ABSTRACT
This experiment is designed to investigate the effects of Kigelia africana fruit extract (KAFE) on cisplatin-induced testicular damage in Sprague-Dawley rats. 280 male Sprague-Dawley rats weighing 200 + 20 g were used for the experiments which were run in replicates of 140 rats per set for the purpose of reproducibility of results. The experiments were categorised into short term (28 days) and long term durations (56 days). Mature and ripe fruit of Kigelia africana were obtained and screened phytochemically for the active components. KAFE was administered in sub-lethal doses of 100 mg/kg and 500 mg/kg body weight of rats orally while cisplatin was given intraperitoneally (ip) at a dose of 10 mg/kg. Control animals received an equivalent amount of normal saline. KAFE was administered alone; as prophylaxis; as adjunct and post-treatment to cisplatin injection. All the animals were sacrificed at the end of each phase by i.p injection of ketamine. Each phase of the experiment was divided into ten groups of five rats per group. Acute toxicity studies were carried out to determine the LDs of KAFE. Seminal parameters of sperm count and motility were estimated in epididymal fluid according to World Health Organisation protocols. Testicular tissue was processed for histological analysis using Olympus light microscope, the assay for catalase activity, malondialdehyde and glutathione levels were determined.
OKPARA, A (2021). The Effect Of Kigelia Africana Fruit Extract On Cisplatin-Induced Testicular Damage In Sprague-Dawley Rats. Afribary. Retrieved from https://afribary.com/works/the-effect-of-kigelia-africana-fruit-extract-on-cisplatin-induced-testicular-damage-in-sprague-dawley-rats
OKPARA, AZU "The Effect Of Kigelia Africana Fruit Extract On Cisplatin-Induced Testicular Damage In Sprague-Dawley Rats" Afribary. Afribary, 06 May. 2021, https://afribary.com/works/the-effect-of-kigelia-africana-fruit-extract-on-cisplatin-induced-testicular-damage-in-sprague-dawley-rats. Accessed 22 Nov. 2024.
OKPARA, AZU . "The Effect Of Kigelia Africana Fruit Extract On Cisplatin-Induced Testicular Damage In Sprague-Dawley Rats". Afribary, Afribary, 06 May. 2021. Web. 22 Nov. 2024. < https://afribary.com/works/the-effect-of-kigelia-africana-fruit-extract-on-cisplatin-induced-testicular-damage-in-sprague-dawley-rats >.
OKPARA, AZU . "The Effect Of Kigelia Africana Fruit Extract On Cisplatin-Induced Testicular Damage In Sprague-Dawley Rats" Afribary (2021). Accessed November 22, 2024. https://afribary.com/works/the-effect-of-kigelia-africana-fruit-extract-on-cisplatin-induced-testicular-damage-in-sprague-dawley-rats