Research Papers/Topics in Applied Microbiology

Review on Phosphate Solubilizing Fungi

Phosphorus (P) is the second important nutrient after nitrogen. In plants, phosphorous increases the strength of cereal straw, promotes flower formation and fruit production, stimulates root development and essential for seed formation. Mobility of phosphate ions in the soil is very low due to their high retention in soil. In this regard, P supply through biological systems is considered a viable alternative, and inoculation of Phosphate solubilizing microorganisms, especially fungi to soil, ...

Review on Microbial Production of Organic Acid

The first individual process for citric acid production was the liquid surface culture (LSC), which was introduced in 1919 by Société des Produits Organiques in Belgium, and in 1923 by Chas Pfizer & Co. in US. After that, other methods of fermentation, such as submerged fermentation were developed. Although this technique is more sophisticated, surface method required less effort in operation and installation and energy cost. Organic acids such as citric, malic and pyruvic are used e...

Review on Biodegradation of Organic Compounds and Plastics

Biodegradation is defined as the biologically catalyzed reduction in complexity of chemical compounds. Indeed, biodegradation is the process by which organic substances are broken down into smaller compounds by living microbial organisms. When biodegradation is complete, the process is called "mineralization". However, in most cases the term biodegradation is generally used to describe almost any biologically mediated change in a substrate. So, understanding the process of biodegradati...

BACTERIAL CONTAMINATION OF UGANDAN PAPER CURRENCY NOTES POSSESSED BY FOOD VENDORS AROUND MULAGO HOSPITAL COMPLEX

BACTERIAL CONTAMINATION OF UGANDAN PAPER CURRENCY NOTES POSSESSED BY FOOD VENDORS AROUND MULAGO HOSPITAL COMPLEX Muhumuza Allan1, Catherine Atuhaire2, Musisi Nathan3, Francis Ejobi4, Samuel Nambile Cumber5*1School of Biosecurity, biotechnical and Laboratory Sciences, College of Veterinary Medicine, Animal Production and Biosecurity, Makerere University, Uganda2Faculty of medicine, Department of Nursing, Mbarara University of Science and Technology, Uganda 3School of Biosecuri...

Co-infection of HIV and Some Opportunistic Pathogens Among Patients Attending Two Referral Hospitals in Akure

ABSTRACTThe  seroprevalence  studies  of  Plasmodium  falciparum,  Mycobacterium  tuberculosis  and Samonella typhi, HIV and co-infections by the four pathogens were carried out among patients attending Don Bosco Medical centre Akure and Ondo State Specialist Hospital, Akure. Venous blood were collected from a total of 500 randomly selected patients aged 1 to ≥ 91 years  that attended the hospitals  for routine medical check up. Diagnostic test kits (“Determine” and ‟Unigold...

Excerpt of fungi culti

Mushroom is a macro fungus with a distinctive fruiting body which can be either epigeous or hypogeous and large enough to be seen with the naked eye and can be picked with hand (Chang and Milles.19920).           Mushrooms derive all of their energy and growth materials from their growth medium, through biochemical decomposition processes (Chang, Shu-Ting, et al. 2004).

Antimicrobial Activity, Chemical Compositions and Proximate Analysis of Ixora Coccinea L. Leaves on Some Clinical Pathogens

Plants derived bioactive compounds have been the centre of recent owing to their health promoting effects. An attempt was made in evaluating the phytochemical, minerals, proximate and anti-microbial activity of analyses standard agar well diffusion method saponins, tannins, flavon minerals analysis revealed the presence of manganese and phosphorus. Highest mineral content was recorded in magnesium (21. Proximate analysis showed the presence of ash (10.3%), moisture (7.10%), protein (16.45%),...

Comparative Study Between the Eect of Parkia Biglobosa (JACQ) benth and Conventional Antibiotics Against Multiple Antibiotic Resistant Uropathogenic Bacteria (MARUB)

The purpose of this work is to compare the effect of convectional antibiotics with medicinal plant (Parkia biglobosa (Jacq.)Benth) against multiple antibiotics resistant Uropathogenic bacteria isolates.(MARUB), which was isolated from basically two hospital in Ondo State, Nigeria. The two hospitals are Gani Fawehinmi Diagnostic center, Ondo and Federal Medical Center, Owo between July, 2017 and October, 2017. fifty two (52) Gram negative bacteria isolated from urine of UTIs patient at the Ga...

Antibacterial Activity of Garlic (ALLIUM SATIVUM) on Staphylococcus aureus and Escherichia coli

The study was carried out to evaluate the antibacterial potential of ethanolic and aqueous extracts of Garlic on Staphylococcus aureus and Escherichia coli by serial dilution in molten agar. The result of the study showed that both aqueous and ethanolic extracts inhibited the growth of the test organisms and hence their potential for the development of novel classes of antibiotics. 

In Vitro Antibacterial Activity and Toxicity Study of Eucalyptus Camaldulensis Leaves Extracts against Clinical Isolates of Salmonella Sp

ABSTRACTEucalyptus camaldulensis is an important medicinal herb in traditional healthcare practice and has been reported for various biological activities. The objective of the present study was to analyze the antibacterial potentials and toxicity study of E. camaldulensis leave extracts against clinical isolates of salmonella species. The E. camaldulensis leaves were extracted separately and successively with ethanol, water and methanol using percolation method. The extracts were tested in v...

Purification and Characterization of Lipase from Aspergillus flavus PW2961 using Magnetic Nanoparticles

________________________________(Received: 08:03:2017; Accepted: 05:04:2017) _______________________AbstractLipase from Aspergillus flavus was purified in a single step purification using MnFeO4 magnetic nano particles to achieve a 20.53- fold purification with specific activity of 11.29 U/mg and a 59% recovery yield. SDS-PAGE of lipase showed a single pure band with corresponding molecular weight of 35 kDa. The optimal temperature and pH for the enzyme activity were 45°C and 7.0 respect...

CORN FERMENTATION AND ITS IMPORTANCE IN BIOFUEL PRODUCTION

ABSTRACT Bio-fuels are prominent in current discussions both as a solution to problems and as a creator of problems. They have promise as a substitute for fossil fuels, particularly petroleum as the raw material for transportation fuel. But bio-fuels also have pitfalls, especially when produced at a scale sufficient to replace a significant proportion of the world's use of petroleum. This controversial topic has attracted great political, economic and social attention because of...

ANTIBIOTIC SENSITIVITY TESTING ON EGGS FROM LAYERS TREATED WITH ANTIBIOTIC

ANTIBIOTIC SENSITIVITY TESTING ON EGGS FROM LAYERS TREATED WITH ANTIBIOTIC   A RESEARCH PROJECT   ABSTRACT Antibiotic sensitivity testing is the susceptibility of bacteria to antibiotics, it is carried out to determine which antibiotic will be most successful in treating a bacterial infections. The total mean colony count of the bacterial isolates in the two set of egg sample  carried out showed the mean total bacterial count as 0.33x102 – 3.6 x 102 ...

ANTIBIOGRAM OF BACTERIAL ISOLATED FROM SEALED AND UNSEALED DRUGS

ANTIBIOGRAM OF BACTERIAL ISOLATED FROM SEALED AND UNSEALED DRUGS   A RESEARCH PROJECT ABSTRACT The study on the isolation and antibiogram of bacterial isolated form sealed and unsealed drugs revealed total Bacteria count ranging form 1.2 X 107 to 11.5 X108cfu/g for unsealed and 1.5 X107 to 9.2 x 107cfu/g  for sealed. The following bacterial isolates were identified these include Bacillus sp, klebsiellssp, staphylococcus sp Escherichia sp, Lactrbacillussp, proteussp an...

Evaluation of Inhibitory Zone Diameter (IZD), Phytochemical Screening, Elemental Composition and Proximate Analysis of Crude Cleistopholis Patens Leaf

This purpose of this study is to determine the Inhibitory zone diameter (Izd), phytochemical screening, elemental composition and proximate analysis of crude Cleistopholis patens leaf, bark, aqueous and ethanol extracts on selected pathogenic isolates. The leaf and bark of Cleistopholis patens plants were obtained from a location in the southwestern part of Nigeria, in the tropical rainforest of Ikare Akoko, Ondo state and Ile Ife, Osun state, Nigeria. The six (6) bacterial isolates include E...


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