Antibiotics Susceptibility Pattern of Different Bacteria Associated With Wound Sepsis (A Case Study of University of Ilorin Teaching Hospital)

TABLE OF CONTENT

Tile Page                                                                                                         i

Approval Page                                                                                                ii

Dedication                                                                                                      iii

Acknowledgment                                                                                           iv

Table of Content                                                                                             v

List of Figures

List of Table                                                                                      

Abstract

CHAPTER ONE

1.0       Introduction                                                                                        1

1.1       Problem of Statement                                                                         13                              

1.2       Justification                                                                                       14   

1.3       Aim and Objective of the study                                                         15

CHAPTER TWO

2.0       Literature Review                                                                               17       

CHAPTER THREE

3.0       Materials and Method                                                                        29

3.1       Sterilization of Materials                                                                    29

3.2       Sample Size                                                                                        29

3.3       Collection and Processing Sample                                                     30

3.4       Culture Media                                                                                     30

3.5       Isolation of Cultures                                                                           31

3.6       Characterization of Bacteria Isolates                                                 32

3.7       Colonial Morphology                                                                         32

3.8       Cellular Morphology                                                                          32

3.9       Biochemical Characterization                                                            34

3.10     Antibiotics Sensitivity Test                                                                36

CHAPTER FOUR

4.0       Results                                                                                                39

CHAPTER FIVE

5.0       Discussion                                                                                          60

5.1       Conclusion                                                                                          66

5.2       Recommendation                                                                               67

References

 

LIST OF FIGURES

Figure 1:         Frequency of Occurrence of bacterial from Wound Infection patient in University of Ilorin Teaching Hospital.

Figure 2:         Bacterial Isolate from patient with diabetic ulcers in University of Ilorin Teaching Hospital.

Figure 3:         Bacterial Isolated from patients with surgical wound in University of Ilorin Teaching Hospital.

Figure 4:         Bacterial Isolated from patients with burns in University of Ilorin Teaching Hospital.

Figure 5:         bacterial Isolated from patient with pressure sore in University of Ilorin Teaching Hospital.

LIST OF TABLE

Table 1:           Morphological Characteristics, Staining Reaction and Biochemical Test

Table 2:           Frequency of Occurrence of bacteria from Wound Infection patient in University of Ilorin Teaching Hospital.

Table 3:           Bacteria pathogens causing wound infection in patients with Diabetic ulcers.

Table 4:           Bacteria pathogens causing wound infection in patients with Surgical Wound.

Table 5:           Bacteria pathogens causing wound infection in patients with Burns.

Table 6:           Bacteria pathogens causing wound infection in patients with Pressure Sore.

Table 7:           Antibiotic susceptibility Patterns of Staphylococcus aureus isolated from wound patients in UITH

Table 8:           Antibiotics susceptibility pattern of Pseudomonas aeruginosa isolated from wound in UITH

Table 9:           Antibiotic susceptibility pattern of Staphylococcus epidermidis in UITH.

Table 10:         Antibiotic Susceptibility pattern of Escherichia coli isolated from wound patients in UITH.

Table 11:         Antibiotic Susceptibility pattern of Streptococcus pyogenes isolated from wound patients in UITH.

Table 12:         Antibiotic Susceptibility pattern of Klebsiella pneumoniae isolated from wound patients in UITH.                                          

 

 

                                                 

 

 

 

 

 

 

 

ABSTRACT

This work investigated the antibiotic susceptibility profile of bacterial associated with wound sepsis of patients attending University of Ilorin, Ilorin, Teaching Hospital. Wound swabs were collected from a total number of Hundred patients with different kinds of wound (surgical wound, burn, ulcers and pressure sores) and cultured, of which 72 samples showed bacterial growth. Six different species of bacteria were isolated. Staphylocococcus aureus (47.2%) Pseudomonas aeruginosa (19.5%), Klebsiella pneumonia (6.85%), Escherichia coli (15.3%), Staphylococcus epidemidis (5.6%) and Streptococcus pyogenes (5.6%). The antibiotics susceptibility test of these bacterial isolate was performed using the Kirby- bauer dist diffusion method. Gantamycin and Sreptomycin shows high effectiveness to all the isolate except Staphylococcus epidermis and Klebsiella with pefloxacin and ceftadine respectively showing sensitivity to them. Resistance were shown amongst Ampicillin, contrimoxazole and Tetracycline. This study has revealed Gentamycin as the only antibiotic to which most bacterial isolate from infected wounds were sensitive to. Ampicillin and penicillin were effective against Streptococcus pygenes while zinacef and ceftazidime shows high effectiveness against Klebsiella pneumonia.

KEYWORDS: wound infection: antibiotics susceptibility profile: staphylococcus aureus: gentamycin.