Purification and Characterization of Lipase from Aspergillus flavus PW2961 using Magnetic Nanoparticles
________________________________(Received: 08:03:2017; Accepted: 05:04:2017) _______________________ Abstract Lipase from Aspergillus flavus was purified in a single step purification using MnFeO4 magnetic nano particles to achieve a 20.53- fold purification with specific activity of 11.29 U/mg and a 59% recovery yield. SDS-PAGE of lipase showed a single pure band with corresponding molecular weight of 35 kDa. The optimal temperature and pH for the enzyme activity were 45°C and 7.0 respe...