Effects Of Kenyan Black Tea Water Soluble Components On Theaflavins Interaction With Antibiotics Against Selected Pathogenic Bacteria

Abstract

Water soluble components derived from black tea contain active antibacterial compounds, that can be utilized in combined antibiotic-herb therapy to combat bacterial resistance. This strategy is more advantageuos than using single therapy as it is more effective and has minimal side effects. However, it is not clear if the major bioactive compounds interacts with other water soluble compounds, which inturn affects their interaction with antibiotics. This research therefore aimed at comparing the antibacterial activities of hot water extract of black tea having 18μg/ml of theaflavins and 18μg/ml of isolated theaflavins and their combination with antibiotics such as ampicillin. Their combined effects with antibiotics were determined using disk diffusion and modified Checkerboard method. The chi-square test was used to test the null hypothesis, which stated that water soluble components have no effect on theaflavins interaction with antibiotics. The water soluble components of black tea extract were extracted with hot water and theaflavins in it was measured using Flavognost method. Similar amount of theaflavins were extracted using organic solvents and silica gel column chromatography. The concentrates of hot water extract and isolated theaflavins showed synergistic activity with selected antibiotics. However the level of synergism differed significantly at P

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APA

BOR, A (2021). Effects Of Kenyan Black Tea Water Soluble Components On Theaflavins Interaction With Antibiotics Against Selected Pathogenic Bacteria. Afribary. Retrieved from https://afribary.com/works/effects-of-kenyan-black-tea-water-soluble-components-on-theaflavins-interaction-with-antibiotics-against-selected-pathogenic-bacteria

MLA 8th

BOR, ANDREW "Effects Of Kenyan Black Tea Water Soluble Components On Theaflavins Interaction With Antibiotics Against Selected Pathogenic Bacteria" Afribary. Afribary, 27 May. 2021, https://afribary.com/works/effects-of-kenyan-black-tea-water-soluble-components-on-theaflavins-interaction-with-antibiotics-against-selected-pathogenic-bacteria. Accessed 29 May. 2024.

MLA7

BOR, ANDREW . "Effects Of Kenyan Black Tea Water Soluble Components On Theaflavins Interaction With Antibiotics Against Selected Pathogenic Bacteria". Afribary, Afribary, 27 May. 2021. Web. 29 May. 2024. < https://afribary.com/works/effects-of-kenyan-black-tea-water-soluble-components-on-theaflavins-interaction-with-antibiotics-against-selected-pathogenic-bacteria >.

Chicago

BOR, ANDREW . "Effects Of Kenyan Black Tea Water Soluble Components On Theaflavins Interaction With Antibiotics Against Selected Pathogenic Bacteria" Afribary (2021). Accessed May 29, 2024. https://afribary.com/works/effects-of-kenyan-black-tea-water-soluble-components-on-theaflavins-interaction-with-antibiotics-against-selected-pathogenic-bacteria

Document Details
ANDREW BOR Field: Biochemistry Type: Thesis 107 PAGES (20450 WORDS) (pdf)