ISOLATION AND CHARACTERIZATION OF Aeromonas spp FROM POND WATER AND FISHES IN ILE-IFE OSUN STATE, NIGERIA.

Ayomide Olayinka 80 PAGES (14917 WORDS) Dissertation
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ABSTRACT

The study was carried out to isolate, characterize and investigate the antibiotic susceptibility pattern of Aeromonas species isolated from pond water and fish samples at Sabo and Iloro pond sites in Ile-Ife, Osun State. 

Physicochemical parameters of the water samples such as pH, temperature, total dissolved solid (TDS), dissolved oxygen (DO) and biochemical oxygen demand (BOD) were determined using standard methods. Aeromonas species was isolated by the spread plate technique for both water and fish intestine samples using Inositol Brilliant Green Bile agar. Antibiotics susceptibility pattern of the isolates was carried out on using the disc diffusion method. 

The physicochemical parameters results showed that the mean TDS values ranging between 238±23.23 and 422±23.23, highest mean pH value of 7.90±0.05 was observed at sabo (pond 2), the result for mean temperature fell within the range of 24˚C and 30˚C, DO values was lowest at iloro with a mean value of 0.24±0.38 and mean BOD values had a range within 0.04±0.42 and 3.01±0.42.  A total of 10 Aeromonas spp were isolated and identified, the antibiotics susceptibility test result revealed that the Aeromonas spp were 100% resistant to augumentin, cefuroxime and cefixime while 80% were susceptible to nitrofurantoin, ciprofloxacin and gentamicin. Also, 90% were susceptible to ofloxacin and only 10% to ceftazidime. 

This research study revealed that water and fish samples could serve as reservoir for Aeromonas spp. Moreso, the decrease in the antibiotics susceptibility to the tested antibiotics indicates the need to take effective measures in regulating activities around the ponds.


TABLE OF CONTENTS

Content                                                                                                                           Page

Title page                  i

Certification                  ii

Dedication                  iii

Acknowledgement                  iv

List of tables                              v

List of figures                  vi

Table of content                  vii

Abstract                  xii

CHAPTER ONE                  1

INTRODUCTION AND LITERATURE REVIEW                              1

Introduction                  1

Aim and objectives                  3

Literature review                  5

      1.3.1 Water                  5

1.3.2 Types of water                  5

1.3.3 Importance of water                  6

1.3.4 Water quality                  7

1.3.5 Aquaculture                              8

1.3.6. Water contamination                  9

1.3.7 Health implications of water contamination                  9

1.3.8 Contamination of water by microorganisms                  10

1.3.9 Genus Aeromonas                  11

1.3.10 Pathogenicity of Aeromonas spp                  12

1.3.11 Epidemiology of Aeromonas spp                  13

1.3.12 Antibiotics susceptibility of Aeromonas spp                  14

CHAPTER TWO                  16

MATERIALS AND METHOD                  16

2.1 Materials                  16

      2.1.1 Reagents                  16

      2.1.2 Glass wares                  16

      2.1.3 Culture media                  16

      2.1.4 Equipment’s                  17

2.2 Method                  17

      2.2.1 Sanitary survey/ study area                  17

      2.2.2 Sterilization and preparation of media and materials                  17

      2.2.3 Sample collection                  17

2.3 Physiochemical analysis                  18

      2.3.1 On site physiochemical analysis                  18

      2.3.1.1 Temperature                  18

      2.3.1.2 pH                  18

      2.3.1.3 Turbidity                  18

      2.3.2 Off site parameters                  18

      2.3.2.1 Dissolved oxygen                              18

      2.3.2.2 Biochemical oxygen demand                  19

2.4 Microbiological analysis                  19

      2.4.1 Enrichment                  19

      2.4.2 Serial dilution                  19

      2.4.3 Characterization and identification of bacterial isolates                  19

      2.4.3.1 Macroscopic morphology                  20

      2.4.3.2 Microscopic morphology                  20

2.5 Physiological and biochemical characterization of bacterial isolates                  20

      2.5.1 Oxidase test                  20

      2.5 2 Catalase test                  21

      2.5.3 Sugar fermentation test                  21

      2.5.3.1 Triple sugar ion test                  21

      2.5.3.2 Mannitol fermentation                  21

      2.5.3.3 Maltose fermentation                  22

      2.5.4 Sodium requirement for growth                  22

      2.5.5 Methyl red test                  22

      2.5.6 Voges proskauer test                  22

      2.5.7 Motility test                  23 

      2.5.8 Indole test                  23

      2.5.9 Decarboxylase test                  23

      2.5.9.1 Lysine decarboxylase test                  23

      2.5.9.2 Arginine decarboxylase test                  24

      2.5.9.3 Ornithine decarboxylase test                  24

2.6 Antibiotics sensitivity testing                  24

2.7 Statistical analysis                  25

CHAPTER THREE                  26

RESULTS                  26

3.1 Description of sampling sites                  26

3.2 Physiochemical analysis for the water samples                  26

      3.2.1 Total dissolved solid                  26

      3.2.2 Temperature                  26

      3.2.3 pH                  27

      3.2.4 Dissolved oxygen (DO)                  27

      3.2.5 Biological oxygen demand                  27

3.3 Microbiological analysis                  31

      3.3.1 Percentage occurrence of isolated Aeromonas spp                  31

      3.3.2 Samples collected from Sabo and Iloro ponds                  31

3.4 Comparing physicochemical quality of water samples collected from                  35

      Sabo and Iloro ponds

3.5 Morphological, cultural and biochemical identification of the                  35

      Aeromonas species

3.6 Antibiotic sensitivity testing of Aeromonas species                  37

      3.6.1 Single resistance pattern in Aeromonas spp                  37

      3.6.2 Multiple resistance pattern in Aeromonas spp                  37

CHAPTER FOUR                  39

4.1 DISCUSSION                  39

4.2 CONCLUSION                  43

4.3 RECOMMENDATION      43

REFRENCES                  45


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APA

Ayomide, O (2019). ISOLATION AND CHARACTERIZATION OF Aeromonas spp FROM POND WATER AND FISHES IN ILE-IFE OSUN STATE, NIGERIA.. Afribary.com: Retrieved September 15, 2019, from https://afribary.com/works/isolation-and-characterization-of-aeromonas-spp

MLA 8th

Olayinka, Ayomide. "ISOLATION AND CHARACTERIZATION OF Aeromonas spp FROM POND WATER AND FISHES IN ILE-IFE OSUN STATE, NIGERIA." Afribary.com. Afribary.com, 10 Jun. 2019, https://afribary.com/works/isolation-and-characterization-of-aeromonas-spp . Accessed 15 Sep. 2019.

MLA7

Olayinka, Ayomide. "ISOLATION AND CHARACTERIZATION OF Aeromonas spp FROM POND WATER AND FISHES IN ILE-IFE OSUN STATE, NIGERIA.". Afribary.com, Afribary.com, 10 Jun. 2019. Web. 15 Sep. 2019. < https://afribary.com/works/isolation-and-characterization-of-aeromonas-spp >.

Chicago

Olayinka, Ayomide. "ISOLATION AND CHARACTERIZATION OF Aeromonas spp FROM POND WATER AND FISHES IN ILE-IFE OSUN STATE, NIGERIA." Afribary.com (2019). Accessed September 15, 2019. https://afribary.com/works/isolation-and-characterization-of-aeromonas-spp