ANTIMICROBIAL ACTIVITY AND PHYTOCHEMICAL COMPOSITION OF CRUDE ETHYL ACETATE EXTRACTS OF ANISOPUS MANNI

 Anisopus mannii(Asclepiadaceae) –is a glabrous twining shrub, strong climber with greenish flower in globose, lateral umbelliform cymes it is widely used as an anti-diabetic agent in Northern and southern Nigerian traditional medicine, Studies were conducted to determine the phytochemical constituents of leaf and bark ethyl acetate extracts of Anisopus mannii and to evaluate its antimicrobial activity, using the agar well diffusion technique using standard phytochemical analysis procedures, results revealed the presence of saponins, alkaloids, flavonoids, glycosides, and tannins. steroids and anthraquinones were not detected in the extracts. Antimicrobial activities were assayed using the concentrations of 60mg/ml, 30mg/ml, 15mg/ml and 7.5mg/ml. the Extracts dose dependently inhibited the growth of Escherichia coli, Staphylococcus aureus, Salmonella typhi, Klebsiellapneumoniae, and pseudomonas aeruginosa. The minimal inhibitory concentration (M.I.C) values were 15 mg/ml for Escherichia coli, 30mg/ml for Staphylococcus aureus, Salmonella typhi, and 60mg/ml for Klebsiellapneumoniae and Pseudomonas aeruginosa. The proximate analysis showed that the plant contained low fat (6.50%, 6.18%), high carbohydrate content (42.00%, 40.23%) in leaves and bark extracts respectively.These results suggest thatthe leaf and bark extracts of Anisopus mannii could be promising in its potential usefulness for treatment of bacterial induced ailments and a good dietary source of nutrients.The purpose of this work is to determine the antimicrobial activities on selected clinical organisms and element composition, Anti-nutrients, Proximate contents of Anisopus mannii