Biotechnology Research Papers/Topics

HYBIDIZATION STUDIES IN OKRA (Abelmoschus spp (L.) Moench)

ABSTRACT Okra (Abelmoschus spp. L. Moench) is an important multi-purpose vegetable crop cultivated and consumed across all tropical and temperate regions of the world. In Ghana,it is popular in all ten regions and increasing quantities are exported to Europe in the fresh form. The crop has received little attention by way of breeding to produce varieties combining the most desirable qualities to boost local cultivation and export. Ten accessions of Abelmoschus spp., comprising two species...

Genetic diversity of Plasmodium falciparum isolates from naturally infected children in north-central Nigeria using the merozoite surface protein-2 as molecular marker

ABSTRACT Objective: To characterize the genetic diversity of Plasmodium falciparum (P. falciparum) field isolates in children from Lafia, North-central Nigeria, using the highly polymorphic P. falciparum merozoite surface protein 2 (MSP-2) gene as molecular marker. Methods: Three hundred and twenty children were enrolled into the study between 2005 and 2006. These included 140 children who presented with uncomplicated malaria at the Dalhatu Araf Specialist Hospital, Lafia and another 180 chil...

PHYTOCHEMISTRY, ANTI-INFLAMMATORY AND ANTIOXIDANT ACTIVITIES OF THE ROOT BARK OF ANTHOSTEMA AUBRYANUM (BAILL)

ABSTRACT The work presented in this thesis involves the scientific investigation into the traditional uses of the root bark of Anthostema aubryanum (Baill., family, Euphorbiaceae) as an anti-inflammatory and antioxidant agent. It also describes the isolation and characterization of two compounds from the alkaloid extract of the root bark of Anthostema aubryanum Baill. The anti-inflammatory activity was investigated using the acute carrageenan – induced foot pad edema model in six weeks old ...

ANTIBIOTIC RESISTANCE, PHYLOGENETIC GROUPING AND VIRULENCE POTENTIAL OF ESCHERICHIA COLI ISOLATED FROM CLINICAL AND ENVIRONMENTAL SAMPLES FROM THE CAPE COAST METROPOLIS OF THE CENTRAL REGION

ABSTRACT The genetic diversity of Escherichia coli isolated from clinical and environmental samples from Cape Coast was studied. Bacterial isolation and identification was carried out using standard biochemical tests and confirmed using Analytical Profile Index (API) 20 E kits. All test E. coli isolates were screened for their susceptibility to 16 antibiotics, followed by a screening for extended spectrum β-lactamase (ESBL) production. DNA was extracted from all tests E. coli isolates and PC...

Testicular and Hepatic Toxicity of Monosodium Glutamate on Adult Wistar Rats

ABSTRACT Monosodium Glutamate is a widely used food additive and flavor enhancer that is present in most soups, salads and processed meat and also present in packaged food without appearing on the label. This could result to inadvertent consumption of monosodium glutamate in high concentrations. The present study investigated the effect of monosodium glutamate on liver and testes of adult male wistar rats, by daily oral exposure of different doses. Wistar rats (n=20) of the average weight of ...

Determination of Occurence of Nitrifying Bacteria in a Freshwater Fishpond of Circulatory Aquaponics System

ABSTRACT Nitrification is the biological oxidation of ammonia into nitrite, followed by the oxidation of nitrite into nitrate by small groups of autotrophic bacteria and Achaea. NH3 removal is beneficial to the plant system as build up is dangerous. Presence and activities of ammonia oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB) in freshwater fishpond of a circulating aquaponics system was examined in respect to random points in the aquaponics system (the fish tank, the bio-fi...

Production of Bioethanol from Molasses and Effluent Treatment of Spent Wash

ABSTRACT The use of ethanol in fuel blending has long been in use, and this help in controlling gas emissions but cannot be compared to bio-ethanol in control of greenhouse gas emissions. Bio-ethanol is known to adsorb CO2 they emit; it also improves rural economy. A lot of materials especially starchy crops are used in the production of bio-ethanol. This research work focuses on the production of bio-ethanol from molasses; a waste from sugar cane industries. Bio-ethanol is regarded as one of...

Study of Oxidative Stress Induced by Cassava Effluent on Juvenile Clarias Gariepinus Using Antioxidant Enzyme System

ABSTRACT Aquatic environmental stress of cassava effluent on catfish Juvenile (carias gariepinus) of about 5 months, 8 10cm in length and mean weight of 3.42 0.2g was investigated using antioxidant enzymes system. A total 125 Juvenile clarias gariepinus were used for the study which was further divided into five treatment groups of 25 each. Thus control, group I, group II, group III and group IV. Each of these groups were exposed to 0.1%, 0.3%, 0.5% and 1% of cassava effluents respectivel...


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