Anti-inflammatory and analgesic activities of Alafia barteri and it’s possible mechanisms of action.

Ogoo Okeke Premium 74 PAGES (1690 WORDS) Project 4 Views

ABSTACT


The pharmacological effects of the metanolic root extract of the plant Alafia barteri Oliv. used commonly in West and Central Africa, from Guinea Bissau east to Cameroon and south to Congo, including Nigeria for the treatment of various diseases (including malaria, arthritis, eye infection an inflammation) was evaluated.

The analgesic effect of the metanolic root extract of Alafia barteri (100, 200 and 400mg/kg) was evaluated in mice using Acetic acid induced writhing, Formalin induced paw licking, Hot plate and Hot water tail flick models. The anti-inflammatory effect of the extract was evaluated in mice using Histamine induced paw oedema and Serotonin induced paw oedema models.

The metanolic root extract (100-400mg/kg) significantly (P<0.05) increased pain threshold in acetic acid writhing test, formalin paw licking and prolonged reaction time in hot plate and tail flick test. The effect produced by the extract was comparable to Diclofenac (10mg/kg) and Morphine (10mg/kg) in acetic acid writhing test and formalin paw leaking test respectively, but morphine proved to be more effective than the extract in hot water tail flick but not in hot plate model.

The metanolic root extract (100-400mg/kg) also produced significant (P<0.05) inhibition of oedema, which was comparable to an anti-histamine, Chloropheniramine(10mg/kg) for Histamine induced oedema and an anti-serototonin, Cyproheptadine(10mg/kg) for Serotonin induced oedema.

The results obtained with the metanolic root of Alafia barteri in the pain and anti-inflammatory models, suggest that the extract possesses analgesic and anti-inflammatory effect

Further experiment was carried out to evaluate the mechanism of action of the metanolic root extract of Alafia barteri using Glibenclamide, a potassium channel blocker, Prazosine, a α1 adrenergic antagonist, Yohimbine, a α2 adrenergic antagonist, L-NNA, a nitric oxide synthase inhibitor, and Cyproheptadine, a 5-HT antagonist. This was carried out to determine the participation of Adrenergic system pathway, potassium ATP channel pathway, nitric oxide pathway and serotonergic pathway in the mechanism of action of the extract.

Phytochemical screening of the extract showed that it contains alkaloids, reducing sugar anthraquinones and saponin. The extract was safe when given up to 400mg/kg orally. However when given intraperitoneally, it produced a dose-dependent lethality with and LD50 of 112.7mg/kg after 24 hours of acute single dose. 


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APA

Ogoo, O (2018). Anti-inflammatory and analgesic activities of Alafia barteri and it’s possible mechanisms of action.. Afribary.com: Retrieved July 18, 2018, from https://afribary.com/works/okeke-ogochukwus-project1

MLA 8th

Okeke, Ogoo. "Anti-inflammatory and analgesic activities of Alafia barteri and it’s possible mechanisms of action." Afribary.com. Afribary.com, 16 Apr. 2018, https://afribary.com/works/okeke-ogochukwus-project1 . Accessed 18 Jul. 2018.

MLA7

Okeke, Ogoo. "Anti-inflammatory and analgesic activities of Alafia barteri and it’s possible mechanisms of action.". Afribary.com, Afribary.com, 16 Apr. 2018. Web. 18 Jul. 2018. < https://afribary.com/works/okeke-ogochukwus-project1 >.

Chicago

Okeke, Ogoo. "Anti-inflammatory and analgesic activities of Alafia barteri and it’s possible mechanisms of action." Afribary.com (2018). Accessed July 18, 2018. https://afribary.com/works/okeke-ogochukwus-project1