Research Papers/Topics in Microbiology

Comparative Evaluation of Bacteria and Fungi Diseases Associated with Saccharum officinarum (Sugarcane) in Ethnobotanical Garden of Wesley University, Ondo

ABSTRACT The comparative evaluation of the bacterial and fungal diseases associated with Saccharum officinarum (Sugarcane) in ethnobotanical garden of Wesley University, Ondo was carried out through different microbial processes. The objectives of this work were to comparatively evaluate bacterial and fungal diseases associated with sugarcane in rainy and dry seasons, isolation and identification of bacterial and fungal diseases associated with sugarcane and development of disease inventory a...

Prevalence of Hepatitis B serological markers among HIV infected patients

Abstract Abstract Background: Occult hepatitis B infection (OBI) is defined as serologically undetectable hepatitis B surface antigen (HBsAg-ve), despite the presence of circulating HBV DNA (Allain, 2005). It has been documented that one of the frequent complications of HIV infection is hepatitis B co-infection and due to the common methods of transmission of these two viruses, the incidence rate of co-infection is increasing (Chen et al., 2011) Objective: To determine the prevalence of HBV s...

Investigating The Presence Of Staphylococcus Aureus And Escherichia Coli In Dairy Products

INTRODUCTION Dairy products are various products derived from cow’s milk or that of other female mammals such as goat, sheep, yaks, horses, camel. Dairy products include yoghurt, nono (fermented cow’s milk, madara (unfermented cow’s milk, cheese, whey, condensed and evaporated milk.) (cultureforhealth, 2015). Yoghurt, nono, Madara has become so highly valued in humans diet because of their nutritional values and their culinary possibilities (George and pamplon, 2006) Yoghurt is ma...

ISOLATION OF SERRATIA MARCESCENS FROM THE SOIL AND INVITRO PRODIGIOSIN PRODUCTION AS SOURCE OF ANTIBIOTIC, ACTIVE AGAINST OXACILLIN RESISTANT STAPHYLOCOCCUS AUREUS

  ABSTRACT This research work involve the isolation of two isolates titled RMN1 and RMN2, belonging to the Genus Serratia  from two soil samples collected from the following two strategic locations in university of Abuja, permanent site: Termite hill besides Girls hostel dump ground and from Faculty of Agricultural Science farm land. The isolates were morphologically distinct on the basis of spore colour, aerial and substrate mycelium formation and production of diffusible pigment. Isolat...

ISOLATION OF ANTIBIOTIC PRODUCING STREPTOMYCES FROM THE SOIL AS SOURCE ANTIBIOTICS ACTIVE AGAINST ANTIBIOTICRESISTANT STAPHYLOCOCCUS AUREUS

ABSTRACT The study involved the isolation of (2) isolates titled RMN1, RMN2,  belonging to the Genus Serratia  from ( 2) soil samples collected from the following two strategic locations in university of Abuja, permanent site: Termite hill besides Girls hostel dump ground and from Faculty of Agricultural Science farm land. The isolates were morphologically distinct on the basis of spore colour, aerial and substrate mycelium formation and production of diffusible pigment, Isolates were test...

PREVALENCE OF CHIKUNGUNYA VIRUS IgG AMONG CHILDREN AND ADULTS IN GOMBE

ABSTRACT Infection with Chikungunya virus (CHIKV) is highly infectious disease that causes some dermatological manifestations and clinical symptoms. CHIKV is a member of the family Togaviridae. Structurally, it has an encapsidated genome composed of single-stranded RNA. This study is carried out to determine the seroprevalence and prevalence of CHIKV among the residents of Gombe state. Total number of 90 samples was collected randomly from both sexes between the age group of 2-70 years. The b...

ANTIFUNGAL EFFECT OF GARCINIA KOLA

ABSTRACT The study determines the antifungal effects of aqueous, ethanolic and methanolic extracts of Garcinia kola on some selected fungal isolates and their phytochemical constituents. The antifungal sensitivity and Minimum Inhibitory Concentration (MIC) were determined by agar well diffusion and agar dilution methods, respectively using Sabouraud dextrose agar. The minimum fungicidal concentration (MFC) was determined using samples with no visible growth in the MIC assay and sub-cultur...

A COMPARATIVE STUDY OF THE ANTIBACTERIAL ACTIVITIES OF MEDICATED SOAPS ON SOME SELECTED BACTERIA FROM THE SKIN

Four medicated soaps (Ghana soap, Tetmosol, Beneks’, and Crusader) were investigated for their antibacterial activities against Staphylococcus aureus and Staphylococcus epidermis. A total number of thirty (30) students of Western Delta University, Oghara, Delta state, 15 males and 15 females were skin swabbed with sterile swab sticks. Identification of the bacterial species was by standard microbiological techniques. Crusader soap had the mean highest antibacterial activity (15.3 mm) agains...

CULTIVATION OF PLEUROTUS PULMONARIUS ON SAWDUST

ABSTRACT Pleurotus species are popularly known as oyster mushroom which are regarded as one of the edible mushroom of commercial important throughout the whole world. Utilization of this depends on the ability to secrete cellulolytic and hemilocellulolytic enzymes which can make use of wide spectrum of Agro industrial wastes for the growth and frutification. In this study potato dextrose agar was the only growth medium used to prepared the mycelia of P. pulmonarius. Then the culture was tran...

ISOLATION AND IDENTIFICATION OF MICROORGANISMS RESPONSIBLE FOR SPOILAGE OF DAIRY PRODUCT (MILK, YOGHURT AND LOCALY PREPARED CHEESE)

ABSTRACT Milk, Yoghurt and Cheese are highly valuable food which are readily digested and have high concentration of nutrients which have proved to be a heaven of microbes, studies were conducted on the isolation and identification of microbes [Fungi and Bacteria] on peak canned milk, yoghurt, locally prepared cheese and cheese water. It was revealed that yoghurt was devoid of microbes while milk, locally prepared cheese and cheese water harboured microbes. Locally prepared cheeses, cheese ...

INCIDENCE STUDY OF ROTAVIRUS ANTIGEN IN DIARRHEIC STOOL OF CHILDREN ATTENDING SABANA SPECIALIST HOSPITAL IN GOMBE, NIGERIA

ABSTRACT  Rotavirus is a common cause of diarrhea in children. This study was carried out to determine the prevalence of rotavirus antigen in children below five years presenting with diarrhea in Gombe State. Thirty (30) stool samples were analysed using ELISA KIT. Out of the total number of samples analyzed, a prevalence of 6.67% was recorded all of which are between the age group 7-12 months. A more comprehensive survey is recommended to depict true states of rotavirus as an etiology o...

NEWCASTLE DISEASE VIRUS ANTIBODIES AMONG BIRDS REARED IN ANYIGBA KOGI STATE

TABLE OF CONTENTS CHAPTER ONE 1.0     INTRODUCTION  CHAPTER TWO 2.0     LITERATURE REVIEW CHAPTER THREE 3.0    MATERIALS AND METHODS 3.1    SAMPLE COLLECTION 3.1.1 STERILIZATION OF MATERIALS 3.1.2 PREPARATION OF ERYTHROCYTE FOR HAEMAGGLUTINATION INHIBITION TEST (CHICKEN RED BLOOD CELL) 3.1.3 HAEMAGGLUTINATION ASSAY FOR (STOCK ANTIGEN) 3.1.4 HAEMAGGLUTINATION INHIBITION TEST CHAPTER FOUR 4.1    RESULT CHAPTER FIVE 5.1    DISCUSSION 5.2.1 CONCLUSION / RECOMMENDATION       ...

Extraction Of Alkaloids From Three Nigerian Plants, Kola Accuminata (Oji Igbo), Kola Vera (Oji Hausa), And Gaxcina Kola (Bitter Kola)

ABSTRACT The   extraction of alkaloid from Gaxcina  kola (bitter Kola), Kola acuminated (Oji Igbo) and Kola Vera (Oji Hausa) were carried out using 10% enthanoic acid and 10% ethanol to separate the alkaloid from the residue. The alkaloid was extracted using 5% ammonium hydroxide. From the sample results obtained on alkaloid for the sample 9.16% oji Igbo, 6.20% oji Hausa and 8.20% Bitter kola. It was observed that the percentage of alkaloids was highest in Oji Igbo and the least for Oji Ha...

ISOLATION OF ESCHERICHIA COLI FROM DOMESTIC TANKER WATER SUPPLIES IN ENUGU CITY

ABSTRACT Presence of Escherichia coli from water supplied by water tanker suppliers in Enugu City was determined.  Out of eight different water tanker supplies examined which were designated alphabetically as A, B, C, D, E, F, and H. Escherichia coli was isolated from four tanker suppliers. The membrane filtration technique was used and the presumptive test showed other bacteria such as Klebsiella spp and Bacillus spp  were seen growing in the MacConkery agar. The pink colonies on MacConke...

HEPATOPRODUCTIVE EFFECTS OF SENA TORA LEAVE EXTRACT ON CARBON TETRACHLORIDE INDUCED TOXICITY

TABLE OF CONTENTS CHAPTER ONE 1.0      INTRODUCTION CHAPTER TWO  LITERATURE REVIEW 2.0.       SENA TORA 2.1.1     TAXONOMY OF SENNA TORA [NATURE SERVE, 2007] 2.1.2     GEOGRAPHICAL DISTRIBUTION OF SENNA TORA 2.1.3     GROWTH REQUIREMENTS OF SENNA TORA 2.1.4     PROPAGATION OF SENNA TORA 2.1.5     IMPORTANCE USESOF SENNA TORA 2.2.       LIVER 2.2.1     ANATOMY OF THE LIVER 2.2.2     STRUCTURE OF THE LIVER 2.2.3     FUNCTIONS OF THE LIVER 2.2.4     HEPATIC METABOLI...


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