ABSTRACT
Plant samples collected within and around Federal University Oye-Ekiti was air dried, pulverized and subjected to hot (soxhlet) extraction and cold extraction using three (3) extractive solvent (ethyl acetate, methanol, n-hexane).' The extracts were seperated from the solvent using rotary evaporator while the crude extracts were subjected to thin layer chromatography (TLC) using different solvent ratio mixtures on a silica gel coated TLC plates. The antibacterial activity of active constituents of each crude extracts were determined using Bioautography (agar-overlay) method by spraying the TLC plates with tetrazoliurn saJt and incubated for one hour. Zones of inhibition were observed for each extract on the chromatogram. The result revealed varying retention factor {R/) of different extract ranging from 0.05-0.72 while the total number of active bands range between 1 to 6. the zones of growth inhibition recorded among the three different solvent of extraction on cold and hot extraction range between 14rnm-34rnm. This results show that there are bioactive constituent present in Diodia scandens and Bioautography (agar-overlay) method can be used in detecting them.
, A & OLALEKAN, C (2021). Detection Of Bioactive Compounds By Bioautography Of Diodia Scandens On Staphylococcus Aureus And Coagulase Negative Staphylococcus. Afribary. Retrieved from https://afribary.com/works/detection-of-bioactive-compounds-by-bioautography-of-diodia-scandens-on-staphylococcus-aureus-and-coagulase-negative-staphylococcus
, ATITEBI and CHIEDU OLALEKAN "Detection Of Bioactive Compounds By Bioautography Of Diodia Scandens On Staphylococcus Aureus And Coagulase Negative Staphylococcus" Afribary. Afribary, 20 May. 2021, https://afribary.com/works/detection-of-bioactive-compounds-by-bioautography-of-diodia-scandens-on-staphylococcus-aureus-and-coagulase-negative-staphylococcus. Accessed 05 May. 2024.
, ATITEBI, CHIEDU OLALEKAN . "Detection Of Bioactive Compounds By Bioautography Of Diodia Scandens On Staphylococcus Aureus And Coagulase Negative Staphylococcus". Afribary, Afribary, 20 May. 2021. Web. 05 May. 2024. < https://afribary.com/works/detection-of-bioactive-compounds-by-bioautography-of-diodia-scandens-on-staphylococcus-aureus-and-coagulase-negative-staphylococcus >.
, ATITEBI and OLALEKAN, CHIEDU . "Detection Of Bioactive Compounds By Bioautography Of Diodia Scandens On Staphylococcus Aureus And Coagulase Negative Staphylococcus" Afribary (2021). Accessed May 05, 2024. https://afribary.com/works/detection-of-bioactive-compounds-by-bioautography-of-diodia-scandens-on-staphylococcus-aureus-and-coagulase-negative-staphylococcus