Microbiology Research Papers/Topics

Microbiological Evaluation of Bacteria Species Isolated from Zobo and Kunnu Sold in Olabisi Onabanjo University

ABSTRACT Zobo and Kunu drinks are non-alcoholic beverage that is widely produced and consumed in Nigeria.The beverage may possess health risk when contaminated due to method of preparation. Zobo and Kunu drinks were purchased from different sellers in Olabisi Onabanjo University, Ago-Iwoye Ogun State. Physical and chemical analysis of the drinks were carried out, involving pour plate were also employed to isolate bacteria from these drinks. The microbial qualities were determined using pour p...

Effects of Palm Oil Processing Waste on Soil

ABSTRACT The microbiological and physicochemical characteristics of soil receiving palm oil processing waste (POPW) in Abraka were investigated. The effect of palm oil processing waste was tested on loamy, sandy and clay soils. The pour plate technique was used for the isolation of the organisms. The total mean bacterial count ranged from 0.91±0.03log10CFU/g to 6.13±0.06log10CFU/g for loamy soil, 0.90±0.02log10CFU/g to 6.09±0.02log10CFU/g for sandy and 0.72±0.04 to 6.0±0.02log10CFU/g fo...

Carriage of β-Lactamase and Antibiotics Resistance Plasmids by Strains of Staphylococcus Aureus Isolated from Hospital Patients in Ekpoma

ABSTRACT A total of 100 subjects were screened for  S. aureus strains, thirty (30) S. aureus isolates were obatained. The carriage of β-lactamase among the 30 isolates was determined by using starch iodine agar plate method. It was found that 19(63.3%) of the S. aureus strains were β-lactamase producing. Plasmid curing of the β-lactamase producing isolates was done using acredine orange supplimented nutrient agar to determine if their β-lactamase producing capability was plasmid mediated...

STUDIES ON THE ANTIBACTERIAL ACTIVITY OF CASTOR OIL SEED ON SOME SELECTED BACTERIA

ABSTRACT  The castor plant is a robust perennial shrub of Euphorbiacaea family.  The seeds are documented to have an antibacterial profile against some bacterial organisms  such as  Escherichia  coli,  Staphylococcus  aureus,  Pseudomonas aeruginosa,    Klebsiella  pneumoniae,  Proteus  vulgaris,  and  Streptococcus pyogenes.  This  study  was  carried  out  to  ascertain  antimicrobial  properties inherent  in  the  extracts  (aqueous,  methanolic  and  n-hexa...

Antimicrobial Effect of Natural Honey and Mango Leave on Pathogenic Bacteria

ABSTRACT  Honey has since been found to possess antibacterial property and is therefore employed for wound and gastro-intestinal disease therapy. This study evaluates the antibacterial spectrum and efficacy of honey and compared with tetracycline.  Different concentrations (50, 75 and 100.0 %) of honey were studied in-vitro using Staphylococcus aureus, Klebsiella spp, Escherichia coli and Citrobacter spp. The data obtained showed the inhibitory action of honey with clear zones of inhibition...

Comparative Evaluation of Bacteria and Fungi Diseases Associated with Saccharum officinarum (Sugarcane) in Ethnobotanical Garden of Wesley University, Ondo

ABSTRACT The comparative evaluation of the bacterial and fungal diseases associated with Saccharum officinarum (Sugarcane) in ethnobotanical garden of Wesley University, Ondo was carried out through different microbial processes. The objectives of this work were to comparatively evaluate bacterial and fungal diseases associated with sugarcane in rainy and dry seasons, isolation and identification of bacterial and fungal diseases associated with sugarcane and development of disease inventory a...

Prevalence of Hepatitis B serological markers among HIV infected patients

Abstract Abstract Background: Occult hepatitis B infection (OBI) is defined as serologically undetectable hepatitis B surface antigen (HBsAg-ve), despite the presence of circulating HBV DNA (Allain, 2005). It has been documented that one of the frequent complications of HIV infection is hepatitis B co-infection and due to the common methods of transmission of these two viruses, the incidence rate of co-infection is increasing (Chen et al., 2011) Objective: To determine the prevalence of HBV s...

Investigating The Presence Of Staphylococcus Aureus And Escherichia Coli In Dairy Products

INTRODUCTION Dairy products are various products derived from cow’s milk or that of other female mammals such as goat, sheep, yaks, horses, camel. Dairy products include yoghurt, nono (fermented cow’s milk, madara (unfermented cow’s milk, cheese, whey, condensed and evaporated milk.) (cultureforhealth, 2015). Yoghurt, nono, Madara has become so highly valued in humans diet because of their nutritional values and their culinary possibilities (George and pamplon, 2006) Yoghurt is ma...

ISOLATION OF SERRATIA MARCESCENS FROM THE SOIL AND INVITRO PRODIGIOSIN PRODUCTION AS SOURCE OF ANTIBIOTIC, ACTIVE AGAINST OXACILLIN RESISTANT STAPHYLOCOCCUS AUREUS

  ABSTRACT This research work involve the isolation of two isolates titled RMN1 and RMN2, belonging to the Genus Serratia  from two soil samples collected from the following two strategic locations in university of Abuja, permanent site: Termite hill besides Girls hostel dump ground and from Faculty of Agricultural Science farm land. The isolates were morphologically distinct on the basis of spore colour, aerial and substrate mycelium formation and production of diffusible pigment. Isolat...

ISOLATION OF ANTIBIOTIC PRODUCING STREPTOMYCES FROM THE SOIL AS SOURCE ANTIBIOTICS ACTIVE AGAINST ANTIBIOTICRESISTANT STAPHYLOCOCCUS AUREUS

ABSTRACT The study involved the isolation of (2) isolates titled RMN1, RMN2,  belonging to the Genus Serratia  from ( 2) soil samples collected from the following two strategic locations in university of Abuja, permanent site: Termite hill besides Girls hostel dump ground and from Faculty of Agricultural Science farm land. The isolates were morphologically distinct on the basis of spore colour, aerial and substrate mycelium formation and production of diffusible pigment, Isolates were test...

PREVALENCE OF CHIKUNGUNYA VIRUS IgG AMONG CHILDREN AND ADULTS IN GOMBE

ABSTRACT Infection with Chikungunya virus (CHIKV) is highly infectious disease that causes some dermatological manifestations and clinical symptoms. CHIKV is a member of the family Togaviridae. Structurally, it has an encapsidated genome composed of single-stranded RNA. This study is carried out to determine the seroprevalence and prevalence of CHIKV among the residents of Gombe state. Total number of 90 samples was collected randomly from both sexes between the age group of 2-70 years. The b...

ANTIFUNGAL EFFECT OF GARCINIA KOLA

ABSTRACT The study determines the antifungal effects of aqueous, ethanolic and methanolic extracts of Garcinia kola on some selected fungal isolates and their phytochemical constituents. The antifungal sensitivity and Minimum Inhibitory Concentration (MIC) were determined by agar well diffusion and agar dilution methods, respectively using Sabouraud dextrose agar. The minimum fungicidal concentration (MFC) was determined using samples with no visible growth in the MIC assay and sub-cultur...

A COMPARATIVE STUDY OF THE ANTIBACTERIAL ACTIVITIES OF MEDICATED SOAPS ON SOME SELECTED BACTERIA FROM THE SKIN

Four medicated soaps (Ghana soap, Tetmosol, Beneks’, and Crusader) were investigated for their antibacterial activities against Staphylococcus aureus and Staphylococcus epidermis. A total number of thirty (30) students of Western Delta University, Oghara, Delta state, 15 males and 15 females were skin swabbed with sterile swab sticks. Identification of the bacterial species was by standard microbiological techniques. Crusader soap had the mean highest antibacterial activity (15.3 mm) agains...

CULTIVATION OF PLEUROTUS PULMONARIUS ON SAWDUST

ABSTRACT Pleurotus species are popularly known as oyster mushroom which are regarded as one of the edible mushroom of commercial important throughout the whole world. Utilization of this depends on the ability to secrete cellulolytic and hemilocellulolytic enzymes which can make use of wide spectrum of Agro industrial wastes for the growth and frutification. In this study potato dextrose agar was the only growth medium used to prepared the mycelia of P. pulmonarius. Then the culture was tran...

ISOLATION AND IDENTIFICATION OF MICROORGANISMS RESPONSIBLE FOR SPOILAGE OF DAIRY PRODUCT (MILK, YOGHURT AND LOCALY PREPARED CHEESE)

ABSTRACT Milk, Yoghurt and Cheese are highly valuable food which are readily digested and have high concentration of nutrients which have proved to be a heaven of microbes, studies were conducted on the isolation and identification of microbes [Fungi and Bacteria] on peak canned milk, yoghurt, locally prepared cheese and cheese water. It was revealed that yoghurt was devoid of microbes while milk, locally prepared cheese and cheese water harboured microbes. Locally prepared cheeses, cheese ...


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