ABSTRACT
Medicinal plants have been used for centuries as remedies for human diseases because they contain components of therapeutic value. Phyllanthus amarus has shown to work as an antifungal, antibacterial and antiviral agent. Bioautography is a technique used to isolate compounds by employing a suitable chromatographic process followed by a biological detection system. Referenced pure culture antibiotics resistant Coagulase Negative Staphylococcus strain (Co NS I Ob: Ojo) and Staphylococcus aureus (W24 I: Ojo) were obtained from the Drug Discovery and Development Research (DDDR) unit, Department of Microbiology, Federal University Oye Ekiti, confirmation tests were carried out on these organisms which showed that the two strains were Gram positive, cocci and ferment various sugars with production of acid. The extract of Phyllanthus amarus was gotten through both cold and hot (soxhlet) extraction, Thin Layer Chromatography-Bioautographic method was carried out on the extracts to detect the antibacterial activity of various bioactive constituent in Phyllanthus amarus against Staphylococcus strains. The result revealed various retention factors of the extracts in various solvent systems ranging from 0.096-0.838, with the total number of active bands ranging from 1-7. Different zones of inhibition were also recorded, ranging from l 8mm-27mm. The result shows that there are bioactive constituents present in Phyllanthus amarus and Bioautography (agar-overlay) can be used to detect them.
, O & VICTORIA, O (2021). Detection Of Bioactive Constituents In Phyllanthus AmarUs Using Thin Layer Chromatography-Bioautographic Method Against Staphylococcus Aureus And Coagulase Negative Staphylococcus AUreUs. Afribary. Retrieved from https://afribary.com/works/detection-of-bioactive-constituents-in-phyllanthus-amarus-using-thin-layer-chromatography-bioautographic-method-against-staphylococcus-aureus-and-coagulase-negative-staphylococcus-aureus
, ORIRE and OMOLOLA VICTORIA "Detection Of Bioactive Constituents In Phyllanthus AmarUs Using Thin Layer Chromatography-Bioautographic Method Against Staphylococcus Aureus And Coagulase Negative Staphylococcus AUreUs" Afribary. Afribary, 20 May. 2021, https://afribary.com/works/detection-of-bioactive-constituents-in-phyllanthus-amarus-using-thin-layer-chromatography-bioautographic-method-against-staphylococcus-aureus-and-coagulase-negative-staphylococcus-aureus. Accessed 22 Nov. 2024.
, ORIRE, OMOLOLA VICTORIA . "Detection Of Bioactive Constituents In Phyllanthus AmarUs Using Thin Layer Chromatography-Bioautographic Method Against Staphylococcus Aureus And Coagulase Negative Staphylococcus AUreUs". Afribary, Afribary, 20 May. 2021. Web. 22 Nov. 2024. < https://afribary.com/works/detection-of-bioactive-constituents-in-phyllanthus-amarus-using-thin-layer-chromatography-bioautographic-method-against-staphylococcus-aureus-and-coagulase-negative-staphylococcus-aureus >.
, ORIRE and VICTORIA, OMOLOLA . "Detection Of Bioactive Constituents In Phyllanthus AmarUs Using Thin Layer Chromatography-Bioautographic Method Against Staphylococcus Aureus And Coagulase Negative Staphylococcus AUreUs" Afribary (2021). Accessed November 22, 2024. https://afribary.com/works/detection-of-bioactive-constituents-in-phyllanthus-amarus-using-thin-layer-chromatography-bioautographic-method-against-staphylococcus-aureus-and-coagulase-negative-staphylococcus-aureus