Isolation, Identification and Antimicrobial Susceptibility test for various bacteria present in blood By Rinku Singh, MSc Microbiology

ABSTRACT 

Bacteremia is one of the most significant public health concerns and is a challenge 

in critical care of a patient. Early diagnosis of bacteremia could be vital step in 

estimating appropriate treatment as well as beginning of it. The current study aims 

to discover the pervasiveness of different bacterial species responsible for causing 

bacteremia around the population of various gender and age group and enabling the 

doctors for choosing the verifiable antimicrobials through antimicrobial 

susceptibility patterns. The diagnosis was achieved by isolation and identification of 

the bacteria, causing infection. 1105 samples of blood were collected from suspected 

patients of different gender as well as age groups. Samples were processed according 

to the standard microbiological protocol during the period from 1 January’21 to 31 

March’21. Out of total screened blood samples, only 110 cultures were found to be 

positive, from which 59 Gram-negative bacterial isolates and 51 Gram-positive 

bacterial isolates were identified, and their antimicrobial susceptibility testing was 

performed through fully automated VITEK2 Compact. Salmonella typhi and 

Salmonella paratyphi A were the most predominant Gram-negative bacteria isolated. 

Staphylococcus epidermis and Staphylococcus hominis were most predominant 

Gram-positive bacteria isolated. Few other Staphylococcus, Enterococcus and E.coli 

were also identified in the remaining isolates. The study reflected that males were 

more suspected to bacteremia as compared to females and age group