ABSTRACT
The effectiveness of cotton seed dextrose agar, groundnut seed dextrose agar and kernel dextrose agar in culturing fungi was studied by culturing fungi from yam rot (Dioscorea rotundata). The research demonstrated the choice of the isolated fungi to various media, and revealed that some of the isolated fungi showed the same occurrence in different media. Aspergillus flavus showed preference for kernel dextrose and groundnut seed dextrose agar than Sabouraud dextrose agar and cotton seed dextrose agar. Fusarium oxysporum showed preference to kernel and cotton seed dextrose agar. The occurrence of was only higher in Sabouraud and groundnut seed dextrose agar. In comparison of cultures, the cotton seed dextrose agar culture showed the highest growth of the fungi isolates (66.8±2.35%) while groundnut dextrose agar culture showed the least growth of the fungi isolates (49.8±2.35%). No significant difference in the growth of the fungi isolates was found between cultures (p>0.05). This research demonstrated that groundnut seed and cotton seed dextrose agar can be used to culture specific fungi of interest. Hence, they would provide suitable alternative media for culturing fungi of interest and reduce reliance on potatoes dextrose agar. The use of these media may provide promising interest in research where interest may be to identify, enumerate and characterize fungi.
Keywords: Fungi, Yam rot, Sabouraud, Cotton seed, Groundnut seed, Kernel dextrose agars.
Igwillo, U., VC, N , AE, C & OC, A (2021). Fungal Culture From Yam Rot Using Different Dextrose Agars. Afribary. Retrieved from https://afribary.com/works/fungal-culture-from-yam-rot-using-different-dextrose-agars
Igwillo, Ugochukwu, et. al. "Fungal Culture From Yam Rot Using Different Dextrose Agars" Afribary. Afribary, 05 Nov. 2021, https://afribary.com/works/fungal-culture-from-yam-rot-using-different-dextrose-agars. Accessed 22 Dec. 2024.
Igwillo, Ugochukwu, Nwachukwu VC , Chukwuemeka AE and Ahonsi OC . "Fungal Culture From Yam Rot Using Different Dextrose Agars". Afribary, Afribary, 05 Nov. 2021. Web. 22 Dec. 2024. < https://afribary.com/works/fungal-culture-from-yam-rot-using-different-dextrose-agars >.
Igwillo, Ugochukwu, Nwachukwu VC , Chukwuemeka AE and Ahonsi OC . "Fungal Culture From Yam Rot Using Different Dextrose Agars" Afribary (2021). Accessed December 22, 2024. https://afribary.com/works/fungal-culture-from-yam-rot-using-different-dextrose-agars