Diabetes mellitus, is a metabolic disorder caused by the inability of the beta pancreatic cells to
adequately produce insulin or due to insulin resistance of cells. As a result of the increasingly high
incidence of diabetes globally, the World Health Organization (WHO) has set timelines and
guidelines for the reduction of the risk of mortalities and morbidities associated with noncommunicable diseases including diabetes, by the year 2030. However, this agenda is hinged on
the availability of affordable, safe and effective alternatives for the management and treatment of
these diseases. Hence, there is a need to explore other alternatives to the conventional oral antihyperglycemic agents driven by factors such as patient’s preference, demand among others.
Kombucha is tea fermented by a symbiotic culture of bacteria and yeasts (SCOBY). Consumers
of Kombucha have reported several anecdotal evidences of its medicinal potential. This study
seeks to investigate its anti-diabetic and probiotic effect on alloxan-induced diabetic rats. It was
hypothesized that Kombucha, being a complex matrix of microorganisms and nutraceuticals,
would play an essential role in the management of diabetes.
Molecular characterization of the microbiome of Kombucha using shotgun metagenomics (Oxford
Nanopore MINION sequencing technology) showed Brettanomyces bruxellensis CBS 2499 as the
most abundant species within the microbial community accounting for about 51 % of all reads.
Brettanomyces anomalus, Komagataeibacter xylinus NBRC 15237, Bacillus nealsonii AAU1,
Zygosaccharomyces bailii CLIB 213, Acetobacter, Gluconobacter and over 300 other genera and
species of microorganisms including archaea and viruses were also detected using a combination
of REFSEQ and One Codex data bases (OXCDB). In-vivo experiment was used to evaluate the
anti-diabetic property, safety and gut microbiome changes of Kombucha. Kombucha was found to
perform better than the conventional antidiabetic drugs, metformin and glibenclamide in lowering
the fasting blood glucose (FBG) of the diabetic rats. Daily administration of 25 mg/kg and 100
mg/kg of freeze-dried Kombucha tea demonstrated a 5 fold reduction in FBG (p<0.05) and 40%
and 50% respective increases in body weight of the alloxan-induced diabetic rats compared to the
diabetic control (DC). Histological analysis, shows Kombucha enhances pancreas regeneration
and hence the concomitant increase in insulin secretion as demonstrated in the study. Serum lipid
profiling showed 100mg/kg Kombucha treatment increases the levels of total cholesterol (16%),
high density lipoproteins (HDL) (13%) and low-density lipoproteins (LDL) (10%) but conversely
reduces triglyceride level (17%) compared to the DC (p>0.05).
Further analyses demonstrated that Kombucha decreases the relative organ (liver and kidney) to
body weight ratio in treated animals. In addition, Kombucha was able to reduce significantly the
elevated levels of liver enzymes such as Alkaline phosphatase (ALP), Alanine transaminase (ALT)
and Aspartate Aminotransferase (AST) as well as renal toxicity indices, creatinine and urea in
treated animals. Histology of the kidney and liver also showed that Kombucha has no adverse
effect on the morphology and cellular integrity of these organs suggesting its hepatoprotective and
renal protective potentials. Urinanalysis also showed reduction of glucose in urine for the 100
mg/kg Kombucha-treated animals. Additionally, Kombucha protects the gut microbiome, most
significantly by enhancing the Lactobaccillaceae family of bacteria within the gut and reduces the
possibilities of colonization of the gut by other opportunistic bacterial species.
The study demonstrated that Kombucha is enriched with diverse microbial population with
probiotic value and daily intake of Kombucha may be potentially helpful in the management of
diabetes, protection against renal and liver toxicity and offer gut microbiome protection.
Edu, F (2021). Anti-Diabetic And Probiotic Effect of Kombucha on Alloxan-Induced Diabetic Rats. Afribary.com: Retrieved April 16, 2021, from https://afribary.com/works/anti-diabetic-and-probiotic-effect-of-kombucha-on-alloxan-induced-diabetic-rats
Frontiers, Edu. "Anti-Diabetic And Probiotic Effect of Kombucha on Alloxan-Induced Diabetic Rats" Afribary.com. Afribary.com, 05 Apr. 2021, https://afribary.com/works/anti-diabetic-and-probiotic-effect-of-kombucha-on-alloxan-induced-diabetic-rats . Accessed 16 Apr. 2021.
Frontiers, Edu. "Anti-Diabetic And Probiotic Effect of Kombucha on Alloxan-Induced Diabetic Rats". Afribary.com, Afribary.com, 05 Apr. 2021. Web. 16 Apr. 2021. < https://afribary.com/works/anti-diabetic-and-probiotic-effect-of-kombucha-on-alloxan-induced-diabetic-rats >.
Frontiers, Edu. "Anti-Diabetic And Probiotic Effect of Kombucha on Alloxan-Induced Diabetic Rats" Afribary.com (2021). Accessed April 16, 2021. https://afribary.com/works/anti-diabetic-and-probiotic-effect-of-kombucha-on-alloxan-induced-diabetic-rats