Physicochemical and Phytochemical Analysis of Honey and Shea Butter Samples and Their Antibacterial Effect on Staphylococcus aureus AND Klebsiella pneumoniae
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ABSTRACT
This
research work was carried out to examine the physicochemical and phytochemical
constituents of honey and Shea butter samples respectively and their
antibacterial effect on Staphylococcus aureus and Klebsiella pneumoniae. The physicochemical screening of honey
showed that the honey samples used have low water activity, low moisture
content, low pH value below 4.0 and Ash while the phytochemical analysis of
Shea butter revealed the presence of Saponnins, Tannins, Alkaloids, Steroid and
Phenol. The organisms Staphylococcus aureus and Klebsiella pneumoniae were isolated and identified by standard
microbiological procedures. The antibacterial screening protocol was carried
out by disc diffusion method. The result obtained showed that the honey alone
was able to give an inhibition zone that is up to 29mm and the Shea butter gave
up to 26mm. The synergistic effect of honey and Shea butter showed higher
inhibitory effect of 42mm at 100% concentration. The standard antibiotics;
Ciprofloxacin and Streptomycin had the highest mean of 36mm. The synergism is
hereby recommended for its high antibacterial properties for therapeutic
purposes and can be used as an alternative medicine against Staphylococcus
aureus and Klebsiella pneumoniae infections.
It may be even more conventional to use this natural means as it has very low
antibiotic resistance compared to synthetic antibiotic.
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Anonymous, U (2021). Physicochemical and Phytochemical Analysis of Honey and Shea Butter Samples and Their Antibacterial Effect on Staphylococcus aureus AND Klebsiella pneumoniae. Afribary.com: Retrieved January 24, 2021, from https://afribary.com/works/physicochemical-and-phytochemical-analysis-of-honey-and-shea-butter-samples-and-their-antibacterial-effect-on-staphylococcus-aureus-and-klebsiella-pneumoniae
User, Anonymous. "Physicochemical and Phytochemical Analysis of Honey and Shea Butter Samples and Their Antibacterial Effect on Staphylococcus aureus AND Klebsiella pneumoniae" Afribary.com. Afribary.com, 06 Jan. 2021, https://afribary.com/works/physicochemical-and-phytochemical-analysis-of-honey-and-shea-butter-samples-and-their-antibacterial-effect-on-staphylococcus-aureus-and-klebsiella-pneumoniae . Accessed 24 Jan. 2021.
User, Anonymous. "Physicochemical and Phytochemical Analysis of Honey and Shea Butter Samples and Their Antibacterial Effect on Staphylococcus aureus AND Klebsiella pneumoniae". Afribary.com, Afribary.com, 06 Jan. 2021. Web. 24 Jan. 2021. < https://afribary.com/works/physicochemical-and-phytochemical-analysis-of-honey-and-shea-butter-samples-and-their-antibacterial-effect-on-staphylococcus-aureus-and-klebsiella-pneumoniae >.
User, Anonymous. "Physicochemical and Phytochemical Analysis of Honey and Shea Butter Samples and Their Antibacterial Effect on Staphylococcus aureus AND Klebsiella pneumoniae" Afribary.com (2021). Accessed January 24, 2021. https://afribary.com/works/physicochemical-and-phytochemical-analysis-of-honey-and-shea-butter-samples-and-their-antibacterial-effect-on-staphylococcus-aureus-and-klebsiella-pneumoniae