EVALUATlON OF THE ANTI-BACTERIAL ACTIVITY OF BIOACTIVE FRACTIONS FROM ETHANOL PEEL EXTRACT OF AnanascomosusAGAINST Escherichia coli

ABSTRACT

Antibacterial resistance is not only increasing the healthcare costs, but also the disease severity

and death rates Crom certain infections that could have been avoided, prevented or treated by

newer and more effective antibacterial agents. However, the antibacterial activity of bioactive

constituents of the crude peel extract obtained from Ananascomosusis yet to be known. This is

the research gap the present research wants to fill.

l he general aim of the study was to isolate and identify the anti-bacterial bioactive constituents

in the crude ethanol peel extract of Ananascomosus.

;\ suitable amount ofAnanascomosuspeels were obtained which were then grinded and

pulverized with the help of ethanol solution to obtain a crude ethanol peel extract of

lmmuscomosus. A solvent extraction process on the crude extract was carried out to obtain Jive

fractions which were then tested for antibacterial activity, phytochemical constituent screening

plus MIC and MBC of the bio-active fractions. Of the five fractions obtained from the

A nwwscomosuspee1 extract, only three fractions showed antibacterial activity against

Fsc·herichia coli. The extraction of the pulverized Ananascomosus peels with 95% v/v ethanol

gave a percentage yield of 8. 9%. The preliminary phytochemieal screening of the 5% wiv extract

revealed the presence of alkaloids, flavonoids, saponins, tannins, and steroids constituents.

Therel

fractions obtained from ethanol peel extract of Ananascomosus to inhibit the growth of

Fscherichia coli indicated the presence of chemical constituents of pharmacological importance.

l n conclusion. Ananascomosuspossesses antibacterial properties against the bacteria Escherichia

rn/i which can be utilized in the cure and prevention of bacterial infectious diseases caused by

J:scherichia coli after standardization.