ABSTRACT
Eimeria species are important coccidian parasites worldwide causing significant economic losses
from clinical and subclinical coccidiosis in poultry. Vaccines for its control in Nigeria are
imported without consideration of the local species often resulting in vaccine failure. This study
determined the prevalence of and characterised the Eimeria species present in Oyo and Ogun
States towards an effective control and prevention of coccidiosis.
A semi-structured questionnaire was administered randomly to 166 poultry farmers (82, Oyo; 84,
Ogun) based on the registered members of the Poultry Association of Nigeria, to obtain data on
experience of coccidiosis and the prophylactic measures adopted. Poultry flocks were grouped
into six epizootiological categories comprising: vaccinated and medicated 1-8 week-old (1, 2),
vaccinated and medicated 9-18 week-old (3, 4) and above 19 week-old caged and deep-litter (5,
6). Pooled faecal samples were collected from 9, 9, 9, 20, 54, and 19 chicken flocks in Oyo and
9, 16, 18, 27, 39, and 12 in Ogun belonging to categories 1 to 6 respectively. Oocysts were
isolated by modified flotation method, sporulated in 2.5% potassium dichromate and quantified
by the McMaster method. Eimeria species were identified by morphometry, infection prevalence
and relative abundance was determined. Eimeria DNA was extracted, amplified and quantified
using Real-Time Quantitative Polymerase Chain Reaction (RTQPCR). Data were subjected to
descriptive statistics, Chi-square, Student’s t and Kappa tests. Significance was set at p
OLUFEMI, A (2021). Prevalence And Molecular Characterisation Of Eimeria Species In Commercial Chickens In Oyo And Ogun States, Nigeria. Afribary. Retrieved from https://afribary.com/works/prevalence-and-molecular-characterisation-of-eimeria-species-in-commercial-chickens-in-oyo-and-ogun-states-nigeria
OLUFEMI, ADEYEMI "Prevalence And Molecular Characterisation Of Eimeria Species In Commercial Chickens In Oyo And Ogun States, Nigeria" Afribary. Afribary, 21 Apr. 2021, https://afribary.com/works/prevalence-and-molecular-characterisation-of-eimeria-species-in-commercial-chickens-in-oyo-and-ogun-states-nigeria. Accessed 26 Dec. 2024.
OLUFEMI, ADEYEMI . "Prevalence And Molecular Characterisation Of Eimeria Species In Commercial Chickens In Oyo And Ogun States, Nigeria". Afribary, Afribary, 21 Apr. 2021. Web. 26 Dec. 2024. < https://afribary.com/works/prevalence-and-molecular-characterisation-of-eimeria-species-in-commercial-chickens-in-oyo-and-ogun-states-nigeria >.
OLUFEMI, ADEYEMI . "Prevalence And Molecular Characterisation Of Eimeria Species In Commercial Chickens In Oyo And Ogun States, Nigeria" Afribary (2021). Accessed December 26, 2024. https://afribary.com/works/prevalence-and-molecular-characterisation-of-eimeria-species-in-commercial-chickens-in-oyo-and-ogun-states-nigeria