Purification and characterization of rhodanese from sprouted maize

Samuel Ikuejamoye 9 PAGES (4438 WORDS) Essay/Paper
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Rhodanese, a cyanide detoxifying enzyme, was isolated and partially purified from malted maize (Zea 

mays) using 85% ammonium sulphate precipitation and ion-exchange chromatography on CM-

Sephadex C-25. The specific activity of the enzyme obtained was 5.07RU/mg protein with a yield of 

9.6% and purification fold of 2.13. The Michaelis constant (Km) values of the substrates sodium 

thiolsulphate (Na2S2O3) and potassium cyanide (KCN) were 80 mM and 300mM while their Vmax were 

4.86 RU/ml/min and 10.2 RU/ml/min respectively. The enzyme had higher substrate specificity of 

100% activity to Sodium thiosulfate than other substrate. The optimum pH and temperature of the 

enzyme were 8.0 and 60oC. The enzyme was found to be stable up to 60oC for 40 min. The chloride of 

potassium (KCl) at 0.01 and 0.001 M had no effect on the activity of rhodanese from malted maize. 

This research showed that rhodanese activity is present in malted maize (Zea mays).

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APA

Samuel, I (2019). Purification and characterization of rhodanese from sprouted maize. Afribary.com: Retrieved June 26, 2019, from https://afribary.com/works/purification-and-characterization-of-rhodanese-from-sprouted-maize

MLA 8th

Ikuejamoye, Samuel. "Purification and characterization of rhodanese from sprouted maize" Afribary.com. Afribary.com, 10 Jun. 2019, https://afribary.com/works/purification-and-characterization-of-rhodanese-from-sprouted-maize . Accessed 26 Jun. 2019.

MLA7

Ikuejamoye, Samuel. "Purification and characterization of rhodanese from sprouted maize". Afribary.com, Afribary.com, 10 Jun. 2019. Web. 26 Jun. 2019. < https://afribary.com/works/purification-and-characterization-of-rhodanese-from-sprouted-maize >.

Chicago

Ikuejamoye, Samuel. "Purification and characterization of rhodanese from sprouted maize" Afribary.com (2019). Accessed June 26, 2019. https://afribary.com/works/purification-and-characterization-of-rhodanese-from-sprouted-maize