Research Papers/Topics in Molecular Biology And Biotechnology

Diversity Of Extended-Spectrum Beta-Lactamase Genes In Avian Pathogenic Escherichia Coliin Scavenging Local Chicken In Morogoro Municipality, Tanzania

ABSTRACT The poultry industry, especially chicken production has in recent times faced a major setback due to devastating effects of Extended Spectrum Beta-Lactases (ESBL) producing organisms.This research aimed at investigating the diversity of ESBL genes in avian pathogenic Escherichia coli(APEC) among scavenging local chickens. A total of 400 cloacal and oropharyngeal swabs were obtained, out of which 192 Escherichia coli were isolated. By use of virulence factor profiling, these 192 ...

Association Of Betaine Aldehyde Dehydrogenase 2.1 (Badh2.1) Gene Allele With Aroma In Popular Traditional Rice Varieties In Tanzania

ABSTRACT Aromatic rice is highly cherished in many countries of the world and commands premium prices at all levels of the global rice trade. The presence of aroma in aromatic rice is controlled by betaine aldehyde dehydrogenase 2.1 (BADH2.1) gene allele which results from an eight base pair deletion and three single nucleotide polymorphisims (SNPs) in exon seven of betaine aldehyde dehydrogenase 2 (BADH2) gene. This mutation is responsible for the introduction of premature stop codon w...

Molecular Epidemiology Of Leptospira Species Among Agropastoral Communities Living In Katavi-Rukwa Ecosystem, Tanzania

ABSTRACT Leptospirosis is an emerging zoonotic infectious disease that affects humans and animals worldwide as it causes febrile illness in humans. The disease has been reported in a number of human-livestock-wildlife interfaces of Northern and Eastern Tanzania. Very little is known of many zoonotic disease conditions in the research naive areas of Western and Southern Tanzania. This study aimed at detecting the prevalence of Leptospira species among agro-pastoralists at the human-anima...

Genetic diversity of rice (oryza sativa l.) Landraces conserved at the national genebank as revealed by simple sequence repeat (ssr) dna markers

EXTENDED ABSTRACT There is high degree of rice diversity in Tanzania. Increased human population pressure and activities has caused the population of wild rice to disappear at alarming rate. NPGRC collected over 125 accessions of rice landraces and 7 wild relatives for conservation and sustainable utilization in improvement programs, but no morphological or genetic diversity study that have been done to support any future collections. An investigation was conducted to determine the ...

Relationship Between Assembly Behavior And Molecular Responses In Foveal Gland Of Unfed And Fed Adult Rhipicephalus Appendiculatus

ABSTRACTIxodid ticks respond to a limited spectrum of stimuli in their search for hospitable environments, hosts and mates hence change in their behavior. This responsiveness is mediated by pheromones which are signaled by biochemical changes that occur during different stages of feeding. Genes induced during blood feeding result in the expression of new proteins secreted into tick foveal gland. Some of these proteins may be involved in the biosynthesis of these pheromones. Assembly behavior ...

Assessment Of Genetic Diversity Of Maize Landraces In Tanzania Using Random Amplified Polymorhic Dna Markers

ABSTRACT The knowledge and comprehension of the genetic variation of maize (Zea mays L.) landraces is pivotal for the implementation of measures to address conservation and improvement. The purpose of this study was to assess the genetic diversity and relationship among selected maize genotypes in Republic of Tanzania by screening twenty Random Amplified Polymorphic DNA molecular markers.DNA was extracted from 160 maize genotypes and PCR was conducted using twelve informative primer...

Detection Of Arenaviruses In Rodents, Shrews And Elephant Shrews From Selected Wildlife-Human Interfaces In Tanzania

ABSTRACT The present study was conducted to investigate the presence of Arenaviruses from rodents, shrews and elephant shrews captured in selected wildlife-human interfaces in Tanzania. The study involved six sites with high potential for contact between wildlife and humans namely; Ruaha, Kilombero, Mtwara, Mbeya, Mbinga and Mikumi. A total of 121 animals comprising 111 rodents, 3 shrews and 7 elephant shrews were screened for Arenaviruses using conventional Polymerase Chain Reactio...

Application Of Real-Time Rt-Pcr Assay For Detection And Typing Of Foot-And-Mouth Disease Virus In Tanzania

ABSTRACTSpeed is paramount in the diagnosis of highly infectious Foot-and-mouth disease (FMD). Recent developments in molecular biology have enabled specific detection of FMD virus by real-time RT-qPCR and sequencing in endemic settings in Africa. In this study, a laboratory-based experimental design was used to standardize real-time RT-qPCR assay for detection and typing of FMDV in selected regions of Tanzania. The optimized conditions for both pan-serotypic and serotype-specific real-time R...

Adaptation Of Reverse Transcription Loop- Mediated Isothermal Amplification For Field Diagnosis Of Footand- Mouth Disease In Tanzania

ABSTRACT Foot-and-mouth disease (FMD) is a highly contagious viral vesicular disease of cloven hoofed animals and poses major constraints to international trade in livestock production. Methods available for detection of FMD virus (FMDV) require specialized laboratory facilities and equipment. In this study, targeted laboratorybased experiments studies were conducted using reverse transcription loop-mediated isothermal amplification (RT-LAMP) for detection and serotyping of FMDV under f...

The Prevalence Of Methicillin-Resistant Staphylococcus Aureus (Mrsa) Isolated From Raw Bovine Milk In The Morogoro Municipality, Tanzania

ABSTRACT Methicillin-Resistant Staphylococcus aureus (MRSA) have the ability to cause superficial skin infections and occasionally causes invasive and serious diseases. This study was performed in a cross sectional design to determine the prevalence and molecular characterization of MRSA in raw bovine milk in the Morogoro Municipality. Raw milk samples (117) were collected from 18 administrative wards and cultured on Baird-Parker agar to isolate S. aureus. Presumptive colonies were ana...

Detection Of Arenaviruses In Rodents, Shrews And Elephant Shrews From Selected Wildlife-Human Interfaces In Tanzania

ABSTRACTThe present study was conducted to investigate the presence of Arenaviruses from rodents, shrews and elephant shrews captured in selected wildlife-human interfaces in Tanzania. The study involved six sites with high potential for contact between wildlife and humans namely; Ruaha, Kilombero, Mtwara, Mbeya, Mbinga and Mikumi. A total of 121 animals comprising 111 rodents, 3 shrews and 7 elephant shrews were screened for Arenaviruses using conventional Polymerase Chain Reaction (PCR). Th...

Genotypes Of Hepatitis B Virus Among Voluntary Blood Donors In Kinshasa, Democratic Republic Of Congo

ABSTRACTHepatitis B represents a major global health problem. Despite the high endemicity of hepatitis B in Sub-Saharan Africa, little is known about the epidemiology and genotypes of hepatitis B virus (HBV) in Africa. The few published studies that describe the genetic characteristics of HBV in the Democratic Republic of Congo (DRC) involved a very small adult population size. In the present study, epidemiological and molecular characteristics of HBV infection were studied among voluntary bl...

Use Of Molecular Methods To Detect Shedding Of Mycobacterium Bovis In Cattle Faeces And Milk

ABSTRACTBovine tuberculosis (BTB) is a chronic bacterial disease caused by Mycobacterium bovis. It affects cattle and occasionally other animals and human. Several techniques exist for detection of BTB in animals. In Tanzania, Single Intradermal Comparative Tuberculin Test (SCITT) and abattoir meat inspection are the commonly used methods for screening of BTB. This study has used molecular biology method namely; RD4 Real time PCR (RD4 Rt PCR) to detect shedding M. bovis in milk and faecal sam...

Response To Antiretroviral Therapy In Hiv- Infected Patients Attending Care And Treatment Centre In Dar-Es-Salaam, Tanzania

BSTRACTAccess to antiretroviral therapy and Human immunodeficiency virus (HIV) care is increasing in resource-limited settings like Tanzania. This study was conducted to evaluate prevalence and risk factors associated with treatment failure, in HIV patient on Anti Retroviral Therapy (ART) for more than six months in Dar es Salaam. A cross-sectional observational study that involved 70 conveniently sampled and consented HIV-infected patients who were on antiretroviral therapy for more than six...

Seroprevalence And Genetic Characterisationof Peste Des Petitsruminants Virus In Selected Areas Of Tanzania

ABSTRACTPeste des petits ruminants (PPR) is an acute, contagious, fatal disease of domestic and wild small ruminants caused by PPR virus (PPRV). A cross-sectional study was conducted to (i) detect PPRV infection, establish the viral lineage and identify the possible source of its introduction and spread in Morogoro district between November 2014 and January 2015 and (ii) determine presence of PPR in selected villages of Ngorongoro district. Clinical samples of six goats from Morogoro urban an...


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