BACKGROUND: Plasmodium falciparum malaria throughout history has proved to be a significant menace to human health and pregnancy associated malaria (PAM) has been linked to severe consequences in terms of morbidity and mortality. P. falciparum parasites express members of the Plasmodium falciparum Erythrocyte Membrane Protein-1 (PfEMP1) on the surface of infected erythrocytes (IEs) which act as ligands binding to a number of different human vascular host receptors such as Chondroitin Sulphate A (CSA) in the placenta. The adhesion to CSA is mediated by VAR2CSA, which also allows for antigenic variation and immune evasion. VAR2CSA has been considered an important target of acquired protective immunity mediated mainly by specific IgG antibodies. In addition to being targets of specific IgGs, IEs have been demonstrated to select natural IgM in addition to CSA.
AIM: This study was designed to determine if binding of VAR2CSA IEs to nonspecific IgM interferes with specific IgG binding to the VAR2CSA epitopes.
METHOD: Plasma samples from 109 pregnant Ghanaian women of varying gestational age and parity were purified by the Dynabeads immunoprecipitation method for IgG and IgM on Mannan Binding Protein and grouped into three (unpurified plasma containing IgG and IgM (IgG+IgM+), purified IgG from plasma (IgG+IgM-) and purified IgM from same plasma(IgG-IgM+). The levels of antibody reactivity to two different domains of VAR2CSA (DBL1 and DBL5) and two domains of IT4var60 not implicated in placental malaria, but mediates rosetting (DBL1α and DBL5ε) were measured using indirect ELISA.
RESULTS: The study showed increased susceptibility to malaria infection in the primigravidae (13%) than in the multigravidae (2.3%). The levels of IgG measured in the presence of IgM was significantly higher than IgG levels measured in the absence of IgM to both DBL1 domains (p=0.0001). Also there was a significant increase in the reactivity of IgG to the DBL5 domains (VAR2CSA and IT4VAR60) (p
FRIMPONG, A (2021). Var2csa Duffy Binding Like (Dbl) Domains And Non-Specific Igm Binding In The Acquisition Of Acquired Immunity To Pregnancy-Associated Plasmodium Falciparum Malaria. Afribary. Retrieved from https://afribary.com/works/var2csa-duffy-binding-like-dbl-domains-and-non-specific-igm-binding-in-the-acquisition-of-acquired-immunity-to-pregnancy-associated-plasmodium-falciparum-malaria
FRIMPONG, AUGUSTINA "Var2csa Duffy Binding Like (Dbl) Domains And Non-Specific Igm Binding In The Acquisition Of Acquired Immunity To Pregnancy-Associated Plasmodium Falciparum Malaria" Afribary. Afribary, 08 Apr. 2021, https://afribary.com/works/var2csa-duffy-binding-like-dbl-domains-and-non-specific-igm-binding-in-the-acquisition-of-acquired-immunity-to-pregnancy-associated-plasmodium-falciparum-malaria. Accessed 31 Mar. 2023.
FRIMPONG, AUGUSTINA . "Var2csa Duffy Binding Like (Dbl) Domains And Non-Specific Igm Binding In The Acquisition Of Acquired Immunity To Pregnancy-Associated Plasmodium Falciparum Malaria". Afribary, Afribary, 08 Apr. 2021. Web. 31 Mar. 2023. < https://afribary.com/works/var2csa-duffy-binding-like-dbl-domains-and-non-specific-igm-binding-in-the-acquisition-of-acquired-immunity-to-pregnancy-associated-plasmodium-falciparum-malaria >.
FRIMPONG, AUGUSTINA . "Var2csa Duffy Binding Like (Dbl) Domains And Non-Specific Igm Binding In The Acquisition Of Acquired Immunity To Pregnancy-Associated Plasmodium Falciparum Malaria" Afribary (2021). Accessed March 31, 2023. https://afribary.com/works/var2csa-duffy-binding-like-dbl-domains-and-non-specific-igm-binding-in-the-acquisition-of-acquired-immunity-to-pregnancy-associated-plasmodium-falciparum-malaria