Applied Microbiology Research Papers/Topics

Exposure to Salinity and Light Spectra Regulates Glucosinolates, Phenolics, and Antioxidant Capacity of Brassica carinata L. Microgreens

Abstract: The effect of salt treatment on Brassica carinata (BC) microgreens grown under different light wavelengths on glucosinolates (GLs) and phenolic compounds were evaluated. Quantifiable GLs were identified using ultra-high performance-quadrupole time of flight mass spectrometry. Extracts’ ability to activate antioxidant enzymes (superoxide dismutase (SOD) and catalase (CAT)) was evaluated on human colorectal carcinoma cells (HCT116). Furthermore, BC compounds’ ability to activate ...

Multi-source spatial data-based invasion risk modeling of striga (Striga asiatica) in Zimbabwe.

Abstract: Monitoring of destructive invasive weeds such as those from the genus Striga requires accurate, near real-time predictions and integrated assessment techniques to enable better surveillance and consistent assessment initiatives. Thus, in this study, we predicted the potential ecological niche of Striga (Striga asiatica) weed in Zimbabwe, to identify and understand its propagation and map potentially vulnerable cropping areas. Vegetation phenology from remote sensing, bioclimatic an...

Detection of Extended Spectrum Beta-Lactamase Production in Escherichia coli Isolated from Cattle Faeces in Owo Metropolis

Escherichia coli which synthesize extended spectrum beta-lactamases (ESBL) have been implicated in severe human diseases. There is substantial evidence that cattle faeces have a role in developing and spread of multidrug-resistant pathogens, raising public health concerns. The study is aimed at detecting ESBL-producing E. coli associated with cattle faeces within Owo metropolis. Freshly passed faecal samples were taken aseptically from 9 apparently healthy cattle that were about to be sl...

Studies On Microbiological And Physico- Chemical Analyses Of Commercially And Laboratory Prepared Orange Juices

ABSTRACT Microbiological and physico-chemical analyses and sensory evaluation were carried out on commercially and freshly prepared orange juice (100%) in the laboratory and its shelf- life after the storage period of 90 days using various storage methods. At the end of the study, the result showed that the laboratory processed orange juice in terms of the nutritional composition when compared with the commercially processed orange juice had a better quality considering the parameters assess...

Microfluidics for Drug Screening and Paralysis of Parasites

The emergence of new drugs is often driven by the escalating resistance of parasites to existing drugs and the accessibility of more advanced technology platforms. Microfluidic devices have allowed for quicker testing of compounds, regulated sampling/sorting of entire animals, and automated behavioral pattern detection. In the majority of microfluidic devices, the effects of drugs on small animals (e.g. Caenorhabditis elegans)elegant are quantified by an endpoint, dose response curve that sho...

Microfluidic chip for analyzing the effects of drugs and their dosage on the locomotion behavior of parasitic worms

This new microfluidic platform offers a more sensitive method for screening drugs and their effects on the locomotion of free living and parasitic nematodes. The chip is capable of monitoring four nematode parameters (number of worms responsive, number of worms leaving the drug well, average worm velocity and time until unresponsiveness) to provide a more detailed analysis and resolution of drug resistance. The drug screening experiment is divided into three parts: pre-exposure study, dose ex...

Droplet Actuation Platform for Chemical Sensors

An open microfluidic system has been developed to dispense and manipulate individual droplets on plastic sheets. Superhydrophobic material is applied to the surface of obtainable plastic sheets by spraying, then hydrophilic symbols are printed with an inkjet printer. The plastic sheets are connected to a two-axis tilting platform driven by stepper motors, which produces mechanical agitation enabling droplet transport. The system has been demonstrated to carry out various droplet operations, s...

Isolation and Identification of Bacteria in Broiler Chicken Meat Around Dar es Salaam Region

ABSTRACT Broiler chicken meat is now considered as a major fast-growing source of meat in many parts of Tanzania especially Dar es salaam. The consumers demand safe and hygienic products without contamination with pathogenic microorganisms when the production and consumption of broiler chicken meat is gradually increasing. The present study was conducted to assess the bacterial contamination of broiler chicken meat collected from different areas around Dar es salaam especially Temeke and...

Extraction of DNA in Tomatoes and Garden Eggs Fruits

ABSTRACT This project is basically concerned with the extraction of DNA in tomatoes and garden egg fruit. An experiment on how to extract DNA on tomatoes and garden egg, isolation on it to see what DNA actually look like as well as importance of DNA extraction are consid3ered in this project. TABLE OF CONTENT Title page ……………………………………………………… i. Certification……………………………………………………..ii Dedication………………...

Antimicrobial Resistance Pattern of Coagulase-negative Staphylococci isolates from Medical Students of Some Nigerian Teaching Hospitals

Abstract The antimicrobial resistance or otherwise of Coagulase-negative Staphylococci (CoNS) isolates from medical students of some South Eastern Nigerian Universities were determined. Swab samples were obtained from the students and were cultured for CoNS using standard microbiological methods. Isolates obtained were assayed for antibiotic resistance using disc diffusion method. MecA and blaZ genes on 533bp and 510bp respectively, of the isolates were amplify using the Polymerase Chain Reac...

ENHANCEMENT OF METHANE PRODUCTION BY CODIGESTION OF KERATIN AND CELLULOSIC FEEDSTOCKS AT MESOPHILIC AND THERMOPHILIC CONDITIONS USING MICROBIAL CONSORTIA

ABSTRACTNigeria has energy crisis, hence, there is urgent need to diversify the country’s energy resources towards renewable alternatives for economic and social transformation. Biogas generated from the anaerobic digestion of local biomass resources, will increase national energy security as well as reduce dependence on fossil fuels. Optimization of methane production by codigestion of poultry feather and sawdust using microbial consortia were investigated. The results of the molecular ide...

ISOLATION AND CHARACTERIZATION OF MICROORGANISMS ASSOCIATED WITH BIOREMEDIATION OF HYDOCARBON POLLUTED SITES

Abstract:  The negative impacts of hydrocarbon pollutants on our environments and economic resources cannot be overemphasized. The need to harness the bioremediation potentials of microorganisms associated with hydrocarbon pollution becomes very imperative in order to mitigate the environmental impacts of petroleum hydrocarbons in our environments. The molecular identification of the microorganisms isolated from four automobile workshops at Ugbene 2 Abakpa Nike, Enugu, Nigeria based on 1...

PRODUCTION OF COMPOSITE YOGHURT FROM BLEND OF SOY MILK AND COW MILK USING MIXED LACTIC STARTER CULTURES ISOLATED FROM AQUARAPHA YOGHURT

ABSTRACTThe need to improve our vegetable protein consumption in order to reduce the incidence of protein deficiency diseases cannot be over-emphasized. This research was premised on evaluating the appropriate levels of substitution of cow milk with soya milk to produce composite yoghurt. Eight hundred (800) grams of soya bean seeds after sorting out foreign materials and washing were usedto produce 4.0 liters of soya milk (20% total solids). Cow milk (20% TS) was blended with soya milk in th...

COVID-19 and the Emergence of a New Way of Living

The COVID-19 pandemic is undoubtedly one of the biggest global crisis of this generation – a constantly evolving one, with new norms, regulations and updates to counter the disease. This article specifically talks on the introduction to COVID-19, its causes and effects and how we adapt to a new way of living. Keywords: Environment, Annual rate, Global crisis, Foreign investors, Epidemiology, Psychology. Introduction The outbreak of Coronavirus Disease (COVID-19) has been declared a Publi...

Diffusivity Of Chloride And Sulphate Ions Into Mortar Cubes Made Using Ordinary Portland And Portland Pozzolana Cements

ABSTRACT Cement is subject to degradation by aggressive media found in the environment. Durability tests are therefore necessary for any cementitious material in a given aggressive environment. This study investigated chloride and sulphate diffusivity in mortar made from selected Kenyan cements. The test cements included three brands of Ordinary Portland Cements (OPC) and three brands of Portland Pozzolana Cements (PPC). The test cements were used to make mortar prisms at different water / ce...


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