ABSTRACT
Drug treatment of malaria is being hampered by the emergence of drug resistant strains of malaria parasites. Rapid methods for monitoring patients' response to treatment, applicable to field conditions, would aid management and control of drug resistant P. falciparum infect ions. In this study, mutation specific polymerase chain reaction assays using 3'-mismatched oligonucleotide primers which annealed to the wild or mutated parasites gene encoding dihydrofolate reductase-thymidylate synthase (DHFR-TS) were used to survey P.falciparum strains in two Regions of Southern Ghana (Volta and Greater Accra Regions). Mutations were identified directly from blood samples obtained from patients attending Out Patients Departments in Hospitals. Amplified P.falciparum DHFR-TS nucleotide sequences of 337bps were obtained when reaction proQucts were analyzed by electrophoresis.
MAK-MENSAH, E (2021). Assessment Of Sensitivity Of P. Falciparrm To Anti Folate Antimalarials In Southern Ghana By Polymerase Chain Reaction (PCR) Assay Systems.. Afribary. Retrieved from https://afribary.com/works/assessment-of-sensitivity-of-p-falciparrm-to-anti-folate-antimalarials-in-southern-ghana-by-polymerase-chain-reaction-pcr-assay-systems
MAK-MENSAH, EPHRAIM "Assessment Of Sensitivity Of P. Falciparrm To Anti Folate Antimalarials In Southern Ghana By Polymerase Chain Reaction (PCR) Assay Systems." Afribary. Afribary, 29 Apr. 2021, https://afribary.com/works/assessment-of-sensitivity-of-p-falciparrm-to-anti-folate-antimalarials-in-southern-ghana-by-polymerase-chain-reaction-pcr-assay-systems. Accessed 19 Apr. 2024.
MAK-MENSAH, EPHRAIM . "Assessment Of Sensitivity Of P. Falciparrm To Anti Folate Antimalarials In Southern Ghana By Polymerase Chain Reaction (PCR) Assay Systems.". Afribary, Afribary, 29 Apr. 2021. Web. 19 Apr. 2024. < https://afribary.com/works/assessment-of-sensitivity-of-p-falciparrm-to-anti-folate-antimalarials-in-southern-ghana-by-polymerase-chain-reaction-pcr-assay-systems >.
MAK-MENSAH, EPHRAIM . "Assessment Of Sensitivity Of P. Falciparrm To Anti Folate Antimalarials In Southern Ghana By Polymerase Chain Reaction (PCR) Assay Systems." Afribary (2021). Accessed April 19, 2024. https://afribary.com/works/assessment-of-sensitivity-of-p-falciparrm-to-anti-folate-antimalarials-in-southern-ghana-by-polymerase-chain-reaction-pcr-assay-systems