Purification and characterization of rhodanese from sprouted maize
Rhodanese, a cyanide detoxifying enzyme, was isolated and partially purified from malted maize (Zea mays) using 85% ammonium sulphate precipitation and ion-exchange chromatography on CM- Sephadex C-25. The specific activity of the enzyme obtained was 5.07RU/mg protein with a yield of 9.6% and purification fold of 2.13. The Michaelis constant (Km) values of the substrates sodium thiolsulphate (Na2S2O3) and potassium cyanide (KCN) were 80 mM and 300mM while their Vmax were 4.86 RU/ml/mi...