Prevalence and antibiogram of pseudomonas species isolated from selected African catfish (Clarias Gariepinus) ponds in Owo Metropolis

One of the most common sources of protein globally is catfish which are artificially reared in Nigeria. Pseudomonas species are opportunistic in nature, pathogenic Gram negative bacteria and may build multidrug resistant which can cause a serious threat to public health when present in environmental samples. This research was meant to determine the antibiogram of Pseudomonas species and its prevalence isolated from catfish ponds in Owo metropolis. The pond’s water samples were aseptically collected from three ponds at three different locations in Owo. Centrimide Agar was used for the Isolation of Pseudomonas species, as well as Nutrient Agar, MacConkey Agar, and Eosin Methylene Blue Agar were used for their morphological and cultural characteristics, and the isolates were therefore characterized. Kirby Bauer disc diffusion method was adopted to carry out Antibiogram of the isolates. The isolates indicated different morphological and cultural characteristics. Prevalence of the Pseudomonas species reported were: 43%, 33% and 23% accordingly. The isolates were susceptible to the carbapenem (imipenem, 100%), monobactam (aztreonam, 96%), nitrofuran (nitrofurantoin, 93%), aminoglycosides (gentamicin, 90%), cephems (cefotaxime, 90%, ceftazidime, 93%, cefuroxime, 86%), fluoroquinolones (ciprofloxacin, 70% and ofloxacin, 63%) respectively. meanwhile, the susceptibility of the isolates to beta-lactam combination appeared to be low (augmentin, 30%), while carbapenem (Imipenem) was the most highly active antibiotic, followed by the monobactam (aztreonam). This research revealed that Pseudomonas species were common in catfish ponds and were differently resistant to some of the tested antibiotics, thus poses threat to public health which raises the need for serious monitoring of catfish ponds.