ABSTRACT
Malaria is an enormous public health problem worldwide and kills one to two million people every year, mostly children residing in Africa . And the aim of this study is to determine the impact of combined treatment with Artesunate and Chloroquine on the white blood cell count of plasmodium berghei infected albino mice. And to compare and observe effect of combined treatment of chloroquine and artesunate on white blood cell count of P. berghei infected albino mice.
A total of 15 adult albino mice weighing between 21 to 25g were obtained from the Animal House in Delta State University Abraka, Delta State. The animals were randomly divided into three groups: I, II, and III. Group I, II, and III, were inoculated with the malaria parasites. Group I served as negative control and it was treated with 5% alcohol regularly for 5 days and Group II received daily doses of 4mg/kg for day 1 and 2mg/kg for day 2 to day 5 of Artesunate Combination Therapy (ACT), while Group III served as positive control and it received chloroquine syrup (10mg/kg for day 1 and day 2 and 5mg/kg for day 3) once daily for 3 days .
The result of this study show that group I mice which received 5% alcohol daily for 5 days had the highest mean of white blood cell count (14800.00± 1760.00), this could be due to the fact that 5% alcohol had no effect on the malaria parasite. The only logical conclusion from this observation is that plasmodium berghei parasite thrived in these mice to cause a more extensive destruction of white blood cells in them than for the other groups.
Group II and III mice had lower mean of white blood cell volume ( 4340.00 ± 273.00 and 4300.00± 3600.00) than that of group I. It is observed that the mean white blood cell volume of group II that had P. berghei infected mice treated with combined artesunate and chloroquine and group III treated with only chloroquine had no significant difference ( p < 0.05.)
In conclusion, the treatment with artesunate and chloroquine combination therapy on plasmodium berghei infected albino mice has a significant difference on white blood cell count.
TABLE OF CONTENT
Certification ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ____ _ i
Dedication ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ____ __ii
Acknowledgment ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ __iii
Table of content ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ___iv
Abstract ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ____ _____v
CHAPTER ONE
Introduction ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ____ ___
Objective of study___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ____ ___
Significant of study___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ____ ___
CHAPTER TWO
Literature review ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ____ ___
Malaria ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ____ ___
Cause ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ____ ___
Signs and symptoms ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ____ ___
Life cycle ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ____ ___
Diagnosis ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ____ ___
Complications ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ____ ___
Classification ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ____ ___
Prevention ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ____ ___
Malaria parasite ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ____ _
Chloroquine and artesunate combination therapy ___ ___ ___ ___ ___ _
CHAPTER THREE
Materials and methods ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ____ _
Experimental Design ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ____ _
Malaria parasite ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ____ _
Drug administration ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ____ _
Blood collection from animal ___ ___ ___ ___ ___ ___ ___ ___ ___ ___
Laboratory analysis procedure ___ ___ ___ ___ ___ ___ ___ ___ ___ ___
Statistical analysis ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ____ _
CHAPTER FOUR
Result ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ____ __ __ ___ ____
Table 1 ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ____ __ __ ___ ____
Table 2 ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ____ __ __ ___ ____
Table 3 ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ____ __ __ ___ ____
Table 4 ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ____ __ __ ___ ____
CHAPTER FIVE
Discussion ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ____ __ __ ___ _
Conclusion ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ____ __ __ ___ __
Recommendation ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ____ __ ___
Reference ___ ___ ___ ___ ___ ___ ___ ___ ___ ___ ____ __ __ ___ __
Efe, A. (2018). EFFECT OF COMBINED TREATMENT WITH CHLOROQUINE AND ARTESUNATE ON WHITE BLOOD CELL COUNT OF PLASOMDIUM BERGHEI INFECTED ALBINO MICE. Afribary. Retrieved from https://afribary.com/works/effect-of-combined-treatment-with-chloroquine-and-artesunate-on-white-blood-cell-count-of-plasomdium-berghei-infected-albino-mice
Efe, Agbonayinma "EFFECT OF COMBINED TREATMENT WITH CHLOROQUINE AND ARTESUNATE ON WHITE BLOOD CELL COUNT OF PLASOMDIUM BERGHEI INFECTED ALBINO MICE" Afribary. Afribary, 15 Mar. 2018, https://afribary.com/works/effect-of-combined-treatment-with-chloroquine-and-artesunate-on-white-blood-cell-count-of-plasomdium-berghei-infected-albino-mice. Accessed 14 Nov. 2024.
Efe, Agbonayinma . "EFFECT OF COMBINED TREATMENT WITH CHLOROQUINE AND ARTESUNATE ON WHITE BLOOD CELL COUNT OF PLASOMDIUM BERGHEI INFECTED ALBINO MICE". Afribary, Afribary, 15 Mar. 2018. Web. 14 Nov. 2024. < https://afribary.com/works/effect-of-combined-treatment-with-chloroquine-and-artesunate-on-white-blood-cell-count-of-plasomdium-berghei-infected-albino-mice >.
Efe, Agbonayinma . "EFFECT OF COMBINED TREATMENT WITH CHLOROQUINE AND ARTESUNATE ON WHITE BLOOD CELL COUNT OF PLASOMDIUM BERGHEI INFECTED ALBINO MICE" Afribary (2018). Accessed November 14, 2024. https://afribary.com/works/effect-of-combined-treatment-with-chloroquine-and-artesunate-on-white-blood-cell-count-of-plasomdium-berghei-infected-albino-mice