AbstractSeeds of Pisum sativum cv. Greenfeast were used in all of
the experiments. In order to prevent imbibitional damage, seeds
were slowly pre-hydrated for 1 hour on damp blotting paper prior
to imbibi tion. In order to ascertain at what stage during
germination the seeds had lost their desiccation-tolerance, seeds
were imbjbed for different intervals (12, 36, 48, 60 hrs) prior
to redrying to their original moisture content at room
temperature (23° C).
SLOW DRYING OF WHOLE SEEDS.
For each imbibitional stage seeds were slowly dried back to
their original moisture content (11. 3 g H20/g dry mass) by
placing them evenly spaced in a laminar flow cabinet. Viability
and moisture content were assessed at time intervals o, 4, 10,
24, 34, 48 hrs by which time the seeds had reached their original
RAPID (FLASH) DRYING OF EMBRYONIC AXES.
Seeds were pre-hydrated as described above. After
imbibition the seeds were removed from the water and the lengths
of their axes were measured as a rough indicator of how far
germination had proceeded. Axes of similar length were selected
for the experiment. The rapid drying of embryonic axes followed
Pammenter et al 's (1991) method: Air was passed at room
temperature (approximately 22° C) through two fish tank air
diffuser• in parallel evenly spaced at the bottom of a plastic
box 10 cm long, 10 cm wide and 4 cm deep. Excised embryonic axes
were placed on fine-mesh nylon supported 2 cm above the air
stones and removed after the required drying time, i.e. 20, 40,
60, 120, 160, 200, 280, 320, 360, 690 minutes until the original
moisture content of the axis was reached. At each time interval,
moisture content (measured as described below for whole seeds)
and viability was assessed as described below.
SSA, R (2021). The Expression of Lea Proteins Pisum Sativum Seeds. Afribary.com: Retrieved May 10, 2021, from https://afribary.com/works/the-expression-of-lea-proteins-pisum-sativum-seeds
Research, SSA. "The Expression of Lea Proteins Pisum Sativum Seeds" Afribary.com. Afribary.com, 24 Apr. 2021, https://afribary.com/works/the-expression-of-lea-proteins-pisum-sativum-seeds . Accessed 10 May. 2021.
Research, SSA. "The Expression of Lea Proteins Pisum Sativum Seeds". Afribary.com, Afribary.com, 24 Apr. 2021. Web. 10 May. 2021. < https://afribary.com/works/the-expression-of-lea-proteins-pisum-sativum-seeds >.
Research, SSA. "The Expression of Lea Proteins Pisum Sativum Seeds" Afribary.com (2021). Accessed May 10, 2021. https://afribary.com/works/the-expression-of-lea-proteins-pisum-sativum-seeds