Investigation Of Antibacterial Activity, Phytochemical Screening And The Extractive Value Of Different Solvents On Lawson/A Inermij(Henna) Plant Leaf Extract

ABSTRACT.

The purpose of this study was to evaluate the antibacterial activity, to determine the most

efficiency extractive solvents and phytochemical profile of Hot water, Methanol alcohol and

Lemon juice extract of Lawsonia inermis (henna) leaf.

The plant attracted the attention of researchers because of its use as anti-infective agent.

The oily and alcoholic extracts of the plant Lawsonia inermis dried powdered leaves were

separately obtained by maceration, while the aqueous was obtained by Infusion extraction

method.

The bacteria organisms tested were Staphylococcus aureus and Escherichia coli. Extraction,

Phytochemical screening and susceptibility test of the plant leaves were performed using

standard procedures. The Phytochemical screening results revealed the presence of

tannins, steroids and reducing sugars as the major bioactive constituents.

The Lemon juice extractive value was relatively much higher than the alcoholic and

aqueous.

The result of susceptibility test showed that the extract had greater antibacterial effect

ABSTRACT.

The purpose of this study was to evaluate the antibacterial activity, to determine the most

efficiency extractive solvents and phytochemical profile of Hot water, Methanol alcohol and

Lemon juice extract of Lawsonia inermis (henna) leaf.

The plant attracted the attention of researchers because of its use as anti-infective agent.

The oily and alcoholic extracts of the plant Lawsonia inermis dried powdered leaves were

separately obtained by maceration, while the aqueous was obtained by Infusion extraction

method.

The bacteria organisms tested were Staphylococcus aureus and Escherichia coli. Extraction,

Phytochemical screening and susceptibility test of the plant leaves were performed using

standard procedures. The Phytochemical screening results revealed the presence of

tannins, steroids and reducing sugars as the major bioactive constituents.

The Lemon juice extractive value was relatively much higher than the alcoholic and

aqueous.

The result of susceptibility test showed that the extract had greater antibacterial effect

against all the two test organisms with minimum inhibitory concentrations (MICs) used as

compared with standard drugs.

against all the two test organisms with minimum inhibitory concentrations (MICs) used as

compared with standard drugs.