Microbiology Research Papers/Topics

Composition, structure, and functional shifts of prokaryotic communities in response to co-composting of various nitrogenous green feedstocks

Abstract: Background Thermophilic composting is a promising method of sanitizing pathogens in manure and a source of agriculturally important thermostable enzymes and microorganisms from organic wastes. Despite the extensive studies on compost prokaryotes, shifts in microbial profiles under the influence of various green materials and composting days are still not well understood, considering the complexity of the green material sources. Here, the effect of regimens of green composting mater...

Laboratory and Field Performance of Metarhizium anisopliae Isolate ICIPE 41 for Sustainable Control of the Invasive Fall Armyworm Spodoptera frugiperda (Lepidoptera: Noctuidae)

Abstract: To overcome the negative impacts of invasive fall armyworm (FAW), this study assessed the laboratory and field efficacy of aqueous and oil-based formulations of Metarhizium anisopliae ICIPE 41 as an alternative source for FAW management. Three oil formulations, including canola, corn and olive oils and an aqueous formulation of ICIPE 41, were assessed against the second-instar larvae of FAW in the laboratory. Field experiments were also conducted at Mbita and Migori, Kenya, using t...

Multi-omics analyses reveal rumen microbes and secondary metabolites that are unique to livestock species

Abstract: Ruminant livestock, including cattle, sheep, goats, and camels, possess a distinctive digestive system with complex microbiota communities critical for feed conversion and secondary metabolite production, including greenhouse gases. Yet, there is limited knowledge regarding the diversity of rumen microbes and metabolites benefiting livestock physiology, productivity, climate impact, and defense mechanisms across ruminant species. In this study, we utilized metataxonomics and metabo...

Editorial: Microbiota: A Consequential Third Wheel in the Mosquito-Pathogen Relationship

Abstract: Mosquitoes are by far the most important vectors of human disease. There are hundreds of millions of cases of dengue annually, while Chikungunya and Zika have recently caused major outbreaks. Malaria remains a major driver of poverty in sub-Saharan Africa where it is responsible for about 400,000 deaths each year. In addition, about 50 million cases of lymphatic filariasis still occur annually. The microbial communities harbored by mosquitoes have been the focus of great scientific...

Mathematical modelling of the interactive dynamics of wild and Microsporidia MB-infected mosquitoes

Abstract: A recent discovery highlighted that mosquitoes infected with Microsporidia MB are unable to transmit the Plasmodium to humans. Microsporidia MB is a symbiont transmitted vertically and horizontally in the mosquito population, and these transmission routes are known to favor the persistence of the parasite in the mosquito population. Despite the dual transmission, data from field experiments reveal a low prevalence of MB-infected mosquitoes in nature. This study proposes a compartme...

Complete Reference Genome Assembly for Commensalibacter sp. Strain AMU001, an Acetic Acid Bacterium Isolated from the Gut of Honey Bees

Abstract: We report here the genome sequence of a Commensalibacter sp. strain (AMU001) isolated from honey bees (Apis mellifera) from Seychelles. By combining long- and short-read sequencing technologies, we produced the first complete reference genome assembly for the Commensalibacter genus. We anticipate that this will aid future comparative and functional genomic studies.

Bacterial diversity in the intestinal tract of the funguscultivating termite Macrotermes michaelseni (Sjöstedt)

Abstract: Microorganisms in the intestinal tracts of termites play a crucial role in the nutritional physiology of termites. The bacterial diversity in the fungus-cultivating Macrotermes michaelseni was examined using both molecular and culture dependent methods. Total DNA was extracted from the gut of the termite and 16S rRNA genes were amplified using bacterial specific primers. Representatives from forty-one (41) RFLP patterns from a total of one hundred and two (102) clones were sequence...

Genetic Diversity, Distribution, and Genomic Characterization of Antibiotic Resistance and Virulence of Clinical Pseudomonas aeruginosa Strains in Kenya

Abstract: Pseudomonas aeruginosa is a leading cause of nosocomial infections worldwide. It can produce a range of debilitating infections, have a propensity for developing antimicrobial resistance, and present with a variety of potent virulence factors. This study investigated the sequence types (ST), phenotypic antimicrobial susceptibility profiles, and resistance and virulence genes among clinical isolates from urinary tract and skin and soft tissue infections. Fifty-six P. aeruginosa clin...

Antibiogram and Detection of Metallo-Beta-Lactamase Producing Klebsiella Species Isolated from Poultry Feaces in Owo Metropolis with its Legal Critics.

Beta-lactamase enzymes e.g. metallo β-lactamases (MBLs) that produces Gram-negative bacteria is no doubt one of the widely spread resistance strategies which was geared by these group of bacteria towards β-lactam drugs. MBLs hydrolyzes bacteria and resist carbapenems. This study aimed at determining the antibiogram and the production of MBLs by Klebsiella species from poultry feaces in Owo metropolis. From Poultry feaces aseptically collected Klebsiella species was isolated using MacConkey ...

Prevalence and antibiogram of pseudomonas species isolated from selected African catfish (Clarias Gariepinus) ponds in Owo Metropolis

One of the most common sources of protein globally is catfish which are artificially reared in Nigeria. Pseudomonas species are opportunistic in nature, pathogenic Gram negative bacteria and may build multidrug resistant which can cause a serious threat to public health when present in environmental samples. This research was meant to determine the antibiogram of Pseudomonas species and its prevalence isolated from catfish ponds in Owo metropolis. The pond’s water samples were aseptically c...

Isolation, identification and antibiotic resistance pattern of Pseudomonas species isolated from poultry feaces in owo metropolis

Pseudomonas species are most available pathogenic Gram negative bacteria in environmental samples. They are resistant to several drugs, opportunistic pathogen and their presence in the environment poses threat to the public health. The ability of microorganisms to survive in the presence of antimicrobial agents at a situation that can kill or enhance their growth is known as antimicrobial resistance. This has become a serious threat globally, which makes the treatment of infections in humans ...

Isolation, identification and antimicrobial susceptibility profile of Pseudomonas species isolated from pig (Sus scrofa) feces in owo metropolis

Pseudomonas species are common pathogenic Gram negative bacteria frequently found in environmental samples. Pseudomonas species are responsible for different healthcare-associated infections and are inherently resistant to many commonly used antibiotics. This study was aimed at isolating; identifying and determining the antimicrobial susceptibility pattern of Pseudomonas species isolated from pig feaces in Owo metropolis. Freshly passed feacal samples were aseptically collected from apparentl...

Antibiogram and Detection of Metallo-β-Lactamase Producing Escherichia coli Isolated From Cow Dung in Owo Metropolis

This study was carried out to determine the antibiotic susceptibility profile and the production of metallo-β-lactamase (MBL) by Escherichia coli isolated from cow dung in Owo metropolis. The isolation of Escherichia coli was done using MacConkey agar and Eosin Methylene Blue Agar and was conventionally characterized. Antimicrobial susceptibility test of the isolates were by disc diffusion method against ceftazidime (30 μg), cefuroxime (30 μg), gentamicin (10 μg), cefixime (5 μg), ofloxa...

Microbiology of Polyethylene-Packaged Sliced Watermelon (Citrullus lanatus) Sold by Street Vendors in Nigeria

Abstract Ten packaged, freshly sliced watermelon were collected from different street vendors to determine their microbiological quality. Eight different microbial isolates were obtained from the sliced watermelon samples, namely Escherichia coli, Klebsiella aerogenes, Proteus mirabilis, Staphylococcus aureus, Lactobacillus spp., Saccharomyces cerevisiae, Rhizopus stolonifer and Mucor spp. The effects of high density polyethylene (HDP) and low density polyethylene (LDP) packaging bags on the ...

The Assessment of Floral Abundance and Composition of Neni-Nimo Watershed in Anaocha L.G.A. of Anambra State, Nigeria

Abstract The research was carried out on the floral composition of Neni-Nimo watershed between November 2019 and July 2020. The research work aimed to find out the various plant species that inhabit the watershed, their abundance measures and importance values. In this study, the watershed was divided into three catchment areas, the upper, the middle and the lower catchment. Each of the catchment zones was divided into component parts, with an area of (50 m X 30 m) each. The experiment was la...


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