Research Papers/Topics in Applied Microbiology

Extraction of DNA in Tomatoes and Garden Eggs Fruits

ABSTRACT This project is basically concerned with the extraction of DNA in tomatoes and garden egg fruit. An experiment on how to extract DNA on tomatoes and garden egg, isolation on it to see what DNA actually look like as well as importance of DNA extraction are consid3ered in this project. TABLE OF CONTENT Title page ……………………………………………………… i. Certification……………………………………………………..ii De...

Antimicrobial Resistance Pattern of Coagulase-negative Staphylococci isolates from Medical Students of Some Nigerian Teaching Hospitals

AbstractThe antimicrobial resistance or otherwise of Coagulase-negative Staphylococci (CoNS) isolates from medical students of some South Eastern Nigerian Universities were determined. Swab samples were obtained from the students and were cultured for CoNS using standard microbiological methods. Isolates obtained were assayed for antibiotic resistance using disc diffusion method. MecA and blaZ genes on 533bp and 510bp respectively, of the isolates were amplify using the Polymerase Chain React...

ENHANCEMENT OF METHANE PRODUCTION BY CODIGESTION OF KERATIN AND CELLULOSIC FEEDSTOCKS AT MESOPHILIC AND THERMOPHILIC CONDITIONS USING MICROBIAL CONSORTIA

ABSTRACTNigeria has energy crisis, hence, there is urgent need to diversify the country’s energy resources towards renewable alternatives for economic and social transformation. Biogas generated from the anaerobic digestion of local biomass resources, will increase national energy security as well as reduce dependence on fossil fuels. Optimization of methane production by codigestion of poultry feather and sawdust using microbial consortia were investigated. The results of the molecular ide...

ISOLATION AND CHARACTERIZATION OF MICROORGANISMS ASSOCIATED WITH BIOREMEDIATION OF HYDOCARBON POLLUTED SITES

Abstract: The negative impacts of hydrocarbon pollutants on our environments and economic resources cannot be overemphasized. The need to harness the bioremediation potentials of microorganisms associated with hydrocarbon pollution becomes very imperative in order to mitigate the environmental impacts of petroleum hydrocarbons in our environments. The molecular identification of the microorganisms isolated from four automobile workshops at Ugbene 2 Abakpa Nike, Enugu,...

PRODUCTION OF COMPOSITE YOGHURT FROM BLEND OF SOY MILK AND COW MILK USING MIXED LACTIC STARTER CULTURES ISOLATED FROM AQUARAPHA YOGHURT

ABSTRACTThe need to improve our vegetable protein consumption in order to reduce the incidence of protein deficiency diseases cannot be over-emphasized. This research was premised on evaluating the appropriate levels of substitution of cow milk with soya milk to produce composite yoghurt. Eight hundred (800) grams of soya bean seeds after sorting out foreign materials and washing were usedto produce 4.0 liters of soya milk (20% total solids). Cow milk (20% TS) was blended with soya milk in th...

COVID-19 and the Emergence of a New Way of Living

The COVID-19 pandemic is undoubtedly one of the biggest global crisis of this generation – a constantly evolving one, with new norms, regulations and updates to counter the disease. This article specifically talks on the introduction to COVID-19, its causes and effects and how we adapt to a new way of living. Keywords: Environment, Annual rate, Global crisis, Foreign investors, Epidemiology, Psychology.IntroductionThe outbreak of Coronavirus Disease (COVID-19) has been declared...

Diffusivity Of Chloride And Sulphate Ions Into Mortar Cubes Made Using Ordinary Portland And Portland Pozzolana Cements

ABSTRACTCement is subject to degradation by aggressive media found in the environment. Durability tests are therefore necessary for any cementitious material in a given aggressive environment. This study investigated chloride and sulphate diffusivity in mortar made from selected Kenyan cements. The test cements included three brands of Ordinary Portland Cements (OPC) and three brands of Portland Pozzolana Cements (PPC). The test cements were used to make mortar prisms at different water / cem...

Repellence Of Essential Oil Of Nigella Sativa L. Seeds Against Anopheles Gambiae And Identification Of The Active Blend

ABSTRACTAnopheles gambiae mosquitoes are vectors of malaria because of their ability to transmit Plasmodium falciparum parasites. The major impact of malaria is in sub-Saharan Africa where at least 90% of the deaths from malaria occur. In Kenya, malaria accounts for 30% of all outpatients and 19% of all admissions to health facilities. Malaria can also affect the quality of labour negatively and also can lead to low productivity through absenteeism. Anopheles gambiae is the vector associated ...

The Spectrum Of Bacteria Causing Urinary Tract Infection Among Pregnant Women Attending Sabasaba Clinic And Their Susceptibility To Antimicrobial Agents

ABSTRACT Urinary tract infections (UTI) during pregnancy can lead to serious obstetric complications, poor maternal and perinatal outcomes. The purpose of this study was to determinethe prevalence of bacterial UTI in pregnant women in Morogoro municipality, Tanzania. This study was conducted at SabaSaba Health Centre from May 2014 to July 2014 involving 196 pregnant women. Mid stream of urine samples were obtained and analyzed by dipstick and processed for culture. Out of 196 pregnant ...

Food Additives And Hygienic Status Of Flavoured Fruit Juices Manufactured In Dar Es Salaam, Tanzania

ABSTRACT The purpose of this study was to assess the quality of juices manufactured and sold for immediate consumption in Dar es Salaam Municipalities, Tanzania. The assessments were based on identifying the levels of preservatives, hygienic practices in manufacturing industries and determine microbiological quality of the juices. High Performance Liquid Chromatographic (HPLC) method was modified and validated for determination of benzoic and sorbic acids, while direct titration met...

Epidemiological Study Of Infectious Bursal Disease Virus In Selected Districts Of The Copperbelt Province In Zambia

ABSTRACT Infectious bursal disease (IBD) is a highly contagious disease of young chickens between 3 and 6 weeks of age. It is caused by infectious bursal disease virus(IBDV) which occursworldwide affecting livelihoods of resource - compromised poor communities. In Zambia, there is scantily documented information on the epidemiology of IBD. In-depth knowledge on the epidemiology of IBD is needed for effective control measures. This study aimed at molecular detection of circulating IBDV s...

Isolation, Identification And Antibiogram Of Salmonella Enterica From Chicken Eggs Collected From Selected Wards In Morogoro Municipality, Tanzania

ABSTRACTEggs provide protein to human. However, consumption of contaminated eggs with pathogenic Salmonellae (Salmonella enterica) imposes negative impacts to human health causing salmonellosis. The present study aimed at isolation, identification and antibiogram testing of Salmonella enterica from chicken eggs in Morogoro Municipality. A total of 570 raw, boiled, cracked, clean, and faecal soaked chicken eggs (120 local and 450 exotic) were collected from selected areas and examined from Nov...

Bacterial Contaminants Of African Indigenous Leafy Vegetables And Their Antibiotics Sensitivity Characteristics: A Case Study Of Morogoro Municipality, Tanzania

ABSTRACTA study was conducted in Morogoro Municipality, Tanzania to investigate the bacterial load found on African indigenous leafy vegetables, their sources and their sensitivity to commonly used antibiotics. A total of 126 samples of fresh African indigenous leafy vegetables (amaranth, nightshade and sweet potato leaves), water used for irrigation from rivers and shallow wells and manure fertilised soils were collected at farm sites and market outlets for bacteriological analysis. Bacteria...

Prevalence And Related Risk Factors Of Porcine Cysticercosis And African Swine Fever In Selected Urban/Peri-Urban Areas Of Morogoro, Tanzania

ABSTRACTThis study was carried out to estimate the prevalence and related risk factors of porcinecysticercosis (caused by Taenia solium) and African swine fever (ASF) in domestic pigs,and assesses the state of pork inspection in urban/peri-urban areas of Morogoro region,Tanzania, between November 2010 and January 2011. A two stage random sampling wasemployed. A total of 260 live pigs were tested serologically. Serum samples were testedfor the presence of circulating parasite antigen using a m...

Investigating The Perfomance Of Lateral Flow Devices In Diagnosis And Genetic Characterization Of Foot And Mouth Disease Virus In Tanzania

ABSTRACTA study on rapid detection and diagnosis of foot and mouth disease (FMD) field outbreaks was carried out in field settings using a Svanodip® foot and mouth disease virus (FMDV)- antigen (Ag) test lateral flow device (LFD) in Serengeti and Ngorongoro districts of Tanzania. Epithelial tissues from cattle (n=80) were collected from different villages and tested at penside using Svanodip® FMDV-antigen test to evaluate the sensitivity and specificity of LFDs relative to gold standard rev...


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