Rhodanese, a cyanide detoxifying enzyme, was isolated and partially purified from malted maize (Zea
mays) using 85% ammonium sulphate precipitation and ion-exchange chromatography on CM-
Sephadex C-25. The specific activity of the enzyme obtained was 5.07RU/mg protein with a yield of
9.6% and purification fold of 2.13. The Michaelis constant (Km) values of the substrates sodium
thiolsulphate (Na2S2O3) and potassium cyanide (KCN) were 80 mM and 300mM while their Vmax were
4.86 RU/ml/min and 10.2 RU/ml/min respectively. The enzyme had higher substrate specificity of
100% activity to Sodium thiosulfate than other substrate. The optimum pH and temperature of the
enzyme were 8.0 and 60oC. The enzyme was found to be stable up to 60oC for 40 min. The chloride of
potassium (KCl) at 0.01 and 0.001 M had no effect on the activity of rhodanese from malted maize.
This research showed that rhodanese activity is present in malted maize (Zea mays).
Samuel, I (2019). Purification and characterization of rhodanese from sprouted maize. Afribary.com: Retrieved September 15, 2019, from https://afribary.com/works/purification-and-characterization-of-rhodanese-from-sprouted-maize
Ikuejamoye, Samuel. "Purification and characterization of rhodanese from sprouted maize" Afribary.com. Afribary.com, 10 Jun. 2019, https://afribary.com/works/purification-and-characterization-of-rhodanese-from-sprouted-maize . Accessed 15 Sep. 2019.
Ikuejamoye, Samuel. "Purification and characterization of rhodanese from sprouted maize". Afribary.com, Afribary.com, 10 Jun. 2019. Web. 15 Sep. 2019. < https://afribary.com/works/purification-and-characterization-of-rhodanese-from-sprouted-maize >.
Ikuejamoye, Samuel. "Purification and characterization of rhodanese from sprouted maize" Afribary.com (2019). Accessed September 15, 2019. https://afribary.com/works/purification-and-characterization-of-rhodanese-from-sprouted-maize