Characterization of Trypanosomes Isolated from Bovine and Canine Using Polymerase Chain Reaction.

ABSTRACT Trypanosomiasis is a vector-borne parasitic disease, transmitted to animals by tsetse fly (Glossina genus) bites. The human type causes sleeping sickness in man while animal type causes nagana in animals. The aim of this study is to characterize trypanosomes isolated from dogs and cattle in Jos South Abattoir. Isolated trypanosomes were subjected to polymerase chain reaction using kin primers targeting internal transcribed spacer 1 (ITS1) of ribosomal deoxyribonucleic acids. The PCR allowed detection of three trypanosome livestock species namely T. vivax sub-species (150bp, 250bps); T. congolense (750bp) and T. brucei (540bp). The samples from dogs all showed T. vivax sub-species and one sample showed mixed infection of T. vivax sub-species and T. congolense. The samples from cattle showed mostly mixed infections of T. brucei and T. congolense. The most significant finding is the observation that more than one sub-species of T.vivax is circulating in the Jos area.

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APA

OJONE., M (2022). Characterization of Trypanosomes Isolated from Bovine and Canine Using Polymerase Chain Reaction.. Afribary. Retrieved from https://afribary.com/works/characterization-of-trypanosomes-isolated-from-bovine-and-canine-using-polymerase-chain-reaction

MLA 8th

OJONE., MERCY "Characterization of Trypanosomes Isolated from Bovine and Canine Using Polymerase Chain Reaction." Afribary. Afribary, 18 Oct. 2022, https://afribary.com/works/characterization-of-trypanosomes-isolated-from-bovine-and-canine-using-polymerase-chain-reaction. Accessed 27 Apr. 2024.

MLA7

OJONE., MERCY . "Characterization of Trypanosomes Isolated from Bovine and Canine Using Polymerase Chain Reaction.". Afribary, Afribary, 18 Oct. 2022. Web. 27 Apr. 2024. < https://afribary.com/works/characterization-of-trypanosomes-isolated-from-bovine-and-canine-using-polymerase-chain-reaction >.

Chicago

OJONE., MERCY . "Characterization of Trypanosomes Isolated from Bovine and Canine Using Polymerase Chain Reaction." Afribary (2022). Accessed April 27, 2024. https://afribary.com/works/characterization-of-trypanosomes-isolated-from-bovine-and-canine-using-polymerase-chain-reaction

Document Details
MERCY OJONE. Field: Biochemistry Type: Thesis 79 PAGES (16075 WORDS) (pdf)