Association of some Amino Acids in Serum with Insulin with Secretion among Sudanese patients with type 2 Diabetes Mellitus - study in Khartoum state

A great number of people all over the world suffer from diabetes. Understanding the mechanisms by which amino acids regulate insulin secretion in vivo may reveal novel sites for targeting drugs for the therapy of type 2 diabetes in the future. A descriptive analytical cross-sectional and hospital based study was done. The objectives of this study were to determine in the levels of selected amino acids in patients with diabetes type 2 and to measure the serum levels of insulin in Sudanese patients with diabetes mellitus type 2. Samples were collected from different diabetes centers and hospitals in Khartoum State from October 2012 to January 2014. A total of 167 Sudanese patients with type2 diabetes mellitus were enrolled in this study with age ranged from 20 to 80 years and 47 healthy volunteers (age and sex matched) were involved as control. The study population was divided into males (n =116) and females (n = 98). Venous blood samples were obtained in heparinised tubes after an overnight fast from each participant. Whole blood was put in separate tubes for HBA1C test by ion exchange resin chromatography. Plasma was separated for running the insulin test using ELISA Plasma protein was precipitated by 20% sulfosalicylic acid, centrifuged at 4°C for 15 min at 12000 rpm and the clear supernatant was kept at -80°C until analysis. Plasma glutamate, alanine, luecine, arginine, were determined by automated ion-exchange chromatography with ninhydrin using an amino acid analyzer. Results showed significantly higher levels ofalanine(mean=494.39±242.19)(pvalue=0.000