Assessment Of Sensitivity Of P. Falciparrm To Anti Folate Antimalarials In Southern Ghana By Polymerase Chain Reaction (PCR) Assay Systems.

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ABSTRACT

Drug treatment of malaria is being hampered by the emergence of drug resistant strains of malaria parasites. Rapid methods for monitoring patients' response to treatment, applicable to field conditions, would aid management and control of drug resistant P. falciparum infect ions. In this study, mutation specific polymerase chain reaction assays using 3'-mismatched oligonucleotide primers which annealed to the wild or mutated parasites gene encoding dihydrofolate reductase-thymidylate synthase (DHFR-TS) were used to survey P.falciparum strains in two Regions of Southern Ghana (Volta and Greater Accra Regions). Mutations were identified directly from blood samples obtained from patients attending Out Patients Departments in Hospitals. Amplified P.falciparum DHFR-TS nucleotide sequences of 337bps were obtained when reaction proQucts were analyzed by electrophoresis.

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APA

The Public Access, L (2021). Assessment Of Sensitivity Of P. Falciparrm To Anti Folate Antimalarials In Southern Ghana By Polymerase Chain Reaction (PCR) Assay Systems.. Afribary.com: Retrieved May 13, 2021, from https://afribary.com/works/assessment-of-sensitivity-of-p-falciparrm-to-anti-folate-antimalarials-in-southern-ghana-by-polymerase-chain-reaction-pcr-assay-systems

MLA 8th

Library, The Public Access. "Assessment Of Sensitivity Of P. Falciparrm To Anti Folate Antimalarials In Southern Ghana By Polymerase Chain Reaction (PCR) Assay Systems." Afribary.com. Afribary.com, 29 Apr. 2021, https://afribary.com/works/assessment-of-sensitivity-of-p-falciparrm-to-anti-folate-antimalarials-in-southern-ghana-by-polymerase-chain-reaction-pcr-assay-systems . Accessed 13 May. 2021.

MLA7

Library, The Public Access. "Assessment Of Sensitivity Of P. Falciparrm To Anti Folate Antimalarials In Southern Ghana By Polymerase Chain Reaction (PCR) Assay Systems.". Afribary.com, Afribary.com, 29 Apr. 2021. Web. 13 May. 2021. < https://afribary.com/works/assessment-of-sensitivity-of-p-falciparrm-to-anti-folate-antimalarials-in-southern-ghana-by-polymerase-chain-reaction-pcr-assay-systems >.

Chicago

Library, The Public Access. "Assessment Of Sensitivity Of P. Falciparrm To Anti Folate Antimalarials In Southern Ghana By Polymerase Chain Reaction (PCR) Assay Systems." Afribary.com (2021). Accessed May 13, 2021. https://afribary.com/works/assessment-of-sensitivity-of-p-falciparrm-to-anti-folate-antimalarials-in-southern-ghana-by-polymerase-chain-reaction-pcr-assay-systems