Abstract
Introduction: Nasopharyngeal carcinoma (NPC) is a malignant epithelial neoplasm arising in the nasopharyngeal mucosa that shows light microscopic and/or ultrastructural evidence of squamous differentiation. Immunohistochemistry (IHC) can be used to reliably distinguish undifferentiated NPC from other malignant tumors, and the technique may be a necessary tool toward the arrival of a definitive diagnosis, particularly when dealing with challenging cases. Materials and Methods: This was a cross‑sectional hospital‑based study which was conducted at Muhimbili National Hospital. The study involved 120 patients with NPC who were diagnosed on histopathological basis between 2009 and 2013. Results: The sensitivity and specificity of hematoxylin and eosin (H and E) stain in diagnosing NPC were 99% and 30.4%, respectively. The accuracy of H and E stain to diagnose NPC and lymphoma was 94.2% and 30.4%, respectively. CD45 antibody helped to confirm 16 cases which were diagnosed as NPC on H and E stain to be lymphoma. Further, AE1/AE3 antibody helped to confirm one case who was diagnosed as rhabdomyosarcoma on H and E stain to be NPC. Conclusions: The sensitivity and accuracy of H and E stains to diagnose NPC were very high whereas the specificity was very low. Asignificant proportion of previously diagnosed NPC cases by routine H and E stains were confirmed not to be so by a minimal IHC antibody panel of pan‑cytokeratin cocktail (AE1/AE3) and leukocyte common antigen (CD45). This highlights the paramount importance of a minimum IHC panel in assisting to obtain a definitive diagnosis in challenging cases of NPC.
Mremi, A , Abraham, Z , Mwakigonja, A & Yahaya, J (2021). The Role of a Minimum Immunohistochemical Antibody Panel in Confirming Undifferentiated Nasopharyngeal Carcinoma: A Cross-Sectional Study at the Muhimbili National Hospital, Dar-es-Salaam, Ta. Afribary. Retrieved from https://afribary.com/works/the-role-of-a-minimum-immunohistochemical-antibody-panel-in-confirming-undifferentiated-nasopharyngeal-carcinoma-a-cross-sectional-study-at-the-muhimbili-national-hospital-dar-es-salaam-tanza
Mremi, Alex et. al. "The Role of a Minimum Immunohistochemical Antibody Panel in Confirming Undifferentiated Nasopharyngeal Carcinoma: A Cross-Sectional Study at the Muhimbili National Hospital, Dar-es-Salaam, Ta" Afribary. Afribary, 01 May. 2021, https://afribary.com/works/the-role-of-a-minimum-immunohistochemical-antibody-panel-in-confirming-undifferentiated-nasopharyngeal-carcinoma-a-cross-sectional-study-at-the-muhimbili-national-hospital-dar-es-salaam-tanza. Accessed 25 Apr. 2024.
Mremi, Alex, Zephania Abraham , Amos Mwakigonja and James Yahaya . "The Role of a Minimum Immunohistochemical Antibody Panel in Confirming Undifferentiated Nasopharyngeal Carcinoma: A Cross-Sectional Study at the Muhimbili National Hospital, Dar-es-Salaam, Ta". Afribary, Afribary, 01 May. 2021. Web. 25 Apr. 2024. < https://afribary.com/works/the-role-of-a-minimum-immunohistochemical-antibody-panel-in-confirming-undifferentiated-nasopharyngeal-carcinoma-a-cross-sectional-study-at-the-muhimbili-national-hospital-dar-es-salaam-tanza >.
Mremi, Alex , Abraham, Zephania , Mwakigonja, Amos and Yahaya, James . "The Role of a Minimum Immunohistochemical Antibody Panel in Confirming Undifferentiated Nasopharyngeal Carcinoma: A Cross-Sectional Study at the Muhimbili National Hospital, Dar-es-Salaam, Ta" Afribary (2021). Accessed April 25, 2024. https://afribary.com/works/the-role-of-a-minimum-immunohistochemical-antibody-panel-in-confirming-undifferentiated-nasopharyngeal-carcinoma-a-cross-sectional-study-at-the-muhimbili-national-hospital-dar-es-salaam-tanza