ABSTRACT
Rapid and accurate diagnosis is paramount in understanding the infection status of foot
and-mouth disease (FMD) virus (FMDV) in animals. In this study, the singleplex real
time RT-PCR (qRT-PCR) assay employing the Callahan 3DF-2, 3DF-R primers and
Callahan 3DP-1 probe were used in screening for FMDV genome on esophageal–
pharyngeal (OP) fluids. The OP samples were collected from cattle and African buffaloes
in livestock-wildlife interface areas of Mikumi, Mkomazi and Ruaha National Parks in
Tanzania in 2011, which included National Parks and surrounding areas. The detection
rates of FMDV genome were 5.88% (n = 3), 19.44% (n = 7) and 41.18% (n = 21) in
Mkomazi, Ruaha and Mikumi National Parks, respectively. FMDV detection rates in
Mkomazi and Mikumi were significantly higher in the African buffaloes (p < 0.05)
compared to that in cattle. There was no correlation of FMDV detection with either age or
sex of the animals in the three National Parks. These findings indicate that cattle and
buffaloes in Mikumi, Ruaha and Mkomazi were naturally infected with FMDV.
Furthermore, the higher FMDV detection rates in buffaloes suggest that buffaloes could
potentially act as reservoirs for FMDV and possibly play a significant role in transmission
of the virus to other in-contact susceptible animals. Further studies, including serotyping,
virus isolation, experimental infection and sequencing of the viruses, are required to
elucidate the complex epidemiology of FMD in cattle and buffaloes in the livestockwildlife interface areas in Tanzania.
PETER, E (2021). Screening For Foot And Mouth Disease Virus In Buffaloes And Cattle In Selected Livestock-Wildlife Interface Areas Of Tanzania. Afribary. Retrieved from https://afribary.com/works/screening-for-foot-and-mouth-disease-virus-in-buffaloes-and-cattle-in-selected-livestock-wildlife-interface-areas-of-tanzania
PETER, EMMA "Screening For Foot And Mouth Disease Virus In Buffaloes And Cattle In Selected Livestock-Wildlife Interface Areas Of Tanzania" Afribary. Afribary, 11 May. 2021, https://afribary.com/works/screening-for-foot-and-mouth-disease-virus-in-buffaloes-and-cattle-in-selected-livestock-wildlife-interface-areas-of-tanzania. Accessed 15 Aug. 2022.
PETER, EMMA . "Screening For Foot And Mouth Disease Virus In Buffaloes And Cattle In Selected Livestock-Wildlife Interface Areas Of Tanzania". Afribary, Afribary, 11 May. 2021. Web. 15 Aug. 2022. < https://afribary.com/works/screening-for-foot-and-mouth-disease-virus-in-buffaloes-and-cattle-in-selected-livestock-wildlife-interface-areas-of-tanzania >.
PETER, EMMA . "Screening For Foot And Mouth Disease Virus In Buffaloes And Cattle In Selected Livestock-Wildlife Interface Areas Of Tanzania" Afribary (2021). Accessed August 15, 2022. https://afribary.com/works/screening-for-foot-and-mouth-disease-virus-in-buffaloes-and-cattle-in-selected-livestock-wildlife-interface-areas-of-tanzania