Research Papers/Topics in Applied Microbiology

Codigestion of Oryza sativa Husks and Musa paradisiaca Peels for the Production of Biogas

Oryza sativa husks and peels of Musa paradisiaca were pretreated by hydrothermal and acid fermentation using cassava mill effluent, and co-digested in a laboratory scale reactor maintained at 45 ± 2 ⁰C for 42 days. Five digesters were used for the experiment and each digester contained equal proportion of Oryza sativa husks and Musa paradisiaca in the ratio of 1:1; and cow dung (the inoculum) was added across. Substrate in Digester X (Control) was not pretreated. Digester A contained Oryza...

Comparative adsorption of amylase, protease and lipase on ZnFe2O4: kinetics, isothermal and thermodynamics studies

Abstract The role of enzyme engineering in biotechnol-ogy, biological and pharmaceutical process cannot be overemphasized. This study compared the adsorption ofdigestives enzymes; amylase, protease and lipase on to Zn-ferrite (ZnFe2O4). The metal ferrite was synthesized via asol–gel technique and characterized with scanning electronmicroscopy (SEM), X-ray diffraction (XRD), Electronparamagnetic resonance (EPR) and Fourier transforminfrared spectroscopy (FTIR). The adsorption was studiedin a...

Application of mycology in forensic science

Abstract Forensic Mycology is the application of Mycology for producing evidence in terms of criminal investigation and their further evaluation in the court of law. The problem with relative neglect of Mycology in Forensic science is currently leading to insufficient knowledge, in order to support the needs for fungal expertise in areas of criminal investigation. Mucor hiemalis and Penicillium griseofulvum had been used to determine the minimum interval since death, Coprinus comatu...

MICROBIOLOGICAL EFFECT OF FERMENTATION ON THE NUTRIENT, ANTINUTRIENTS AND FUNCTIONAL PROPERTIES OF FERMENTED LIMA BEANS SEEDS (Phaseolus lunatus).

ABSTRACT Semi- solid raw Lima beans (Phaseolus lunatus) were fermented for a period of ten days, chemical (proximate composition, antinutritional contents, pH, and total titratable acidity) and physical changes (temperature, odour, and texture) occurring during the fermentation of the lima beans were monitored. The fermentation was carried out for 48, 96, 120, 168 and 192 hours respectively at ambient temperature. The microbial load was as well monitored. Microorganisms isolated fr...

EFFECTS OF FERMENTED PALM WINE ON SOME DIARRHOEAGENIC BACTERIA

The effect of fermented palm wine tapped from Raphia palm tree (Raphia hookeri) on the growth ofsome common diarrhoeagenic bacteria such as Staphylococcus aureus, Escherichia coli,Salmonella typhi, Shigella dysentariae and Esherichia coli 0157:H7 was carried out using agardiffusion method. The palm wine used had growth inhibitory effect on all the test organisms withdiameter of zones of inhibition ranging from 2.20mm to 28.20mm. Palm wine subjected tofermentation for 168hours (7 days) exerted...

Improvement of the Physicochemical and Microbiological Quality of the Date’s Fruit Product in South Algeria by Adding Rosemary Herb

The dates are used to prepare several food products, among which the Btana is a best conservation form commonly practiced in the South-west area of Algeria. The peasants add during its preparation one or more aromatic herbs according to the choice and the areas. The present research aims relates to the study of the effectiveness of the methods used from the microbiological quality point of view and the determination of the effect of the plants added during the Btana preparations, for that...

Generation of bioethanol from common date by-products, “Teggaza and Lebghel” in Southern Algeria

Date by-products constitute the principal food for the oasis populations in Middle East and North Africa. Dates contents consist of 70 to 80% of reducing sugars, and do not require an intensive energy and labour for thermophysical pre-treatment. They can serve as a good feedstock for bioethanol generation through fermentation and distillation. Algeria is among the top sixth producers of dates in the world with more than 250,000 tons/year; from these, more than 30% can be lost for dif...

Prebiotics-a viable prophylaxis against Salmonella typhii

SummarySo far, majority of the preventive effect of prebiotics has been on animal models. However, several in vitro studies using 2D and 3D cell cultures mimicking the environmental condition as well as various organisms present within the gut of man had shown the efficacy of prebiotics in reducing the attachment of enteropathogenic organisms like S. typhi to the gut epithelium. In vivo studies with human have been able to prove its efficacy in preventing diseases such as Inflammatory b...

Evaluation of the phytochemical composition and antimicrobial activity of lemongrass (Cymbopogon citratus) against some human pathogenic microorganisms

ABSTRACTAntibiotic resistance has become a wide spread problem. The efficacy of two solvent extracts of Cymbopogon citratus against some test bacteria and fungi was evaluated. The test bacteria was; Staphylococcus aureus, and the test fungi was; Candida albicans. The phytochemical properties and the antimicrobial potency of Cymbopogon citratus (lemon grass) against selected microbial pathogens using chloroform and methanol as extracting solvents were assessed. The minimum inhibitory, bacteric...

Hepatitis B infection

INTRODUCTION        Hepatitis B is a viral disease process caused by the Hepatitis B virus (HBV). Hepatitis B virus is a major cause of acute and chronic hepatitis worldwide and is more prevalence in developing countries. More than 2 billion people are infected with HBV worldwide while some 280million are chronic carriers, harbouring the virus in their liver. The disease is responsible for 80% of all cases of primary liver cases, which is one of leadin...

An investigation on the activities of amylase, cellulase and pectinase from Bacillus and Aspergillus species isolated from stale fast food sample

Abstract:An investigation on the activities of amylase, cellulase and pectinase from Bacillus and Aspergillus species isolated from stale fast food samples was carried out. The freshly prepared samples were of Mince pie, Scotch egg, Doughnuts, Sausages and Fishrolls obtained from fast food centres in Ikeja, Lagos, Nigeria. The Bacillus isolates were identified as Bacillus subtills, Bacillus pumilus, Bacilus licheniformis, Bacillus polymyxa and Bacillus brevis. All the isolated bacilli stra...

Bacteriological analysis of flooded soil in Benin areas, Edo State

ABSTRACT Microorganisms are important components in the structure and function of organic matter in soil, hence this work was done to identify bacterial communities present in flooded soil. Soil samples were collected from eight different areas within Benin metropolis, Edo State, Nigeria. The samples were cultured on using pour plate method, identification of the main bacterial groups was carried out based on cultural, morphology and biochemical test. The results obtained showed the to...

Socioeconomic determinants of infants mortality rate in Zaria LGA of Kaduna Nigeria

Socioeconomic determinants of infants mortality rate in Zaria LGA of KadunaABSTRACT Infant mortality remains high in Zaria Local Government and beyond despite the various policies that have been implemented by the authorities to reduce it. Although studies have examined various factors that explain infant mortality, effects of infant mortality and causes of infant mortality has been rarely explored. The study finds out socio-demographic strength of the respondents, causes of infant mo...

Review on Role of Phosphate Solubilizing Microorganisms in Sustainable Agriculture

Most of soil microorganisms are known for their ability to solubilize insoluble phosphorous compounds for releasing soluble phosphate to make phosphorous available for plant absorption. Releasement of this soluble phosphorous is very important in increasing plant growth and plant product yields. Inoculating plant seeds with phosphate solubilizing microorganisms is very effective and environmentally useful to decrease the side effect of chemical fertilizers on soil microbes and other org...

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