case group when compared with control (8.3 versus 10.8 p-value 0.000), (9.5 versus 12.3, P-value 0.000), ( 3.6 versus 4.9, P-value 0.000), (30.1 versus 40.9, P-value 0.000), (218 versus 301, P-value 0.000) respectively.

while the mean level of MCV (ft), MCH(pg),MCHC(%), TWBC(Cmm) exhibited insifnificant defference in case compared with control (83.1 versus 86.0, P-value 0.125),(26.0 versus 25.7, P-value 0.643), 30.8 versus 30.4, P-value 0.433),(14.2 versus 6.6 P-value 0.230) respectively.

There was correlation between value of homocysteine and HB, RBCs, PCV, Platelets (r= 0.276 probability value 0.033), (r = 0.332 probability value 0.010), ( r = 0.332 probability value 0.010) respectively.

While there was no correlation between plasma homocysteine and MCV,MCH, MCHC, TWB Count ( r - 0.062 probability value 0.637) , (r = 0.134 probability value 0.545) respectively

Conclusions: homocysteine, hemoglobin, red blood cells, hematocrite and platelets was significantly decrease and there was non significant different in mean cell volume, mean cell Hemoglobin, mean cell Hemoglobin concentration, total white blood cel in renal failure paients. Homocysteine was positively correlated with hemoglobin, red blood cells, hematocrite and platelets. There is no relation between homocysteine and other hematological parameter.