Frequency of Group B Streptococcus agalactiae ( GBS ) Among Sudanese Pregnant Women with Previous Miscarriage in Khartoum State, 2017

Abstract

Streptococcus agalactiae (S.agalactiae) is one of the most important etiologic agent of several infection,cause abortion, and a present a major public health problem. This study aimed to detect the frequence of S. agalactiae among pregnant women with previous miscarriage by using PCR.Fifty samples of vaginal and high vaginal swabfrom pregnant women , were collected from different hospitals in Khartoum,25 from each vagina and high vagina.

Patients’s data was collected by structured interview questionnaire and analyzed by statistical package for social sciences (SPSS), version 11.5.

DNA was extracted by boiling method and alcohol was used to concentrate the nucleic acid . PCRtechnique was used to amplify the DNA of Group B Streptococci (GBS), the result showed 4 (8%) of each vaginal and high vaginal swabs were positive GBS.

Thirty four (68%) from subjects were normal delivered, 8 (16%) were caesarean section delivery, and 8 (16%) were no previous delivery, Frequency of GBS according to age grouping (19-26), (27-34) and (35-42) were positive ± negative ( 0 ± 11 ; 5 ± 20 ; 3 ± 11) respectively . Mean and standard deviation of agepregnant women, Mean± SD (30.8±5.4) respectively, frequency of Streptococcus agalacticae among pregnant women with previous miscarriage, positive (16%) and (84%) negative, the mean of age according to

freqeunce of Streptococcus agalacticae positive (33%) and (30%) negative. Finally found the frequency of Streptococcus agalacticae in pregnant women with previous miscarriage according to type of delivery 5(10%) normal delivery, 1(2%) caesarean section delivery and 2(4%) no previous delivery.